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A kind of ester compound in purslane and its extraction and separation method and application

The technology of an ester compound and a separation method is applied to an ester compound in purslane and the field of extraction and separation thereof, which can solve the problems of low structural novelty and the like, and achieves environmental protection of the process method, significant anticholinesterase activity, Simple operation and quick results

Active Publication Date: 2022-05-17
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many scholars have focused on the determination of the chemical components of purslane, pharmacodynamics and pharmacokinetics, etc. However, there has never been a separation of a new ester compound in purslane and analysis in vitro and in vivo research report
[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

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  • A kind of ester compound in purslane and its extraction and separation method and application
  • A kind of ester compound in purslane and its extraction and separation method and application
  • A kind of ester compound in purslane and its extraction and separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 extracts and separates the ester compound and its extraction and separation method from purslane.

[0036] A kind of ester compound extracted from purslane, molecular formula is C 20 h 34 o 3 , named (7E, 9E)-6-oxoctadecyl-7,9-dienoic acid ethyl ester, the chemical structural formula is:

[0037] .

[0038] Table 1 is the nuclear magnetic data of this new compound: 1 H-NMR with 13 C-NMR in DMSO.

[0039] Table 1. NMR data of the new compounds of the present invention

[0040] .

[0041] The compound is light yellow oil, easily soluble in methanol, insoluble in water. HRESI(+)TOFMS gives m / z: 323.2584[M+H] + The quasi-molecular ion peak has a molecular weight of 323.2586. combine 1 H-NMR, 13 According to C-NMR and DEPT data, it is speculated that the possible molecular formula of the compound is C 20 h 34 o 3 , with an unsaturation of 4. 13 C-NMR spectrum and DEPT spectrum show 20 carbon signals, respectively 2 CH3 (δ: 13.82; 14.10), 12 C...

Embodiment 2

[0050] Example 2 Anti-tumor effect of the new ester compound of the present invention.

[0051] 1 main material.

[0052] 1.1 Drugs and reagents.

[0053] The new ester compounds used in the experiment were prepared by the above method with a purity of 90% to 99%. They were accurately weighed and diluted with DMSO to the required solutions for the following dosage groups. DMEM high-glucose medium, fetal bovine serum (Hyclone Company of the United States); penicillin and streptomycin (Hangzhou Sijiqing Company).

[0054] 1.2 Cell lines.

[0055] Human colon cancer cell Caco-2, human breast cancer cell MCF-7, human gastric cancer cell BGC-823, human lung adenocarcinoma cell SPC-A1, human liver cancer cell BEL-7402, human cervical cancer cell Hela-229, ovarian cancer cell Ho-8910, human oral epidermoid carcinoma cells KB (Shanghai Cell Bank, Chinese Academy of Sciences).

[0056] 1.3 Grouping.

[0057] Divided into control group, experimental group and zero adjustment group ...

Embodiment 3

[0067] Example 3 The anticholinesterase effect of the new compound of the present invention.

[0068] 1. Main materials.

[0069] 1.1 Drugs and reagents.

[0070] The new compound used in the experiment was prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), phosphorus 5,5'- Dithiobisnitrobenzoic acid (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and acetylthiocholine iodide (ATCI, Dalian Meilun Biotechnology Co., Ltd. Ltd.).

[0071] 1.2 Grouping.

[0072] Divided into negative control group, positive control group and experimental group, each group.

[0073] 2 Experimental methods.

[0074] 2.1 Sample preparation.

[0075] Precisely weigh the sample and 0.11 mg of physostigmine, respectively, and use methanol as the solvent to prepare five gradient concentrations of 2.5, 5.0...

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Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application. The present invention provides (7E,9E)-6-oxoctadecyl-7,9-dienoic acid ethyl ester extracted from purslane and its extraction and separation method and application. The ester compounds are sequentially Using alcohol decoction extraction, silica gel column chromatography, polyamide column chromatography, ODS medium pressure column and Sephadex LH-20 purification, liquid phase separation and preparation, the extraction and separation method is simple, fast, and environmentally friendly, and the obtained by this method separation The compound has high purity, and pharmacological experiments have proved that the obtained compound has anti-tumor and anti-cholinesterase effects, so the described new ester compound extracted from purslane and its salt and derivatives can be developed as natural products New traditional Chinese medicine has broad prospects for medical application.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method, specifically an ester compound in purslane and its extraction and separation method and application . Background technique [0002] Purslane (Portulaca oleracea L.), also known as longevity dish, horse amaranth, is a plant of the family Amaranthaceae. Purslane is drought and waterlogging resistant, light and shade resistant, widely distributed, and rich in resources. It has attracted much attention as a wild plant for both medicine and food. The 2015 edition of "Pharmacopia of the People's Republic of China" recorded the dry aerial parts of purslane as medicine. , has the functions of clearing away heat and detoxification, cooling blood to stop bleeding, and stopping dysentery. [0003] Modern pharmacological stud...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/738C07C67/48C07C67/52C07C67/56A61P35/00A61K31/231A23L33/105A23L33/12B01D15/18
CPCC07C69/738C07C67/48C07C67/52C07C67/56A61P35/00A23L33/105A23L33/12B01D15/1892C07B2200/09A23V2002/00A23V2200/308A23V2250/18A23V2250/21A23V2300/38A23V2300/02A23V2300/14A23V2300/50Y02A50/30
Inventor 成泽东陈以国齐姝博孙瑞英锡相顾莹莹
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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