Kumquat essential oil and preparation method and application thereof
A technology of kumquat essential oil and kumquat essence, which is applied in the field of traditional Chinese medicine, can solve problems such as non-negligible, target organ damage and side effects, and achieve the effects of reducing target organ damage, reducing vasoconstrictive pressure, and easy availability of resources.
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Embodiment 1
[0028] Preparation of kumquat essential oil
[0029] 1. Kumquat raw material: purchased from the Suichuan planting base in Jiangxi Province, identified and reported by Mr. Liu Yong, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, as the fruit of Fortunella Crassifolia Swingle, a plant of the genus Kumquat in the family Rutaceae.
[0030] 2. Preparation method 1) Pre-treatment: After cleaning the fresh kumquat, crush it;
[0031] 2) Dry steam extraction: Weigh 12 kg of kumquat crushed in step 1 and carry out dry steam extraction in the essential oil extraction system device. The extraction time is 2 hours, stop the instrument, and after the steam condenses, use a 10ml syringe to extract the layered liquid. Collect the essential oil layer to obtain crude kumquat essential oil;
[0032] 3) Refining: put the crude kumquat essential oil prepared in step 2 into a brown bottle, seal the bottle cap with a parafilm, refrigerate and let stand overnight at 4°C in...
Embodiment 2
[0034] Determination of Active Components in Kumquat Essential Oil
[0035] 1. Experimental process
[0036] Gas chromatography conditions: Agilent 19091s-433:93.92873 column, 30m×0.25mm×0.25μm. The inlet temperature was 250 °C, the total flow rate was 19.6 mL / min, the split ratio was 20:1, and the column flow rate was 1 mL / min. The interface temperature is 280°C. The heating program is 40°C for 2 minutes, rising to 250°C at 10°C / min, holding for 2 minutes, then rising to 280°C at 20°C / min, and holding for 1 minute. The carrier gas is high purity helium. The injection volume was 1 μL.
[0037] Mass spectrometry conditions: EI ionization source, electron energy at 70eV, ion source temperature at 230°C, quadrupole temperature at 150°C. The measurement method is full scan, the mass range is 35-550u, the multiplier voltage is 1482V, and the filament emission current is 34.6μA. The solvent delay time was 2.0 min.
[0038] Qualitative and quantitative: Use the NIST11.L spectr...
Embodiment 3
[0042] Animal Experiments on Prevention and Treatment of Hypotension
[0043] A single dose of kumquat essential oil 0.5ml / kg was administered, and the systolic blood pressure, serum angiotensin Ⅱ, aldosterone, and vascular endothelial relaxation factor were investigated in rats.
[0044] 1. Experimental protocol
[0045] 1.1 Drug preparation
[0046] Get the kumquat essential oil prepared in Example 1 and dilute it with 2% Tween 80 aqueous solution.
[0047] 1.2 Animals
[0048] SPF grade SD rats, male, weighing (180±10) g, 30 were purchased from the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine [SCXK (Gan) 2018-0003].
[0049] Raised in the experimental animal barrier system [SYXK (Gan) 2017-0004], the laboratory temperature is 23±2°C, the relative humidity is 53±3%, the noise is below 60 decibels, the sunshine is 12h, the animals are free to drink water and eat, and the bedding is changed every day , when the body weight of the animals...
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