A Strain of Trametes pilosae with Efficient Decolorization of Lignin
A technology of Trametes and lignin, applied in the field of microbial engineering, can solve the problems of low lignin enzyme activity, high enzyme load, and low decolorization efficiency, and achieve the effect of short decolorization time and simple operation
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Embodiment 1
[0052] Embodiment 1: Isolation, screening and species identification of Trametes hirsuta X-13 strain
[0053] 1. Separation and screening
[0054] The fruiting bodies of fungi growing well on dead trees or fallen trees in Yuelu Mountain, Hunan Province were washed with sterile water and wiped with 75% alcohol, and then cut into pieces with a sterilized scalpel in an ultra-clean bench. 0.5cm×0.5cm bacterial block, the bacterial block was inoculated on the PDA agar medium, and placed in a constant temperature incubator at 28°C for cultivation.
[0055] After the fungal mycelium grows on the PDA agar medium, cut off the 0.5cm×0.5cm mycelium with a thin layer of medium with an inoculation spatula, and inoculate it in a new PDA agar medium.
[0056] Obtain pure colonies of microorganisms after repeated inoculation and culture for 2 to 3 generations, inoculate the isolated pure colonies on PDA-aniline blue agar medium and PDA-guaiacol agar medium respectively, and place them in a c...
Embodiment 2
[0062] Example 2: Detection of lignin-degrading enzyme produced by Trametes hirsuta X-13 strain
[0063] The Trametes hirsuta X-13 mycelium obtained in Example 1 was inoculated on the PDA-guaiacol agar medium, and then the PDA-guaiacol agar medium was inverted at 28°C for constant temperature cultivation In the box, cultivate for 3-5 days, observe and record the discoloration of the culture medium around the colony.
[0064] The result is as Figure 5-6 As shown, compared with the control that was not inoculated with Trametes hirsuta X-13, the colonies on the PDA-guaiacol agar medium inoculated with this bacterium had larger and clear reddish-brown around and at the bottom circle, the diameter of the reddish-brown circle is 31 mm, which indicates that the strain has a strong lignin-degrading enzyme ability.
Embodiment 3
[0065] Embodiment 3: the detection of peroxidase produced by Trametes hirsuta (Trametes hirsuta) X-13 strain
[0066] The Trametes hirsuta X-13 mycelium obtained in Example 1 was inoculated on the PDA-aniline blue agar medium, and then the PDA-aniline blue agar medium was placed upside down in a 28°C constant temperature incubator, and cultivated From 3 to 5 days, observe and record the decolorization of the culture medium around the colony.
[0067] The result is as Figure 7-8 As shown, compared with the control, the blue color of the PDA-aniline blue agar medium inoculated with the bacterium almost disappeared, the diameter of the faded circle was 55mm, and the hyphae still showed natural color, which indicated that the bacterium could degrade and utilize Aniline blue, instead of adsorbing aniline blue, which indicates that the strain has a strong ability to produce lignin peroxidase or manganese peroxidase.
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