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Combined porcine transmissible gastroenteritis and porcine epizootic diarrhea living vaccine cryoprotectant and combined living vaccine

A technology for porcine epidemic diarrhea and freeze-dried protective agent, which is used in vaccines, freeze-dried transportation, veterinary vaccines, etc., can solve the problems of slow dissolving speed of freeze-dried products, depressions on the surface, loss of titer, etc., and reduce the drug price. loss, smooth surface, improved protective effect

Active Publication Date: 2020-02-18
SICHUAN HUASHEN ANIMAL BIOLOGICAL PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a lyoprotectant for porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccine and a dual live vaccine, which can effectively solve the problem of existing freeze-dried products There are depressions and cracks on the surface, the slow dissolution rate of freeze-dried products and the loss of potency after freeze-drying

Method used

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  • Combined porcine transmissible gastroenteritis and porcine epizootic diarrhea living vaccine cryoprotectant and combined living vaccine
  • Combined porcine transmissible gastroenteritis and porcine epizootic diarrhea living vaccine cryoprotectant and combined living vaccine

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Effect test

Embodiment 1

[0017] A freeze-dried protective agent for porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccine, which is composed of the following components in mass and volume percentages: 20% sucrose, 6% gelatin, 2% tryptone, 1% yeast extract powder, Chondroitin sulfate 0.1% and inositol 1%, the balance is water for injection.

[0018] A dual live vaccine, comprising a mixed virus liquid containing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus and the above-mentioned porcine transmissible gastroenteritis, porcine epidemic diarrhea dual live vaccine lyoprotectant, wherein, The volume ratio of the mixed virus solution to the lyoprotectant is 4:1.

[0019] The preparation process of the above-mentioned dual live vaccine is as follows: (1) mixing and dissolving each component in the lyoprotectant, and then performing sterilization treatment; (2) mixing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea vi...

Embodiment 2

[0021] A freeze-dried protective agent for porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccine, which consists of the following components in mass and volume percentages: 28% sucrose, 10% gelatin, 6% tryptone, 3% yeast extract powder, Chondroitin sulfate 2% and inositol 5%, the balance is water for injection.

[0022] A dual live vaccine, comprising a mixed virus liquid containing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus and the above-mentioned porcine transmissible gastroenteritis, porcine epidemic diarrhea dual live vaccine lyoprotectant, wherein, The volume ratio of the mixed virus solution to the lyoprotectant is 8:1.

[0023] The preparation process of the above-mentioned dual live vaccine is as follows: (1) mixing and dissolving each component in the lyoprotectant, and then performing sterilization treatment; (2) mixing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus ...

Embodiment 3

[0025] A freeze-dried protective agent for porcine transmissible gastroenteritis and porcine epidemic diarrhea dual live vaccine, which is composed of the following components in mass and volume percentages: 24% sucrose, 8% gelatin, 4% tryptone, 2% yeast extract powder, Chondroitin sulfate 1.5% and inositol 3%, the balance is water for injection.

[0026] A dual live vaccine, comprising a mixed virus liquid containing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea virus and the above-mentioned porcine transmissible gastroenteritis, porcine epidemic diarrhea dual live vaccine lyoprotectant, wherein, The volume ratio of the mixed virus solution to the lyoprotectant is 7:1.

[0027] The preparation process of the above-mentioned dual live vaccine is as follows: (1) mixing and dissolving each component in the lyoprotectant, and then performing sterilization treatment; (2) mixing porcine transmissible gastroenteritis virus and porcine epidemic diarrhea vi...

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Abstract

The invention provides a combined porcine transmissible gastroenteritis and porcine epizootic diarrhea living vaccine cryoprotectant and a combined living vaccine. The cryoprotectant is prepared fromthe following components in percentage by mass volume: 20-28 percent of cane sugar, 6-10 percent of gelatin, 2-6 percent of tryptone, 1-3 percent of yeast extract powder, 0.1-2 percent of chondroitinsulfate, 1-5 percent of inositol and the balance being injection water, based on the unit of mass volume ratio being g / ml. The combined living vaccine comprises the cryoprotectant, the cryoprotectantcan effectively solve the problem that the surface of an existing lyophilized product has pits and cracks, and the lyophilized product has low dissolving speed and titer loss after lyophilization.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a freeze-drying protectant for a dual live vaccine against porcine transmissible gastroenteritis and porcine epidemic diarrhea and the dual live vaccine. Background technique [0002] Freeze-dried vaccines are mainly composed of antigens and freeze-dried protective agents. Antigens can include proteins, viruses, bacteria, etc. At present, the market application of veterinary freeze-dried vaccines is relatively common. Although freeze-dried vaccines have the advantages of convenient storage and centralized mass production, there are still some problems in the freeze-drying process or freeze-drying effect, which restricts the development of freeze-dried vaccines. The inactivation of virus particles or bacteria during antigen preservation and vaccine freeze-drying is one of the important factors restricting the quality of vaccines. [0003] In order to maintain the im...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K47/26A61K47/42A61K47/46A61K47/10A61K39/295A61K39/225A61K39/215A61P31/14A61P1/12
CPCA61K9/19A61K39/12A61K47/10A61K47/26A61K47/42A61K47/46A61K2039/552A61K2039/70A61P1/12A61P31/14C12N2770/20034
Inventor 张传明肖昌建邝声耀阴文奇周远成郭沛王子健
Owner SICHUAN HUASHEN ANIMAL BIOLOGICAL PRODS
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