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Application of Parabacteroides distasonis in prevention or treatment of obesity or related diseases thereof

A parabacteroides, obesity technology, applied in the field of microorganisms, can solve problems such as the limitation of disease problems, and achieve the effect of increasing abundance and alleviating symptoms of obesity and related diseases

Active Publication Date: 2020-02-28
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the intestinal microorganisms currently used to prevent or treat diseases are concentrated in a few species such as Bifidobacteria and Lactobacillus, so the disease problems that can be solved by the currently discovered microbial populations are relatively limited

Method used

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  • Application of Parabacteroides distasonis in prevention or treatment of obesity or related diseases thereof
  • Application of Parabacteroides distasonis in prevention or treatment of obesity or related diseases thereof
  • Application of Parabacteroides distasonis in prevention or treatment of obesity or related diseases thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The separation and purification of embodiment 1 Parabacteroides gizzardii

[0047] Separation and purification method

[0048] (1) Preparation of sterile preservation solution: Weigh 30 g of polyethylene glycol (molecular weight 3350) and dissolve it in 1000 mL of normal saline, and autoclave at 121° C. for 15 minutes to obtain a sterile preservation solution for later use.

[0049](2) Take an appropriate amount of fresh feces samples from healthy yellow people and place them in a sample preservation tube containing sterile preservation solution, mix them with a vortex oscillator for 10 minutes, and then dilute them to 10% with sterile saline in an anaerobic workstation. -6 Dilution: Draw 0.1mL of the bacterial solution and spread it on an anaerobic blood agar plate, and incubate in an anaerobic workstation at 37°C for 72 hours. Using the partition marking method, a sterile toothpick was used to pick single colonies of different shapes and place them on the anaerobic b...

Embodiment 2

[0052] Embodiment 2 Effect of Parabacteroides gibsonii on body weight, blood sugar, blood lipid and liver function of obese mice induced by high-fat diet

[0053] Weight loss drug: liraglutide, trade name -Novo Nordisk, subcutaneous injection at 150μg / kg / d.

[0054] experimental method:

[0055] (1) Use sterile PBS to adjust the concentration of the cultivated Parabacteroides gibsonii to 1×10 9 CFU / mL, the volume of gavage is 0.1mL, and gavage once a day.

[0056] (2) Select 6-8 week old male C57BL / 6 type mice and divide them into four groups randomly, each group is set up with 9 parallel groups, and each group is allocated and set as follows,

[0057] Control group 1: normal maintenance feed + sterile saline;

[0058] Control group 2: high-fat feed + sterile saline;

[0059] Control group 3: high-fat feed + weight-loss drugs;

[0060] Experimental group 4: high-fat diet + Parabacteroides gibsonii.

[0061] Among them, the normal maintenance feed has 12% fat calories, ...

Embodiment 3

[0085] Example 3 Regulating effect of Parabacteroides gibsonii on intestinal flora

[0086] experimental method:

[0087] (1) Use sterile PBS to adjust the concentration of the cultivated Parabacteroides gibsonii to 1×10 9 CFU / mL, the volume of gavage is 0.1mL, and gavage once a day.

[0088] (2) 6-8 week-old male C57BL / 6 mice were fed high-fat diet and intragastrically administered Parabacteroides gibsonii for 6 consecutive weeks. At week 0 (before the experiment) and week 6 (after the experiment ) to collect mouse feces, extract the bacterial genome DNA in the feces, and determine the changes in the intestinal flora of the mice through 16s DNA sequencing analysis.

[0089] Table 7: Effect of Parabacteroides gibsonii on the relative abundance of intestinal flora at the genus level

[0090] time Bacteroides Parabacteroides Lactobacillus week 0 0.035 0.000 5.083 week 6 1.020 0.800 8.260

[0091] Depend on figure 1The results showed that ...

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Abstract

The invention relates to application of Parabacteroides distasonis in the prevention or treatment of obesity or related diseases thereof. The Parabacteroides distasonis can effectively relieve the obesity and related diseases such as hyperlipidemia, diabetes, abnormal liver function and abnormal intestinal microecology, can increase the abundance of intestinal beneficial microbial flora and applicable to drugs, food or healthcare products for preventing and treating the obesity and related diseases.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to the application of Parabacteroides gibsonii in the prevention or treatment of obesity or related diseases. Background technique [0002] Obesity is a complex chronic disease. It is a chronic metabolic disease caused by the interaction of multiple factors including genetic and environmental factors. Its pathogenesis is still unclear. Obesity also increases the risk of a series of diseases, such as hypertension, diabetes, hyperlipidemia, coronary heart disease and cancer. According to the data of the World Health Organization, the current global obesity incidence rate has exceeded 14%, but this figure is still rising, and obesity has become an important problem that threatens human health. [0003] At present, the main means of treating obesity is drug therapy. Common weight-loss drugs include orlistat, liraglutide, sibutramine, and naltrexone. absorption etc. However, the ...

Claims

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Application Information

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IPC IPC(8): A23C9/12A23C9/152A23C17/02A23L2/38A23L9/10A23L33/135C12N1/20A61K35/741A61P3/04A61P3/06A61P3/10A61P1/16A61P1/00C12R1/01
CPCA23L33/135A23C9/1203A23C17/02A23C9/152A23L2/38A23L9/10A61K35/741A61P3/04A61P3/06A61P3/10A61P1/16A61P1/00C12R2001/01C12N1/205
Inventor 蔡文娇黄宝家孔萍肖晨赵莹莹蒋先芝
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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