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Triple inactivated vaccine and preparation method thereof

An inactivated vaccine and inactivated technology, applied in the field of vaccines, can solve problems such as loss of avian adenovirus breeding industry, and achieve the effects of good protection, low formaldehyde and endotoxin content, and strong immunogenicity

Inactive Publication Date: 2020-01-14
洛阳职业技术学院 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there is no clinically approved poultry adenovirus vaccine at present, the prevalence of poultry adenovirus brings huge losses to the breeding industry

Method used

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  • Triple inactivated vaccine and preparation method thereof
  • Triple inactivated vaccine and preparation method thereof
  • Triple inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The triple inactivated vaccine of the present embodiment, the antigen of the triple inactivated vaccine is the inactivated Newcastle disease La Sota virus strain, the inactivated H9 subtype avian influenza QF01 strain and the inactivated I group 8b avian adenovirus Hexon protein The amino acid sequence of the I group 8b type avian adenovirus Hexon protein is shown in SEQ ID NO.2; the nucleotide of the I group 8b type avian adenovirus Hexon protein is shown in SEQ ID NO.1; Chicken Newcastle disease La Sota virus strain content ≥ 10 8.5 EID 50 / 0.1mL; the content of the H9 subtype avian influenza QF01 strain ≥ 10 7.5 EID 50 / 0.1mL; the agar amplification titer of described I group 8b type avian adenovirus Hexon protein content ≥ 1:32;

[0040] The chicken Newcastle disease La Sota virus strain is derived from China Veterinary Medicine Supervision Institute;

[0041] The H9 subtype avian influenza QF01 strain belongs to influenza virus and was preserved in the General ...

Embodiment 2

[0078] Prepare the triple inactivated vaccine of three batches according to the preparation method of the triple inactivated vaccine of embodiment 1, batch number is respectively NAFs01, NAFs02 and NAFs03, carry out safety test respectively:

[0079] (1) Single-dose one-time vaccination safety test

[0080] Get 10 days old SPF (no specific pathogen) chicken, the injection dosage of described triple inactivated vaccine is 0.3mL / , 10 / group, totally three groups, the 10 days old SPF chicken injection batch number of the first group is NAFs01 Triple inactivated vaccine, the 10-day-old SPF chicken injection batch number of the second group is the triple inactivated vaccine of NAFs02, and the 10-day-old SPF chicken injection batch number of the third group is the triple inactivated vaccine of NAFs03; The 10-day-old SPF chicken of the inactivated vaccine is a negative control group, and the injection dose of normal saline injected into the neck of the 10-day-old SPF chicken of the ne...

Embodiment 3

[0116] Be respectively the triple inactivated vaccine of three batches of NAFs01, NAFs02 and NAFs03 with the batch number among the embodiment 2, carry out immune efficacy test respectively:

[0117] (1) Efficacy experiment of chicken Newcastle disease virus

[0118] The efficiency experiment of chicken Newcastle disease virus was carried out by serological method and immune challenge method respectively;

[0119] (1) Serological method: get 21 day old SPF chickens, the immunization dosage of the subcutaneous inoculation of described triple inactivated vaccine is 20 μ L / , 10 / group, altogether three groups, described three groups are immunization groups, the first One group of 21-day-old SPF chickens was subcutaneously inoculated with the triple inactivated vaccine with batch number NAFs01; The triple inactivated vaccine of NAFs03; Establish simultaneously 5 21-day-old SPF chickens without injecting triple-inactivated vaccines as negative control group, and the 21-day-old SPF ...

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Abstract

The invention provides a triple vaccine. Antigens of the triple inactivated vaccine include an inactivated chicken newcastle disease La Sota virus strain, an inactivated H9 subtype avian influenza QF01 strain and an inactivated I-group 8b type fowl adenovirus Hexon protein. The invention further provides a preparation method of the triple inactivated vaccine. The preparation method comprises the following steps of enabling the I-group 8b type fowl adenovirus Hexon protein to be inactivated through pyrrole, and performing mixing and emulsifying on the inactivated I-group 8b type fowl adenovirusHexon protein, an inactivated newcastle disease virus concentrated solution and an inactivated avian influenza virus concentrated solution to obtain the triple inactivated vaccine. The I-group 8b type fowl adenovirus Hexon protein inactivated by the triple inactivated vaccine is high in content, high in immunogenicity and low in formaldehyde content and endotoxin content, has better protection effects on current epidemic I-group 8b type fowl adenovirus, is good in safety properties and true in immune effects, can resist infection of clinical chicken groups on newcastle disease virusese, avianinfluenza and I-group 8b type fowl adenovirus, and is long in persistent period, the immunity persistent period can reach 5 months under the condition that 0.3mL of the vaccine is only used once, andat least 70% of the protection ratio can be provided for the chicken groups.

Description

technical field [0001] The invention belongs to the technical field of vaccines, in particular to a triple inactivated vaccine and a preparation method thereof. Background technique [0002] Newcastle disease (ND) is an acute, febrile, septic and highly contagious infectious disease of poultry caused by Newcastle disease virus. It is characterized by high fever, dyspnea, diarrhea, nervous disturbance, and mucosal and serosal hemorrhage. It has a high morbidity and fatality rate, and is one of the most serious severe infectious diseases that endanger the global poultry industry. OIE lists it as a Class A epidemic disease, and it is one of the five first-class animal diseases stipulated in my country's "National Medium and Long-term Animal Disease Prevention and Control Plan" for priority prevention and control. It is listed as a notifiable animal disease by the World Organization for Animal Health (OIE). [0003] Avian influenza (Avian influenza, AI) is a kind of infectiou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/235A61K39/145A61K39/17A61P31/20A61P31/14A61P31/16C07K14/075C12N15/866
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70A61P31/14A61P31/16A61P31/20C07K14/005C12N15/86C12N2710/10222C12N2710/10234C12N2710/14043C12N2760/16134
Inventor 张贺伟杨振董均杨少宗李琛宋敏杰田文静郭少阳张淼丹程相朝张春杰
Owner 洛阳职业技术学院
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