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Bacillus subtilis, preharvest preparation of edible fungus and its application

A Bacillus subtilis and preharvest treatment technology is applied to improve the postharvest storage quality of edible fungi, and in the field of preharvest preparations for Bacillus subtilis and edible fungi, and can solve the problem of Pseudomonas or Trichoderma contamination and postharvest quality deterioration. It can achieve the effect of maintaining relative stability, reducing the level of membrane lipid peroxidation, and inhibiting postharvest fungal diseases and bacterial diseases.

Active Publication Date: 2022-02-18
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems that the current edible mushrooms are crisp and tender, the respiration intensity is high, the quality deteriorates quickly after harvesting, and the postharvest is prone to "fake hyphae", Pseudomonas or Trichoderma pollution, while the current preservatives are not effective and single, and the cost is high. , the invention provides a strain of Bacillus subtilis, a preparation for preharvest treatment of edible fungi prepared by using the bacterium, its application and a method for improving the quality of postharvest storage of edible fungi, the preparation for preharvest treatment of edible fungi has good effect and simple formula , can be generally applied to various edible fungi, and significantly improve the postharvest storage quality of edible fungi

Method used

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  • Bacillus subtilis, preharvest preparation of edible fungus and its application
  • Bacillus subtilis, preharvest preparation of edible fungus and its application
  • Bacillus subtilis, preharvest preparation of edible fungus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 strain screening

[0047] 1.1 Screening method:

[0048] (1) Preliminary screening: the above-mentioned soil samples or mushroom samples were gradiently diluted with sterile physiological saline to the soil bacterial suspension, and 10 -2 、10 -3 Dilution The dilution is spread on the beef extract peptone medium plate, cultured upside down at 37°C for 1-2 days, and the growth of the bacteria is observed every 12 hours. After the colony on the beef extract peptone plate grows, select the appropriate colony, use a sterilized toothpick to spot the selected colony on the primary screening medium plate, mark it, and place it at 37 ° C for 1-2 days.

[0049] (2) Re-screening: Use the plate confrontation method to judge whether the screened bacterial strains have inhibitory effect on the pathogenic fungus Trichoderma harzianum and white jade mushroom hyphae: pick the bacteria that are screened and place them in a dotted manner with the pathogenic fungus or white jad...

Embodiment 2

[0052] Identification of Example 2 bacterial strain B.subtilis NYB169

[0053] 2.1 Morphological identification results

[0054] Such as figure 1 As shown, the colonies grown by the strain B.subtilis NYB169 on the primary screening plate are about 3mm in diameter, roughly round, off-white, opaque, with a little wrinkle and rough edges. The strain showed blue-purple after staining, so it can be determined that the strain belongs to Gram-positive bacteria.

[0055] 2.2 Physiological and biochemical identification results

[0056] Physiological and biochemical experiments were carried out on B. subtilis NYB169, and the results are shown in Table 1. The strain can hydrolyze tyrosine and starch, and tyrosine crystals can be completely hydrolyzed to become transparent; methyl red (MR) test, peroxidation Hydrogenase test and indole test were both positive.

[0057] Table 1 Physiological and biochemical experiment results

[0058]

[0059] Note: "+" means positive, "-" means n...

Embodiment 3

[0067] The preparation of embodiment 3 edible fungi preharvest treatment preparation

[0068] 1. Cultivation and fermentation of strains

[0069] Fermentation medium: 0.06% potassium dihydrogen phosphate (KH 2 PO 4 ), 0.14% dipotassium hydrogen phosphate (K 2 HPO 4 ), 0.1% magnesium sulfate (MgSO 4 ), 0.06% yeast powder, 0.06% peptone, pH 7.0. Use water as solvent. The above-mentioned percentage content is a mass percentage.

[0070] Insert the strain B. subtilis NYB169 into the seed culture solution, cultivate overnight at 37°C and 150 r / min, and then inoculate the seed solution with the above-mentioned fermentation medium at an inoculation amount of 1% (v / v). In the Erlenmeyer flask, cultivate under the conditions of 37° C. and 150 r / min for 12 to 30 hours to obtain a fermentation broth. Separation of fermentation broth and bacteria by centrifugation or filtration.

[0071] 2. Preparation of preharvest preparations for edible fungi

[0072] After dissolving methyl ...

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Abstract

The invention belongs to the technical field of bioengineering. The invention discloses a strain of Bacillus subtilis, a preharvest preparation for edible fungi and an application thereof. The strain is named Bacillus subtilis NYB169, and was preserved in China Microbial Bacteria on July 06, 2020 Species Deposit Management Committee General Microbiology Center, its deposit number is CGMCC 20186. The Bacillus subtilis can be used to prepare a pre-harvest preparation for edible fungi, and the pre-harvest preparation for edible fungi includes the culture of the above-mentioned Bacillus subtilis, L-arginine, L-cysteine, methyl jasmonate, water Methyl cylate, thyme essential oil, tea polyphenols and CaCl 2 . The edible fungus preharvest treatment preparation can be well combined with postharvest storage means, which not only effectively inhibits the generation of postharvest aerial mycelium of the edible fungus, but also prevents and controls the pollution of Pseudomonas and Trichoderma, and reduces the browning and softening of the mushroom body. Autolysis and other problems, to achieve the effect of improving the postharvest storage quality of edible fungi.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a strain of Bacillus subtilis, a preparation for preharvest treatment of edible fungi using the fungus, its application and a method for improving the postharvest storage quality of edible fungi. Background technique [0002] Edible fungi refer to edible mushrooms (large fungi) with large fruiting bodies, commonly known as mushrooms. Edible mushrooms are not only delicious, but also have high nutritional value. They are important ingredients that are popular among consumers. There are more than 350 kinds of edible fungi known in China, most of which belong to Basidiomycotina. Common edible fungi are: shiitake mushrooms, straw mushrooms, mushrooms, fungus, tremella, Hericium erinaceus, Tricholoma, Ganoderma lucidum, Cordyceps, Bailing mushrooms and boletus. [0003] White jade mushroom, the systematic classification in nature is located in the genus Yuma in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G18/00A01G7/06A23B7/154A23B7/155A23B7/157C12R1/125
CPCA01G18/00A01G7/06A23B7/154A23B7/155A23B7/157C12R2001/125C12N1/205
Inventor 程凡升李文香
Owner QINGDAO AGRI UNIV
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