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A method for increasing yield and preventing diseases of wheat and its application

A technology of wheat and wheat seeds, applied in the field of crop cultivation, can solve problems such as labor shortage, health threats of beneficial microorganisms, and resistance of pathogenic bacteria, and achieve the effects of improving resistance to stripe rust, increasing chlorophyll content and photosynthetic rate, and increasing thousand-grain weight

Active Publication Date: 2021-07-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The use of chemical agents will serve the purpose of disease control. At the same time, the use of chemical agents will also pose a threat to the survival of beneficial microorganisms and human health. Secondly, long-term and excessive use of chemical agents will easily cause pathogenic bacteria to develop drug resistance. On the one hand, the current agricultural production is facing the social problem of labor shortage

Method used

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  • A method for increasing yield and preventing diseases of wheat and its application
  • A method for increasing yield and preventing diseases of wheat and its application
  • A method for increasing yield and preventing diseases of wheat and its application

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preparation example Construction

[0057] In the present invention, the preparation method of the fermented liquid of described Sclerotinia sclerotiorum comprises: inoculating Sclerotinia sclerotiorum on the liquid fungal culture medium, fermenting at 18~22 ℃, obtains the number of viable bacteria in 1.4×10 5 Fermentation broth of Sclerotinia above cfu / mL.

[0058] In the present invention, the liquid fungal culture medium includes but not limited to PDB medium or broth medium. In the present invention, the fermentation temperature of the sclerotinia is preferably 20°C; the fermentation time is preferably 55-70 hours, more preferably 60-65 hours. In the present invention, the fermentation is accompanied by stirring; more preferably, the stirring speed is preferably 120-200 r / min, more preferably 150 r / min.

[0059] Specifically, the initiation of wheat seeds of the present invention comprises the following steps:

[0060] (1) Soak the wheat seeds to be treated in water after being sterilized, and wait for the...

Embodiment 1

[0068] Embodiment 1: Sclerotinia attenuated bacterial strain DT-8 is to the initiation of wheat seed

[0069] 1. Test material

[0070] Wheat varieties: winter wheat Zheng 9023 and spring wheat Yongliang No. 4, commercially available.

[0071] Sclerotinia attenuated strain DT-8: carrying DNA virus SsHADV-1.

[0072] 2. Preparation of water suspension concentrate of strain DT-8: Cultivate the attenuated strain DT-8 of Sclerotinia sclerotiorum in a 50L fermenter for 60h under the conditions of 20°C, 150r / min, and natural pH. Made by adding 0.05% streptomycin sulfate and 4% trehalose, the number of live bacteria was 1.4×10 5 cfu / mL.

[0073] 3. Initiation of wheat seeds: use 5% 84 disinfectant to sterilize wheat seeds for 10 minutes, wash them with sterile water three times, each time for 1 minute; The attenuated sclerotinia strain DT-8 water suspension was used to mix the wheat seeds at room temperature, soak the seeds for 4 hours, 500mL fermentation broth / 5kg wheat seeds, a...

Embodiment 2

[0074] Embodiment 2: sclerotinia attenuated bacterial strain DT-8 seed causes sclerotinia to grow endogenously in wheat

[0075] 1. Detection of Sclerotinia in wheat seedling leaves by PCR

[0076] The wheat seed Zheng 9023 prepared in Example 1 was cultivated in sterile culture soil for 45 days, the surface of the wheat sample was sterilized (0.5% sodium hypochlorite 10min), rinsed with phosphate buffer (pH7.2) for 3 times after rinsing with distilled water. detection of Sclerotinia in wheat seedlings.

[0077] Genomic DNA extracted from wheat leaves by CTAB method was used as a template, and S. sclerotiorum specific primers XJJ21 and XJJ222 (as shown in Chinese patent ZL200810236958.5, XJJ21: 5'-GTTGCTTTGGCGTGCTGCTC-3' (as shown in SEQ ID NO: 1) ; XJJ222: 5'-CTGACATGGACTCAATAC CAATCTG-3' (shown as SEQ ID NO: 2) is a primer, with Sclerotinia genomic DNA as a positive control, untreated wheat as a negative control, to Sclerotinia in wheat leaves Perform PCR detection. The PC...

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Abstract

The invention provides a method for increasing wheat production and disease prevention and its application, and relates to the field of crop cultivation technology. The method of the invention uses the fermentation liquid of Sclerotinia sclerotiorum to trigger wheat seeds, and the live bacteria of the fermentation liquid of Sclerotinia sclerotiorum Number in 1.4×10 5 cfu / mL or more. The invention utilizes sclerotinia to trigger wheat seeds, and sclerotinia can grow endogenously in wheat plants to induce the expression of wheat disease resistance and development-related genes, thereby achieving the goals of controlling wheat head blight and other diseases and improving wheat yield.

Description

technical field [0001] The invention relates to the technical field of crop cultivation, in particular to a method for increasing wheat production and preventing diseases and its application. Background technique [0002] Wheat is one of the most important food crops in the world, providing about 20% of human food and feeding 40% of the world's population. my country's wheat planting area is about 24 million hectares (Liu Zhiyong et al., 2018), with an output of 1,300 million tons. The total planting area and total output account for about 11% and 17% of the world's respectively. China is one of the most important wheat producing countries in the world. Fungal diseases are a huge threat to wheat production, and scab and rust are common and most harmful. [0003] Wheat scab is caused by fungi such as Fusarium graminearum and F.asiaticum. my country is a "hardest hit area" with a high incidence of wheat scab. Huge economic losses, the average annual yield loss in the middle a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
CPCA01N25/04A01C1/08A01C1/00A01N63/30
Inventor 姜道宏田斌年谢甲涛付艳苹程家森陈桃
Owner HUAZHONG AGRI UNIV
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