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Pelargonium odoratissimum endophytic bacillus velezensis and application thereof

A technology of bacillus and Pangpengxiang, applied in the field of microorganisms, can solve problems such as poor control effect, decline of crop resistance, environmental pollution, etc., and achieve the effects of delaying the occurrence of diseases, promoting growth, and good persistence

Active Publication Date: 2021-03-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, continuous cropping of disease-resistant varieties will cause crop resistance decline (Fang Shumin et al., 2002), and the control effect is poor, while chemical pesticide residues have brought serious environmental pollution
At present, there is no report of endophytic bacillus that has a good effect on promoting tobacco growth and can inhibit tobacco bacterial wilt

Method used

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  • Pelargonium odoratissimum endophytic bacillus velezensis and application thereof
  • Pelargonium odoratissimum endophytic bacillus velezensis and application thereof
  • Pelargonium odoratissimum endophytic bacillus velezensis and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The isolation and identification of embodiment 1 Veles bacillus B1

[0032] (1) Isolation and purification of bacterial strains

[0033] Select healthy and well-growing Pangpengxiang plants, and cut off vigorously growing leaves, stems, and roots for the isolation of endophytes. The specific steps of separation are as follows: wash the separation material with clear water, place it in 70% (v / v) ethanol and soak for 30 sec, then move the separation tissue rapidly to soak in 5% (v / v) sodium hypochlorite for 3 min, carry out Disinfect the surface to kill the bacteria on the surface of the tissue; then transfer it to sterile water and wash it 3 times to remove the residual tissue. Cut the isolated material into small pieces with scissors, move it to a petri dish with NA medium, and place 4 pieces of tissue evenly in each dish. After the separation is completed, affix a label to indicate the separation tissue and separation period, and then culture it at a temperature of 2...

Embodiment 2

[0045] The plate antibacterial effect of embodiment 2 Veles bacillus B1

[0046] 1. Antibacterial effect of strain B1 on R. solanacearum of tobacco

[0047] The activated B1 strain was cultured on the LB medium plate for 48 hours, and the bacteria cake was punched out with a 5mm diameter punch, and then inoculated into the medium added with Ralstonia solanacearum. Place them in a 30°C incubator for culture observation, and record the presence or absence of the inhibition zone. After culturing for 3 days, the size of the inhibition zone was measured by the cross method. Each treatment was repeated 3 times, and the treatment without strain B1 was used as the control.

[0048] The results showed that after culturing for 3 days, strain B1 had a good inhibitory effect on tobacco R. solanacearum ( Figure 7 a, b), the inhibition zone can reach 25.7mm. After 6 days, the size of the inhibition zone of the B1 strain was basically unchanged compared with that of the 3-day culture, w...

Embodiment 3

[0056] Example 3 Pot experiment of controlling tobacco bacterial wilt with Bacillus Velez B1

[0057] Tobacco seedlings of the same size were selected for transplanting, and after transplanting for 15 days (1) the B1 bacterial suspension (OD 600 =1.3-1.4) 20mL, water again after 7 days; (2) water plant immunity inducer (Smart Cong) 20mL, water again after 7 days; (3) water plant immunity inducer (Smart Cong) 20mL, and then water again B1 bacterial suspension poured once (OD 600 =1.3-1.4) 20mL, 24h after the treatment, the R. solanacearum suspension (OD 600 =0.3-0.4) 25mL was poured into the tobacco plant soil. The treatment of watering 15mL tobacco solanacearum suspension after normal watering of nutrient solution was used as positive control; the treatment of watering 15mL of sterilized water after normal watering of nutrient solution was used as negative control, and the treatment of watering 15mL of R. Suspension, watered 15mL streptomycin after 12h as the drug control. ...

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Abstract

The invention discloses pelargonium odoratissimum endophytic bacillus velezensis and application of thereof. The name of the bacterial strain is Bacillus velezensis B1, the preservation number of thebacterial strain is GDMCC No: 61296, and the bacterial strain is preserved in Guangdong province microbial strain preservation center of the 59# building of the 100# courtyard of the Xianlie middle road of Guangzhou city on November 19, 2020. The strain belongs to pelargonium odoratissimum endophyte, and has no pathogenicity; the strain has a good inhibition effect on tobacco ralstonia solanacearum, the resistance of tobacco to ralstonia solanacearum can be obviously enhanced, the occurrence of diseases is delayed, and the persistence is good; an obvious promoting effect is achieved on tobaccogrowth; and the strain has an obvious inhibition effect on phytophthora cucumerina, phytophthora capsici, tomato late blight bacteria, papaya anthracnose bacteria, guava fruit rot bacteria, mangosteen fruit rot bacteria, towel gourd fusarium wilt bacteria, flowering cabbage anthracnose bacteria and banana fusarium wilt bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic Bacillus velesii in Pengpengxiang and its application. Background technique [0002] Tobacco bacterial wilt is one of the main diseases of tobacco in tropical and subtropical regions. It has a wide distribution range and poses a huge threat to the production of high-quality tobacco (He Liyuan et al., 1995). Tobacco R. solanacearum can survive in the soil for a long time, which makes the soil the main source of primary infection. The fungus can directly invade the vascular system from the wound of the rhizome of healthy tobacco plants, or invade from the root cap of the secondary root, When R. solanacearum successfully colonizes the xylem, it forms pus on the surface of the root, returns to the soil and re-infects (Araud et al, 1998; McGarvey et al, 1999; Huang et al, 2000; Pan Zhechao, 2010). Tobacco bacterial wilt often co-occurs with root-knot nematode, to...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22
Inventor 冯淑杰薛梅陈泽容梁嘉莉张荣
Owner SOUTH CHINA AGRI UNIV
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