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Chitosan/silk fibroin/chitosan microsphere-ciprofloxacin (CS/SF/CMs) anti-microbial composite scaffold for promoting seawater immersion wound healing and preparation method for anti-microbial composite scaffold

A wound healing and composite stent technology, applied in the field of biomedical engineering materials, can solve problems such as organ damage, drug burst release, inflammatory response, etc., and achieve the effects of preventing wound deterioration, avoiding burst release, and reducing infiltration.

Active Publication Date: 2020-05-15
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical combination of the carrier and the antibiotic can play an antibacterial effect, but it is easy to cause the explosive release of the drug in the body and trigger an inflammatory response, causing organ damage

Method used

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  • Chitosan/silk fibroin/chitosan microsphere-ciprofloxacin (CS/SF/CMs) anti-microbial composite scaffold for promoting seawater immersion wound healing and preparation method for anti-microbial composite scaffold
  • Chitosan/silk fibroin/chitosan microsphere-ciprofloxacin (CS/SF/CMs) anti-microbial composite scaffold for promoting seawater immersion wound healing and preparation method for anti-microbial composite scaffold
  • Chitosan/silk fibroin/chitosan microsphere-ciprofloxacin (CS/SF/CMs) anti-microbial composite scaffold for promoting seawater immersion wound healing and preparation method for anti-microbial composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] The embodiment of the present invention provides a method for preparing an antibacterial composite scaffold CS / SF / CMs-CIP that promotes seawater soaked wound healing, and the specific steps are as follows:

[0043] (1) taking by weighing 2g chitosan and being dissolved in 100mL mass fraction is 1% in the acetic acid aqueous solution, continuous stirring makes it dissolve completely, obtains chitosan solution;

[0044] (2) Pour 4.5-5.5 g of sodium bicarbonate, an appropriate amount of sodium lauryl sulfate, and an appropriate amount of shredded cocoons into 1L of deionized water, and degumming in a boiling water bath for 50-70 minutes; weigh 1.8-2.5 g of the washed and dried cocoons. g is dissolved in 0.5L calcium chloride / ethanol aqueous solution to prepare silk fibroin solution; the calcium chloride / ethanol aqueous solution is configured by water, absolute ethanol, and anhydrous calcium chloride with a molar ratio of 8:2:1; After dialyzing the silk fibroin solution, ce...

Embodiment 1～7

[0055] The experimental method that embodiment 1~7 adopts:

[0056] (1) water absorption

[0057] Weigh the weight of the stent with a balance, record it as Mo, place the stent in PBS (10mmol / L, pH=7.4) solution, quickly absorb the surface moisture with filter paper after 30min, weigh and record the sample mass as Mw. Each sample was tested three times in parallel, and the average value was calculated. The water absorption rate X of the sample was calculated according to the following formula:

[0058]

[0059] Among them, M o is the weight of the dry support (g); M w is the weight (g) of the stent after absorbing water; X is the water absorption rate (%) of the stent.

[0060] (2) Porosity

[0061] Measure the porosity of the sample with the medium saturation method, weigh the weight of the support with a balance, and record it as W 1 , soak the bracket in absolute ethanol until the sample is saturated, attach filter paper on both sides to absorb a certain pressure fo...

Embodiment 1

[0075] Embodiment 1: the preparation of chitosan microspheres (CMs)

[0076] (1) take by weighing 1g chitosan, be dissolved in 10mL mass fraction and be in the acetic acid aqueous solution of 3%, prepare chitosan solution;

[0077] (2) Add a certain amount of liquid paraffin and 2g Span-20 into the above-mentioned chitosan solution, and emulsify at 500r / min for 90min under nitrogen protection;

[0078] (3) Under stirring conditions, add 14 mL of formaldehyde with a mass fraction of 3.7% to the above emulsion, and react for 1 hour, then add a certain amount of formaldehyde with a mass fraction of 37%, and react for 1 hour;

[0079] (4) After the reaction is over, pass through ammonia gas to solidify for 25 minutes, then stir in a water bath at 55°C for 30 minutes;

[0080] (5) After the reaction is over, use sherwood oil, acetone and absolute ethanol to ultrasonically wash several times in sequence, and finally the product in absolute ethanol is centrifuged;

[0081] (6) Soak...

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Abstract

The invention discloses a preparation method for a chitosan / silk fibroin / chitosan microsphere-ciprofloxacin (CS / SF / CMs) anti-microbial composite scaffold for promoting seawater immersion wound healing. The method comprises the specific steps that a chitosan solution and a silk fibroin solution are mixed, ciprofloxacin-encapsulated chitosan drug-loaded microspheres CMs-CIP are added, uniform mixingis performed, a cross-linking agent is added, and freeze drying is performed after overnight cold storage to obtain the CS / SF / CMs anti-microbial composite scaffold. The anti-microbial composite scaffold is a superior material for treating a seawater immersion wound; the anti-microbial composite scaffold is of a uniform porous net structure, can absorb wound exudates to keep a suitable moisture environment for the wound, and increases contact area between the wound and outside oxygen to promote wound healing; the anti-microbial composite scaffold can also slowly release CIP to avoid side effects caused by burst release; and the released CIP has a broad-spectrum antibacterial property, and can resist wound infections.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering materials, in particular to an antibacterial composite scaffold CS / SF / CMs-CIP for promoting wound healing soaked in seawater and a preparation method thereof. Background technique [0002] As the largest organ of the human body, the skin can resist ultraviolet radiation, chemical corrosion and foreign biological invasion. However, once the skin suffers from trauma and burns, it will often lead to tissue necrosis, destroying the defense function, coupled with wound infection, which will not only hinder wound healing but also make the wound worse. After sea workers are injured, their wounds are often soaked in seawater. Due to the low temperature of seawater, high sodium content, high osmotic pressure, and a large number of microorganisms, the wound will be aggravated. Severe wound infection is associated with the development of bacteremia and sepsis, and may even lead to a systemic ...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/50A61L27/56A61L27/54A61L27/22A61L27/20
CPCA61L27/20A61L27/227A61L27/50A61L27/54A61L27/56A61L27/60A61L2300/406A61L2300/602C08L89/00C08L5/08
Inventor 王欣璐程飚侯鹏
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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