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Black fungus melanin and preparation and application thereof

A black fungus and melanin technology, applied to the black fungus melanin and its preparation and application fields, can solve the problems of cumbersome post-processing, low extraction yield, insufficient black fungus melanin dissolution, etc., and achieves strong free radical scavenging ability and thermal stability. Good performance, suitable for large-scale preparation and use

Active Publication Date: 2021-04-02
CHINA JILIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Although there have been research reports on the extraction of black fungus melanin, due to the structural characteristics of the multi-layered cell wall of black fungus, the structure is extremely tough, the conventional alkaline or acid extraction yield is low and the post-treatment is cumbersome; cellulase , ultrasonic or microwave-assisted extraction is helpful to the dissolution of black fungus melanin but the dissolution is not sufficient

Method used

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  • Black fungus melanin and preparation and application thereof
  • Black fungus melanin and preparation and application thereof
  • Black fungus melanin and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058]1 material and reagent

[0059]Bacteria bed fungus (HEIWEI 981) sub-entity, purchased from Yanjian Farm in Xiaoxing'anling, Heilongjiang Province. Before use, it was placed at a 50 ° C oven, pulverized, screening a black fungus powder of 150 to 200 mesh, spare.

[0060]HCl, anhydrous ethanol, ethyl acetate, acetone, NaOH, 95% ethanol, n-hexane, benzene, chloroform, ethylene glycol, choline, 1,1-diphenyl-2-bitrane (DPPH) , Hydrogenated, phystonol, ferrozine, ferrozine, ascorbic acid (Vc), 2,2-联 nitrogen-2 (3-ethyl-benzothiazazolysulfonic acid-6 ) Ammonium salt (ABTS), etc. is analyzed;

[0061]Alkaline protease (Vital 350,000 u / g), purchased from Nanning Donghenghua Road Biotechnology Co., Ltd. Alpha-amylase (vitality>2000U / g) from Bacillus, from Shanghai Macillin Biochemical Technology Co., Ltd.

[0062]N2 Wild Beauty Hidden Caenorhabditis Elegans and Escherichiacoli OP50 by Zhejiang University Institute of Animal Science.

[0063]2 experimental instrument

[0064]JP-250A-2 high-speed multi...

Embodiment 2

[0200]The 2G black fungus (150-200 mesh sieve) was placed in a beaker, and 60 ml of deionized water was added to 1: 30 g / ml of material, and after soaking at room temperature overnight (8h), it was frozen in the ultra-low temperature refrigerator at -75 ° C. After 120 minutes, after taking out in a 20 ° C water bath, it was placed in a microwave oven, and the fire-fired fire (500W) was heated 3.5min, repeated 4 times (ultra-low temperature freezing - thaw - microwave heating), freeze dry (initial temperature - 30 ° C, The vacuum 80Pa) obtains 1.85 g of black fungus powder after pre-treatment.

[0201]After the pre-treated black flour 1.5g, deionized water is added, and the amount of the alkaline protease is added 1.5%, the liquid is 70ml: g, pH value 11.0, placed in ultrasonic cleaning, at ultrasonic frequency 30 kHz, ultrasonic power At 300 W, 10 min was extracted under temperature 45 ° C, then heated to a boiling 5 min rtase, centrifugal (12000 r / min, 30min), obtained 91 ml of th...

Embodiment 3

[0203]Put 2G black fungus (150-200 mesh sieve) in the beaker, add 80 mL of deionized water than 1: 40 g / ml, soaking at room temperature overnight (10h), frozen in the ultra-low temperature refrigerator at -75 ° C After 180 minutes, after taking out in a 20 ° C water bath, it was placed in a microwave oven, and heated in a microwave oven, heated 5.5 min, repeated 2 times (ultra-low temperature freezing - thaw-microwave heating), freeze dry (initial temperature - 30 ° C, The vacuum 80Pa) obtains 1.83 g of black fungus powder after pre-treatment.

[0204]The black fungus powder was 1.5 g of the black fungus powder, and deionized water was added. It was 2.5% in the amount of alkaline protease, and the liquid was 80 ml: g, pH 10.0, placed in an ultrasonic cleaning, and the ultrasonic frequency is 30 kHz, ultrasonic power 240W. 90 min was extracted under temperature 50 ° C, then heated to a boiling 5min ulter enzyme, centrifugal (12000 r / min, 30min), obtained 109 ml of supernatant 109ml ...

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Abstract

The invention discloses black fungus melanin and preparation and application thereof. Deionized water and alkaline protease are added into black fungus powder, ultrasonic extraction and centrifugationare performed, and supernatant and precipitate are obtained; concentrating the supernatant to 1 / 3 of the original volume to obtain a supernatant concentrated solution; adding an extracting agent intothe precipitate, carrying out ultrasonic extraction and centrifugation, mixing the supernatant with the supernatant concentrated solution, and carrying out freeze drying to obtain black fungus melanin; the extraction yield of black fungus melanin can reach 12.09%, the removal rate of ABTS < + > is 91.47%, the removal rate of superoxide anion free radicals is 87.12%, the removal rate of hydrogen peroxide is 91.83%, the half lethal days of nematodes are 11.3 d, and the SOD activity and the CAT activity reach 36.2 U / mg and 45.1 U / mg respectively.

Description

[0001](1) Technical field[0002]The present invention relates to a black wood ear melanin and its preparation and application thereof.[0003](2) Background[0004]Auricularia Auricula is a precious medicinal and edible bacteria in my country, has more than 1,000 years of edible history. The wood ear entity presents dark brown because of its rich melanin, which is extremely high, and has anti-virus, anti-aging, prevention and control of health function such as ultraviolet radiation and enhancing human immunity, especially with strong free radical clearance. Natural functional colorants with great development potential, have a wide range of application prospects in pharmaceutical, cosmetics, food, fine chemicals.[0005]Most of the preparation of black fungus is to extract using it in an alkaline solution, and extracts properties of precipitated in an acidic solution. In order to increase the dissolution effect of melanin, most of the solvent extraction method also assists ultrasonic or mic...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54A61P39/06
CPCC09B61/00C09B67/0096A61P39/06
Inventor 张拥军樊任辉汤雨萱黄凌特陈雅
Owner CHINA JILIANG UNIV
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