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A kind of content determination method of Xiebai Powder

A method of determination, the technology of Xiebai powder, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to fully and accurately reflect the quality of traditional Chinese medicine compositions, and the lack of content determination of various components in Xiebai powder, to achieve Good accuracy, strong specificity, and the effect of improving detection efficiency

Active Publication Date: 2022-04-05
TIANJIN MODERN INNOVATIVE TCM TECH CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the "Chinese Pharmacopoeia" has not stipulated the quality standard of Xiebai powder, and there is no method for simultaneous content determination of multiple components in Xiebai powder in the prior art, which cannot fully and accurately reflect the quality of the traditional Chinese medicine composition

Method used

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  • A kind of content determination method of Xiebai Powder
  • A kind of content determination method of Xiebai Powder
  • A kind of content determination method of Xiebai Powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Adopt high-performance liquid chromatography to simultaneously measure the content of mulberry side A, liquiritin, and ammonium glycyrrhizin in Xiebai powder, and the operation method is as follows:

[0036] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler, 0.1% phosphoric acid solution was used as mobile phase A, acetonitrile was used as mobile phase B, and gradient elution was carried out according to Table 2; the flow rate was 1mL / min, the detection wavelength is 237nm, and the column temperature is 30°C.

[0037] Table 2 Gradient elution program

[0038]

[0039] Preparation of the reference substance solution: Accurately weigh 1 mg of the reference substances of molybdenum A, liquiritin, and ammonium glycyrrhizinate into a 10 mL volumetric flask, add 70% methanol solution to the volume to obtain a reference substance solution with a concentration of 0.1 mg / mL.

[0040] Preparation of the test solution...

Embodiment 2

[0043] Adopt high-performance liquid chromatography to simultaneously measure the content of mulberry side A, liquiritin, and ammonium glycyrrhizin in Xiebai powder, and the operation method is as follows:

[0044] Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel was used as filler, 0.1% phosphoric acid solution was used as mobile phase A, acetonitrile was used as mobile phase B, and gradient elution was carried out according to Table 3; the flow rate was 1mL / min, the detection wavelength of molaritin A is 325nm, the detection wavelength of liquiritin and ammonium glycyrrhizinate is 260nm, and the column temperature is 30°C.

[0045] Table 3 Gradient elution program

[0046]

[0047] Preparation of the reference substance solution: Accurately weigh 1 mg of the reference substances of molybdenum A, liquiritin, and ammonium glycyrrhizinate into a 10 mL volumetric flask, add 70% methanol solution to the volume to obtain a reference sub...

Embodiment 3

[0051] Selection of extraction solvent and its dosage

[0052] Chromatographic conditions: the organic phase is acetonitrile, the aqueous phase is 0.1% phosphoric acid solution, and the gradient elution is carried out according to Table 4; the flow rate is 1mL / min, the detection wavelength is 237nm, the column temperature is 30°C, and the injection volume is 5μL.

[0053] Table 4 Gradient elution program

[0054]

[0055]Plan 1: Take about 0.5g of Xiebai powder and spray dry powder, weigh it accurately, put it into a stoppered Erlenmeyer flask, add 70% ethanol 25mL, 40mL, 50mL, 60mL, 70mL, 80mL, 90mL, 100mL respectively, and seal it tightly. , weighed, sonicated for 30 minutes, allowed to cool, weighed again, supplemented the lost weight with 70% ethanol, shaken well, filtered, took 1 mL of the filtrate in a 1.5 mL EP tube, centrifuged for 10 minutes, and took the supernatant solution as the test solution. Carry out experiment according to above-mentioned chromatographic ...

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Abstract

The invention discloses a content determination method of Xiebai powder, which belongs to the technical field of quality detection of traditional Chinese medicines. High performance liquid chromatography is used to simultaneously measure the contents of moriside A, liquiritin and ammonium glycyrrhizinate in Xiebai powder. By optimizing the chromatographic conditions and the preparation method of the test sample, the simultaneous determination of the contents of molaritin A, liquiritin and ammonium glycyrrhizinate in Xiebai powder was realized. The invention improves the detection efficiency and can reflect the quality of Xiebai powder more comprehensively. The results of the methodological test show that the quality detection method has the advantages of good accuracy, high precision, strong specificity and good stability, which can provide a basis for the formulation of quality standards for Xiebai powder.

Description

technical field [0001] The invention belongs to the technical field of quality detection of traditional Chinese medicines, and relates to a method for determining the content of a traditional Chinese medicine composition, in particular to a method for simultaneously determining the contents of moriside A, liquiritin and ammonium glycyrrhizinate in Xiebai powder by using high performance liquid chromatography . Background technique [0002] Xiebai San, composed of Cortex Mori, Cortex Digu, Licorice, and Japonica Rice, has the effects of clearing lung-heat, relieving cough and relieving asthma. In modern clinics, this prescription is often used to treat children with pneumonia, bronchitis, emphysema or emphysema combined with infection, and children with measles in the early stage, and there are symptoms of coughing, shortness of breath, and persistent body heat. [0003] At present, the "Chinese Pharmacopoeia" has not stipulated the quality standard of Xiebai powder, and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王彦帅郑鑫杰孙明律李想王雪茗丁浩强
Owner TIANJIN MODERN INNOVATIVE TCM TECH CO LTD
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