Immobilized metal chelate chromatography medium, and preparation method and application thereof
An immobilized metal, chelation chromatography technology, applied in chemical instruments and methods, separation methods, other chemical processes, etc., can solve the problems of increasing the preparation cost, cumbersome operation process, difficult quantitative control, etc. Good application prospects and the effect of increasing the quantity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0030] A preparation method for an immobilized metal chelate chromatography medium may comprise the following steps:
[0031] Step 1. Hydrolyzing the macroporous polymer microspheres under acidic conditions.
[0032] Step 2. Swelling the macroporous polymer microspheres treated in step 1 in the functional monomer solution.
[0033] Step 3. Stir and mix the initiator and the material treated in step 2 in a solvent, carry out graft polymerization reaction at a certain pH value and a certain temperature, and then wash away soluble substances with deionized water.
[0034] Step 4, putting the material treated in step 3 into a metal ion solution of a certain concentration and soaking for a certain period of time, thereby obtaining an immobilized metal chelation chromatography medium.
Embodiment approach
[0036] (1) In step 1, the macroporous polymer microspheres can be polyacrylate microspheres or polystyrene microspheres; the polyacrylate microspheres can be polyacrylate modified products, Derivative products or graft copolymers; the polystyrene microspheres can be polystyrene modified products, derivative products or graft copolymers; for example: the polyacrylate microspheres preferably adopt methacrylic acid shrinkage Copolymerized microspheres of glyceride and ethylene glycol dimethacrylate or self-polymerized microspheres of ethylene glycol dimethacrylate. This type of macroporous polymer microsphere can be prepared by atom transfer radical polymerization; this type of macroporous polymer microsphere is porous and interconnected, with a pore size range of 100-3000 nm and a particle size of 20-200 μm. The strength is above 1.0MPa, and the specific surface area is 5-200m 2 / g, using this kind of macroporous polymer microspheres with high mechanical strength, large pore si...
Embodiment 1
[0055] A kind of immobilized metal chelation chromatography medium, its preparation method can comprise the following steps:
[0056] Step A1, macroporous PGMA-EDMA microspheres are hydrolyzed under acidic conditions:
[0057] Accurately weigh 2.0 g of macroporous PGMA-EDMA microspheres into a 50 mL Erlenmeyer flask, then add 10 mL of dilute sulfuric acid solution with a concentration of 0.5 mol / L, and seal and vibrate for 3 h at room temperature in a vibrating shaker at 120 rpm. Suction filtration and washing with the same volume of deionized water, after washing, the solvent is suction filtered until the surface of the microspheres is neutral.
[0058] Step A2, preparation of composite monomer solution:
[0059] Accurately weigh sodium hydroxide and iminodiacetic acid (IDA), a tridentate chelating ligand, with a molar ratio of 2:1, and add an appropriate amount of water to completely dissolve it to obtain iminodiacetic acid sodium salt solution; slowly pour the iminodiacet...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
mechanical strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com