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Paenibacillus polymyxa DX32 and application thereof in inhibiting plant Glomerella

A technology of polymyx spore-like and anthracnose bacteria, applied in the field of microorganisms, can solve problems such as loss, loss of early prevention and control opportunities, etc., and achieve a good inhibitory effect.

Inactive Publication Date: 2021-04-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, early prevention and control opportunities are often lost sometimes, resulting in certain losses.

Method used

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  • Paenibacillus polymyxa DX32 and application thereof in inhibiting plant Glomerella
  • Paenibacillus polymyxa DX32 and application thereof in inhibiting plant Glomerella
  • Paenibacillus polymyxa DX32 and application thereof in inhibiting plant Glomerella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This example illustrates the isolation and characterization of Paenibacillus polymyxa DX32.

[0022] In the Fritillaria plantation in Daxi Village, Pan'an County, Zhejiang Province, non-susceptible plants were selected from the high-incidence area of ​​Botrytis cinerea, uprooted and put into sampling paper bags. The paper bags were marked and brought back to the laboratory for isolation of plant endophytic fungi. Pick the fritillaria leaves, rinse them with sterile water, drain them, put them in 2% sodium hypochlorite solution for 3 minutes, take them out, wash them with sterile water for 3 times, and then dry them in a sterile environment. After 5 hours, dry the fritillary leaves Cut the leaves with sterilized scissors, about 2mm×2mm in size, pick them up on NA solid medium plates with tweezers, and cultivate them in the dark at 28°C in an incubator. After the colony grows, use an inoculation loop to dip the bacterial colony onto a new NA solid medium plate for streak ...

Embodiment 2

[0026] This example illustrates the determination of the physiological and biochemical characteristics of Paenibacillus polymyxa DX32.

[0027] Use BIOLOG GENIII (operated according to the kit instructions) to measure the physiological and biochemical characteristics indicators such as the utilization of the only carbon source. strain DX 32 See Table 1 for the sole carbon source utilization and chemical substance tolerance of BIOLOG GENIII plates.

[0028] Table 1 Physiological and biochemical characteristics of the DX32 strain, such as the utilization of the only carbon source, determined by the BIOLOG GENIII plate

[0029]

[0030]

[0031] Note: +, positive; -, negative; w, weakly positive.

[0032] As can be seen from the results in Table 1, the antagonistic strain DX of the present invention 32Dextran, D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-turanose, stachyose, D- raffinose, α-D-lactose, D-melibiose, β-Methyl-D glucoside, D-salicin, N-Acet...

Embodiment 3

[0034] This example illustrates the confrontation growth test between Paenibacillus polymyxa DX32 and anthrax pathogen strains.

[0035] Adopt confrontation growth method to measure the antagonistic strain DX of the present invention 32 Inhibitory effect on different anthrax bacteria. Punch out 5mm cakes of different anthrax pathogenic bacteria with a puncher and place them on 1 / 3 of the diameter of the PDA plate, pick DX 32 A single colony of the bacterial strain reaches the symmetrical 1 / 3 of the PDA plate containing the bacterial cake, and the DX is measured after 5 days of constant temperature cultivation at 28°C 32 The size of the inhibition zone between the anthrax colony and record the data, no DX 32 The PDA plate containing bacteria cake was used as blank control.

[0036] Table 2 The antibacterial zone size (mm) of bacterial strain DX32 of the present invention and different anthracnose bacterial colony confrontation growth

[0037]

[0038] It can be seen from...

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PUM

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Abstract

The invention relates to a paenibacillus polymyxa DX32 strain. The preservation number of the paenibacillus polymyxa DX32 strain is CCTCC No: M 2020639. The invention also discloses an application of the paenibacillus polymyxa DX32 in inhibiting plant Glomerella. The paenibacillus polymyxa DX32 disclosed by the invention can be used for effectively inhibiting plant Glomerella, such as Colletotrichum glycines, Glomerella cingulata, Colletotrichum capsici,Colletotrichum orbiculare and Colletotrichum ampelinum, and has a better inhibiting effect on hypha and spore germination.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Paenibacillus polymyxa DX32 and its application for inhibiting plant anthracnose. Background technique [0002] Paenibacillus polymyxa has a certain control effect on various plant diseases such as plant verticillium wilt, chickpea wilt, rape rot, and black pine root rot. The U.S. Environmental Protection Agency (EPA) has listed it as one of the types of microorganisms that can be used commercially. Whether using HY96-2 (P.polymyxa) fermentation broth or the fine-grained agent (KDLD) made of HY96-2, stable control effects have been achieved in the greenhouse and in the field, and the performance of the KDLD fine-grained agent in the field effect is more prominent. Anthracnose is a large class of diseases that often occur in garden plants, mainly harming the leaves of plants, and sometimes also harming stems and twigs. When the damage is serious, the ornamental value of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/01
Inventor 谢昀烨方丽王汉荣武军王连平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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