Detection method for Tibetan medicine semen caesalpiniae cristae

A detection method, the technology of Datuo, applied in the field of medicine, can solve the problems of outdated and backward standard quality control methods, and achieve the effect of simple determination method, accurate result and increased identification

Active Publication Date: 2016-08-31
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the extensive application of preparations such as Liuwei Datuoyeyunshi Powder, the quality control of Datuoyeyunshi is becoming more and more important, but the existing standard quality control methods are outdated and backward

Method used

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  • Detection method for Tibetan medicine semen caesalpiniae cristae
  • Detection method for Tibetan medicine semen caesalpiniae cristae
  • Detection method for Tibetan medicine semen caesalpiniae cristae

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0037] Experimental Example 1: Identification

[0038] Identification method 1: Take 1g of Datuo Yeyunshi medicinal material powder, add 20ml of 6mol / L hydrochloric acid solution, after ultrasonic treatment for 20 minutes, heat and reflux in a water bath for 2 hours, take it out, let it cool, filter, evaporate the filtrate to dryness, add 2ml of 70% ethanol to the residue , dissolved, filtered, and the filtrate was taken as the test solution; another appropriate amount of Datuo Yeyunshi reference drug was taken, and the reference drug solution was prepared in the same way. According to the thin-layer chromatography test, draw 5-10 μl of each of the above two solutions, and spot them on the same silica gel G thin-layer plate, and use n-butanol-glacial acetic acid-water (4:1:1) as the developer to develop. Take it out, dry it, spray it with ninhydrin test solution, and heat it until the spots develop clear color. In the chromatogram of the test product, on the position correspo...

experiment example 2

[0041] Experimental Example 2: Content Determination

[0042] 1. Instruments, reagents and test samples

[0043] Instrument: High Performance Liquid Chromatography: Agilent 1260

[0044] Electronic balance: Shimadzu AUW220D

[0045] UV spectrophotometer: Shimadzu UV﹣2450

[0046] Reference substance: gallic acid reference substance (China Institute for Food and Drug Control), batch number: 110831-200302;

[0047] Catechin reference substance (National Institute for Food and Drug Control), batch number: 110877-201203;

[0048] Sample: Datuo Ye Yunshi (provided by Jinhe Tibetan Medicine Co., Ltd.), batch numbers are: 20160501, 20160502, 20160503

[0049] 2. Detection wavelength selection

[0050] DAD scanning shows that gallic acid has the maximum absorption at 270nm, and catechin has the maximum absorption at 280nm. At the same time, the experiment shows that there are fewer gradient peaks at 280nm, which is more conducive to the determination of samples, so 280nm is selec...

Embodiment 1

[0070] Embodiment 1: a kind of detection method of Tibetan medicine Datuoye Yunshi comprises the following methods

[0071] (1) Identification method one

[0072] Take 2g of Datuoyeyunshi medicinal material powder, add 20ml of 6mol / L hydrochloric acid solution, ultrasonically treat for 20 minutes, heat and reflux in a water bath for 2 hours, take it out, let it cool, filter, evaporate the filtrate to dryness, add 2ml of 70% ethanol to the residue to dissolve, Filtrate, take the filtrate as the test solution; take another appropriate amount of Datuo Yeyunshi reference medicinal material, and prepare the reference medicinal material solution in the same way. According to the thin-layer chromatography test, draw 5 μl of each of the above two solutions, spot them on the same silica gel G thin-layer plate, use n-butanol-glacial acetic acid-water (5:1:1) as the developer, develop, take out, Let it dry, spray it with ninhydrin test solution, and heat until the spots are clear in col...

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Abstract

The invention discloses a detection method for Tibetan medicine semen caesalpiniae cristae. The detection method comprises the steps that identifying is conducted by adopting thin layer chromatography, and the content is detected by adopting high performance liquid chromatography. According to the method, the standard of existing Tibetan medicine semen caesalpiniae cristae is correspondingly improved, identification and content detection are increased, the quality of semen caesalpiniae cristae is more comprehensively reflected, therefore, the quality of the medicinal material is overall described and evaluated, and the safety, the effectiveness and the quality controllability of the medicinal material are guaranteed. Meanwhile, quantitative analysis of multi-components by single marker is adopted, samples with the large polarity difference are detected in a same mobile phase, the determination method is simpler, and a result is more accurate.

Description

technical field [0001] The invention relates to a method for detecting Tibetan medicine Datuoye Yunshi, which belongs to the technical field of medicine. Background technique [0002] Datuoyeyunshi is the dry mature seed of the leguminous plant Caesalpinia crista L., which is commonly used by Tibetans. Warming the kidney, expelling cold, for kidney cold disease, stomach cold. The original quality standard is recorded in the standard (Tibetan medicine part) issued by the Ministry of Health of the People's Republic of China, standard number: WS3-BC-0003-95, and the standard only has physical and chemical identification. With the extensive application of Liuwei Datuoyeyunshi powder and other preparations, the quality control of Datuoyeyunshi is becoming more and more important, but the existing standard quality control methods are outdated and backward. Contents of the invention [0003] Aiming at the deficiencies of the current situation, the present invention establishes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 孙绪丁李怀平姬涛
Owner SHANDONG JINHE DRUG RES DEV
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