A kind of preparation method of eggshell membrane polypeptide

An eggshell membrane and shell membrane separation technology, applied in the food field, can solve the problems of product separation and purification, waste water treatment burden, poor water solubility of keratin, potential safety hazards, etc., achieve good antioxidant activity, product safety, and avoid toxic and harmful reagents. Effect

Active Publication Date: 2021-10-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor water solubility of keratin, there are few active sites for enzyme interaction, resulting in long reaction time and low enzymatic hydrolysis efficiency
Therefore, the eggshell membrane can be pretreated to improve the efficiency of enzymatic hydrolysis, such as the use of strong alkali, ionic liquid, organic reagents, etc., but these pretreatment methods will bring burdens to product separation and purification and wastewater treatment and have potential safety hazards

Method used

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  • A kind of preparation method of eggshell membrane polypeptide
  • A kind of preparation method of eggshell membrane polypeptide
  • A kind of preparation method of eggshell membrane polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Preparation of raw materials: Wash and sterilize the collected eggshells, separate the shell membranes through a shell membrane separator, dry the obtained eggshell membranes at 50°C for 8 hours, then pulverize them, and pass through a 100-mesh sieve to obtain eggshell membranes. Shell powder.

[0046] (2) Pretreatment: adjust the water content of the eggshell membrane powder obtained in step (1) to be 20%, control the screw speed to be 100r / min, carry out extrusion and puffing treatment at a temperature of 140°C, and obtain the eggshell After the puffed film is crushed and passed through a 60-mesh sieve, it is treated with a dilute alkali solution. The eggshell membrane powder that has been extruded, crushed and sieved is mixed with 0.1M NaOH at a temperature of 30°C at a solid-to-liquid ratio of 1:15 (w / w), and stirred at a constant temperature for 3 hours to obtain a mixture.

[0047] (3) Enzymolysis: adjust the temperature of the mixture obtained in step (2) to...

Embodiment 2

[0054] (1) Preparation of raw materials: Wash and sterilize the collected eggshells, separate the shell membranes through a shell membrane separator, dry the obtained eggshell membranes at 50°C for 6 hours, and then crush them to eggshell membranes with a particle size of 100 mesh powder.

[0055] (2) Pretreatment: adjust the water content of the eggshell membrane powder obtained in step (1) to be 20%, control the screw speed to be 100r / min, carry out extrusion and puffing treatment at a temperature of 140°C, and obtain the eggshell After the puffed film is crushed and passed through a 60-mesh sieve, it is treated with a dilute alkali solution. The eggshell membrane powder after extrusion, crushing and sieving was mixed with 0.25M NaOH at a temperature of 30°C at a solid-to-liquid ratio of 1:15 (w / w), and stirred at a constant temperature for 6 hours to obtain a mixture.

[0056] (3) Enzymolysis: adjust the temperature of the mixture obtained in step (2) to 55° C., and add al...

Embodiment 3

[0063] (1) Preparation of raw materials: Wash and sterilize the collected eggshells, separate the shell membranes through a shell membrane separator, dry the obtained eggshell membranes at 55°C for 8 hours, then pulverize them, and pass through a 100-mesh sieve to obtain eggshell membranes. Shell powder.

[0064] (2) Pretreatment: adjust the water content of the eggshell membrane powder obtained in step (1) to 20%, control the screw speed to 100r / min, carry out extrusion and puffing treatment at a temperature of 140°C, and obtain the eggshell After the puffed film is crushed and passed through a 60-mesh sieve, it is treated with a dilute alkali solution. The eggshell membrane powder that has been extruded, crushed and sieved is mixed with 0.1M NaOH at a temperature of 30°C at a solid-to-liquid ratio of 1:15 (w / w), and stirred at a constant temperature for 3 hours to obtain a mixture.

[0065] (3) Enzymolysis: adjust the temperature of the mixture obtained in step (2) to 55° C...

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Abstract

The invention discloses a preparation method of eggshell membrane polypeptide, which belongs to the field of food. The present invention adopts a combination technology of extrusion, dilute alkali and enzymatic hydrolysis, that is, firstly extruding and dilute alkali pretreatment is performed on the eggshell membrane, and then enzymatic hydrolysis, in order to improve the efficiency of enzymatic hydrolysis and avoid the use of ionic liquids , organic solvents and other toxic reagents, thereby preparing shell membrane polypeptides with antioxidant properties, and rich in mucopolysaccharides, the yield of mucopolysaccharides reaches 23.58mg / g eggshell membrane, and the recovery rate of total nitrogen reaches 78.09%. The product eggshell membrane polypeptide has good antioxidant activity, and its molecular weight is mainly below 1000Da, accounting for 89.54%.

Description

technical field [0001] The invention relates to a preparation method of eggshell membrane polypeptide, which belongs to the field of food. Background technique [0002] The main component of the eggshell membrane is protein, accounting for about 90% of the total membrane weight, mainly in the form of glycoprotein, and also contains about 3% liposome and 2% mucopolysaccharides. The protein is mainly keratin, and also contains collagen, lysozyme, ovotransferrin, ovalbumin, etc., and the variety is very rich. Mucopolysaccharides are mainly hyaluronic acid and chondroitin sulfate. Among them, hyaluronic acid is known as the most ideal natural moisturizing factor, which has excellent moisturizing effect and can resist skin aging and wrinkles. Chondroitin sulfate has physiological functions such as regulating metabolism, anti-inflammation, anti-virus, and anti-tumor, and has been used in the clinical treatment of rheumatism, nephritis, peptic ulcer and other diseases. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K14/465C07K1/14C07K1/34C08B37/00
CPCC07K1/145C07K1/34C07K14/465C07K14/78C08B37/0003C12P21/06Y02P20/54
Inventor 苏宇杰张钰顾璐萍杨严俊常翠华李俊华
Owner JIANGNAN UNIV
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