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A kind of nattokinase sustained-release microspheres and preparation method thereof

A technology of nattokinase and slow-release microspheres, which is applied in the direction of biochemical equipment and methods, enzymes, peptidases, etc., can solve the problems of low effective rate, achieve enhanced stability, enhanced sustained release, and maintain thrombolytic activity Effect

Active Publication Date: 2022-03-25
苏州福赛思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the current nattokinase products are processed into concentrates for oral administration, and the dosage forms are mostly tablets or capsules. Into the capsule, the nattokinase preparation made in this way is quickly inactivated in the gastric environment, even if it is protected by viscous substances such as chitosan, gelatin, sodium alginate, cyclodextrin, etc., nattokinase is absorbed into the blood through the digestive tract The efficiency is still low

Method used

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  • A kind of nattokinase sustained-release microspheres and preparation method thereof
  • A kind of nattokinase sustained-release microspheres and preparation method thereof
  • A kind of nattokinase sustained-release microspheres and preparation method thereof

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Effect test

Embodiment 1

[0075] Embodiment 1: chitosan-nattokinase sustained-release microspheres

[0076] (1) Preparation of chitosan-nattokinase sustained-release microspheres

[0077] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0078] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours to make it fully dissolved, left to stand, and made into an aqueous phase with a mass concentration of chitosan of 0.5%;

[0079] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 10 mg / ml;

[0080] 4) dissolving genipin in 30% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 3 mg / ml;

[0081] 5) dispersing nattokinase in water to prepare an aqueous solu...

Embodiment 2

[0088] Optimization of Preparation Conditions of Chitosan-Nattokinase Sustained Release Microspheres

[0089] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0090] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours to make it fully dissolved, left to stand, and made into an aqueous phase with a chitosan mass concentration of 0.5%;

[0091] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 10 mg / ml;

[0092] 4) dissolving genipin in 30% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 3 mg / ml;

[0093] 5) dispersing nattokinase in water to prepare an aqueous solution with a concentration of 50 mg / ml;

[0094] 6) Acco...

Embodiment 3

[0100] Embodiment 3: the preparation of chitosan-nattokinase sustained-release microspheres

[0101] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0102] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours, fully dissolved, left to stand, and made into an aqueous phase with a chitosan mass concentration of 3%;

[0103] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 30 mg / ml;

[0104] 4) dissolving genipin in 90% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 10 mg / ml;

[0105] 5) dispersing nattokinase in water to prepare an aqueous solution with a concentration of 50 mg / ml;

[0106] 6) According to the volume ...

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Abstract

The invention discloses a nattokinase slow-release microsphere and a preparation method thereof, belonging to the technical field of food or medicine. The sustained-release microsphere of the present invention uses chitosan as the core auxiliary material, and the nattokinase is solidified on the core through a cross-linking agent, so as to achieve the effect of enhancing the stability and slow release of the enzyme. On this basis, using mTG enzyme as a cross-linking agent to cross-link casein on the outer layer of the above-mentioned microspheres, forming sandwich-like nattokinase sustained-release microspheres can further improve the gastric acid resistance and prolong the ability of Nattokinase in the microspheres. Duration of action and antithrombotic effect of soybean kinase. The two nattokinase-chitosan microspheres of the present invention can be used as oral preparations for nattokinase, can be added to food and beverages as ingredients, and can also be used to produce nattokinase slow-release microsphere powder or / concentrate. Compared with pure enzyme protein, the nattokinase sustained-release microspheres of the present invention can improve the shelf life of antithrombotic functional food and the bioavailability of functional factors as functional factor additives.

Description

technical field [0001] The invention relates to a nattokinase slow-release microsphere and a preparation method thereof, belonging to the technical field of food or medicine. Background technique [0002] In modern social life, blood in human blood vessels is often in a state of high viscosity, and thrombosis in blood vessels will lead to serious cardiovascular diseases such as cerebral infarction and myocardial infarction, especially to the health of middle-aged and elderly people. , there are 15 million patients with thrombosis in the world, and the potential thrombolytic agent market is 2 billion US dollars, but the current thrombolytic agents, such as urokinase, streptokinase, recombinant tissue plasminogen activator, etc., have short half-life and side effects However, due to various disadvantages such as large size, high price and inability to be administered orally, it is difficult to become an applicable mass drug. Nattokinase has the advantages of good safety, rapi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K38/48A61K47/61A61K47/62A61P7/02A61P9/00A23L33/17
CPCA61K47/6925A61K47/62A61K47/61A61K38/482C12Y304/21062A61P7/02A61P9/00A23L33/17A23V2002/00A23V2200/326
Inventor 杨瑞金张轩仝艳军吕小妹
Owner 苏州福赛思生物科技有限公司
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