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Rooting-promoting Bacillus sporothermodurans and application of rooting-promoting Bacillus sporothermodurans

A technology of heat-resistant bacillus and bacillus, applied in the field of microorganisms, can solve the problem that elements such as phosphorus and potassium cannot be absorbed and utilized by plants, and achieve the effects of shortening the plant growth cycle, facilitating compost fermentation, and enhancing stress resistance

Inactive Publication Date: 2020-08-21
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, it is necessary to provide a bacterial strain that promotes growth, decomposes phosphorus and nitrogen, and solves the problem that elements such as phosphorus and potassium in the soil cannot be absorbed and utilized by plants.

Method used

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  • Rooting-promoting Bacillus sporothermodurans and application of rooting-promoting Bacillus sporothermodurans
  • Rooting-promoting Bacillus sporothermodurans and application of rooting-promoting Bacillus sporothermodurans
  • Rooting-promoting Bacillus sporothermodurans and application of rooting-promoting Bacillus sporothermodurans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Phosphate solubilization of Bacillus

[0025] 1. Test materials

[0026] 1.1 Test strain

[0027] Test strain: Bacillus FJAT-49377, isolated from pig farm litter of Nanyang Industrial Company, Qidu Town, Ningde City, Fujian Province, cryopreserved in glycerol at -80℃, and deposited in the General Microbiology Center of China Microbial Culture Collection Management Committee, deposit number CGMCC NO.16330.

[0028] 1.2 Medium

[0029] Activation medium: (1) LB solid medium (purchased from Shenggong) formula: tryptone 10g, sodium chloride 10g, yeast powder 5g, agar 15g, water 1000mL, pH 7.0. (2) Formula of LB liquid medium (purchased from Shenggong): tryptone 10g, sodium chloride 10g, yeast powder 5g, water 1000mL, pH 7.0.

[0030] Inorganic phosphorus medium (NBRIP medium): glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 ·6H 2 O 5g, KCl0.2g, MgSO 4 ·7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, 1000mL distilled water, natural pH.

[0031] Organophosphorus growth medium: glucose 10g, ammoniu...

Embodiment 2

[0078] Example 2 Growth-promoting effect of Bacillus

[0079] 1. Tomato growth promotion test method

[0080] Pick a single colony of the test strain and inoculate it in a 250mL Erlenmeyer flask containing 100mL LB liquid culture, and incubate at 30°C for 48h (count the bacteria under a microscope, and the bacteria density reaches 10 8 cfu / mL or more). Dilute the test bacteria fermentation broth by 1000 times, and use clear water as a control (ck).

[0081] Select tomato seeds with relatively consistent growth and place them in a 9cm transparent culture box with 2-3 layers of filter paper on the bottom, 15 mung bean seeds per culture box, and place them in a 27°C constant temperature artificial climate box with 16 hours of light and 8 hours of darkness. Follow-up and observe the germination of tomatoes, record the number of germination, until no new germination grains appear for 3 consecutive days, measure the length of the germination of the tomato germination radicle machine, and ...

Embodiment 3

[0096] Example 3 Nitrogen fixation of Bacillus

[0097] 1. Measurement method of nitrogen fixation efficiency (National Standard NY411-2000)

[0098] Add 100mL nitrogen-free medium (recipe: 0.2g potassium dihydrogen phosphate, 0.2g magnesium sulfate heptahydrate, 0.2g sodium chloride, 5g calcium sulfate, 10g mannitol, 0.1g calcium sulfate dihydrate, water 1000 mL, pH 7.0), sterilized at 121°C for 30 min. Aseptically, each bottle was connected with two loops of the strain to be tested (or 1 mL of fermentation broth cultured for 3 days), placed on a shaker at 30°C, shaking (120r / min) for 5-7 days, and then taken out to determine the sugar to measure nitrogen. Use anthrone photoelectric colorimetry to determine sugar. Use micro photoelectric colorimetry to measure nitrogen.

[0099] 1.1 Anthrone photoelectric colorimetry

[0100] 1.1.1 Test treatment: Take 1-4mL of fermentation culture broth (depending on the sugar content), dilute to 100mL, take 1.00mL of this diluted solution in a c...

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Abstract

The invention provides rooting-promoting Bacillus sporothermodurans and application thereof, the strain is bacillus tequilensis FJAT-49377 which is preserved in China General Microbiological Culture Collection Center, and the preservation number of the strain is CGMCC NO.16330. The bacillus tequilensis FJAT-49377 can promote seed germination, can dissolve phosphorus and fix nitrogen, can improve soil fertility, provides sufficient nutrition for plant growth, has high temperature resistance, and is beneficial to composting treatment of agricultural wastes.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a Bacillus strain that can promote the growth of phosphorus and nitrogen and fix heat, and its application in plant growth. Background technique [0002] The level of phosphorus supply in the soil is one of the key factors affecting plant growth. 95% of the phosphorus in the soil is in an ineffective form, and it is difficult for plants to directly absorb and utilize it, resulting in the phenomenon of phosphorus deficiency in 74% of the cultivated soil in the country. [0003] In the crop-microbe interaction system, Plant growth-promotingrhizobacteria (PGPR) colonizes the rhizosphere soil of crops, and can effectively decompose the insoluble and fixed elements in the soil (phosphorus, potassium, etc.) , To promote the absorption of fertilizers and elements in the soil by crops, thereby promoting crop growth, increase production and disease resistance. Therefore, the s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P21/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12R2001/07C12N1/205
Inventor 陈峥朱育菁郑梅霞许炼刘波史怀邓文琼李慧敏
Owner 福建省农业科学院农业生物资源研究所
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