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Nattokinase sustained-release microspheres and preparation method thereof

A technology of nattokinase and slow-release microspheres, applied in biochemical equipment and methods, enzymes, peptidases, etc., can solve the problem of low effective efficiency

Active Publication Date: 2021-02-26
苏州福赛思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the current nattokinase products are processed into concentrates for oral administration, and the dosage forms are mostly tablets or capsules. Into the capsule, the nattokinase preparation made in this way is quickly inactivated in the gastric environment, even if it is protected by viscous substances such as chitosan, gelatin, sodium alginate, cyclodextrin, etc., nattokinase is absorbed into the blood through the digestive tract The efficiency is still low

Method used

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  • Nattokinase sustained-release microspheres and preparation method thereof
  • Nattokinase sustained-release microspheres and preparation method thereof
  • Nattokinase sustained-release microspheres and preparation method thereof

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Effect test

Embodiment 1

[0075] Embodiment 1: chitosan-nattokinase sustained-release microspheres

[0076] (1) Preparation of chitosan-nattokinase sustained-release microspheres

[0077] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0078] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours to make it fully dissolved, left to stand, and made into an aqueous phase with a mass concentration of chitosan of 0.5%;

[0079] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 10 mg / ml;

[0080] 4) dissolving genipin in 30% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 3 mg / ml;

[0081] 5) dispersing nattokinase in water to prepare an aqueous solu...

Embodiment 2

[0088] Optimization of Preparation Conditions of Chitosan-Nattokinase Sustained Release Microspheres

[0089] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0090] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours to make it fully dissolved, left to stand, and made into an aqueous phase with a chitosan mass concentration of 0.5%;

[0091] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 10 mg / ml;

[0092] 4) dissolving genipin in 30% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 3 mg / ml;

[0093] 5) dispersing nattokinase in water to prepare an aqueous solution with a concentration of 50 mg / ml;

[0094] 6) Acco...

Embodiment 3

[0100] Embodiment 3: the preparation of chitosan-nattokinase sustained-release microspheres

[0101] 1) The emulsifier and the organic solvent are mixed according to the volume ratio of emulsifier: organic solvent=1:50 to obtain an oil phase, wherein the emulsifier used is span-80, and the organic solvent used is liquid paraffin;

[0102] 2) Chitosan is added to an acetic acid solution with a volume concentration of 3%, magnetically stirred for 2 hours, fully dissolved, left to stand, and made into an aqueous phase with a chitosan mass concentration of 3%;

[0103] 3) dissolving sodium tripolyphosphate in water to prepare crosslinking agent A with a concentration of sodium tripolyphosphate of 30 mg / ml;

[0104] 4) dissolving genipin in 90% ethanol solution to prepare a cross-linking agent B with a genipin concentration of 10 mg / ml;

[0105] 5) dispersing nattokinase in water to prepare an aqueous solution with a concentration of 50 mg / ml;

[0106] 6) According to the volume ...

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Abstract

The invention discloses nattokinase sustained-release microspheres and a preparation method thereof, and belongs to the technical field of foods or medicines. According to the nattokinase sustained-release microspheres disclosed by the invention, chitosan is used as an inner core auxiliary material, and nattokinase is solidified to an inner core through a cross-linking agent, so that the effects of enhancing the stability and the sustained release of enzymes are achieved. On the basis, mTG enzymes are used as the cross-linking agent, and casein is in cross-linking to the outer layers of the microspheres, so that the sandwich-shaped nattokinase sustained-release microspheres are formed, the gastric acid resistance of the nattokinase in the microspheres can be further improved, the action time of the nattokinase can be prolonged, and the antithrombotic effect of the nattokinase can be further improved. Two nattokinase-chitosan microspheres disclosed by the invention can be used as an oral preparation of the nattokinase, can be used as ingredients to be added to foods and beverages, and can also be used for producing nattokinase sustained-release microsphere powder or / concentrated solutions. Compared with pure zymoprotein, the nattokinase sustained-release microspheres disclosed by the invention as a functional factor additive can prolong the shelf life of foods with antithrombotic functions and improve the bioavailability of functional factors.

Description

technical field [0001] The invention relates to a nattokinase slow-release microsphere and a preparation method thereof, belonging to the technical field of food or medicine. Background technique [0002] In modern social life, blood in human blood vessels is often in a state of high viscosity, and thrombosis in blood vessels will lead to serious cardiovascular diseases such as cerebral infarction and myocardial infarction, especially to the health of middle-aged and elderly people. , there are 15 million patients with thrombosis in the world, and the potential thrombolytic agent market is 2 billion US dollars, but the current thrombolytic agents, such as urokinase, streptokinase, recombinant tissue plasminogen activator, etc., have short half-life and side effects However, due to various disadvantages such as large size, high price and inability to be administered orally, it is difficult to become an applicable mass drug. Nattokinase has the advantages of good safety, rapi...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K38/48A61K47/61A61K47/62A61P7/02A61P9/00A23L33/17
CPCA61K47/6925A61K47/62A61K47/61A61K38/482C12Y304/21062A61P7/02A61P9/00A23L33/17A23V2002/00A23V2200/326
Inventor 杨瑞金张轩仝艳军吕小妹
Owner 苏州福赛思生物科技有限公司
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