Bovine serum albumin modified protoporphyrin/trimanganese tetroxide /carbon oxynitride nano composite material as well as preparation and application thereof
A technology of bovine serum albumin and nanocomposite materials, which is applied in the direction of nanotechnology, nanotechnology, nano-optics, etc. for materials and surface science, can solve the limitation of penetration depth, the influence of MR imaging artifacts on accuracy, and the difficulty Treatment and other problems, to achieve the effect of simple preparation method, integration of diagnosis and treatment, and low price
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Embodiment 1
[0054] A preparation method of bovine serum albumin-modified protoporphyrin / manganese tetroxide / carbon nitride oxide nanocomposite material, comprising the following steps:
[0055] (1) Prepare a uniformly dispersed carbon oxide nitride nanosheet aqueous solution, and the specific steps are as follows:
[0056] (11) 10g of melamine was heated to 550°C at 3°C / min in a muffle furnace, kept for 3h, and cooled to room temperature naturally to obtain graphite phase carbon nitride, which was ground into powder for subsequent use;
[0057] (12) dissolve 1 g of the graphitic carbon nitride obtained in step (11) into 50 mL of concentrated sulfuric acid, slowly add 3.5 g of potassium permanganate under ice bath, and keep stirring; after the potassium permanganate is completely dissolved, transfer In a water bath at 37°C, continue to stir and react for 4 hours; slowly add 200 mL of deionized water to dilute in an ice bath, and finally add 30% hydrogen peroxide solution dropwise until the...
Embodiment 2
[0079] A preparation method of bovine serum albumin-modified protoporphyrin / manganese tetroxide / carbon nitride oxide nanocomposite material, comprising the following steps:
[0080](1) Prepare a uniformly dispersed carbon oxide nitride nanosheet aqueous solution, and the specific steps are as follows:
[0081] (11) heating the melamine to 500°C at 3°C / min in a muffle furnace, maintaining for 4h, and naturally cooling to room temperature to obtain graphitic carbon nitride, which is ground into powder for subsequent use;
[0082] (12) the graphitic carbon nitride obtained in step (11) is dissolved in the concentrated sulfuric acid, and potassium permanganate is slowly added under an ice bath, wherein the moles of graphitic carbon nitride, concentrated sulfuric acid and potassium permanganate are The ratio is 1:80:1.8, stirring constantly; after the potassium permanganate is completely dissolved, transfer to a water bath at 36°C, continue stirring, and react for 4 hours; slowly a...
Embodiment 3
[0094] A preparation method of bovine serum albumin-modified protoporphyrin / manganese tetroxide / carbon nitride oxide nanocomposite material, comprising the following steps:
[0095] (1) Prepare a uniformly dispersed carbon oxide nitride nanosheet aqueous solution, and the specific steps are as follows:
[0096] (11) heating the melamine to 525°C at 3°C / min in a muffle furnace, maintaining for 3.5h, and naturally cooling to room temperature to obtain graphitic carbon nitride, which is ground into powder for subsequent use;
[0097] (12) the graphitic carbon nitride obtained in step (11) is dissolved in the vitriol oil, slowly adding potassium permanganate under ice bath, the molar ratio of graphitic carbon nitride, vitriol oil and potassium permanganate is 1:100:2.2, stirring continuously; after the potassium permanganate is completely dissolved, transfer to a water bath at 39 °C, continue stirring, and react for 4 hours; slowly add 200 mL of deionized water to dilute in an ice...
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