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A kind of triple inactivated vaccine and preparation method thereof

An inactivated vaccine and inactivation technology, applied in the field of vaccines, can solve problems such as loss of avian adenovirus breeding industry, and achieve the effects of good protection effect, low formaldehyde and endotoxin content, and reliable immune effect.

Inactive Publication Date: 2021-11-26
洛阳职业技术学院 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there is no clinically approved poultry adenovirus vaccine at present, the prevalence of poultry adenovirus brings huge losses to the breeding industry

Method used

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  • A kind of triple inactivated vaccine and preparation method thereof
  • A kind of triple inactivated vaccine and preparation method thereof
  • A kind of triple inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The triple inactivated vaccine of the present embodiment, the antigens of the triple inactivated vaccine are inactivated chicken Newcastle disease La Sota virus strain, inactivated H9 subtype avian influenza QF01 strain and inactivated I group 8b type avian adenovirus Hexon protein ; The amino acid sequence of the I group 8b type avian adenovirus Hexon protein is shown in SEQ ID NO.2; the nucleotide of the I group 8b type avian adenovirus Hexon protein is shown in SEQ ID NO.1; the described The content of Newcastle disease La Sota virus strain in chickens ≥ 10 8.5 EID 50 / 0.1mL; the content of the H9 subtype avian influenza QF01 strain ≥ 10 7.5 EID 50 / 0.1mL; The agar expansion titer of described I group 8b type avian adenovirus Hexon protein content ≥ 1:32;

[0040] Described chicken Newcastle disease La Sota virus strain comes from China Veterinary Drug Administration;

[0041] The H9 subtype avian influenza QF01 strain belongs to influenza virus and was deposited...

Embodiment 2

[0078] Three batches of triple inactivated vaccine were prepared according to the preparation method of the triple inactivated vaccine of Example 1, and the batch numbers were NAFs01, NAFs02 and NAFs03 respectively, and safety tests were carried out respectively:

[0079] (1) Safety test of single-dose one-time vaccination

[0080] Take 10-day-old SPF (no specific pathogens) chickens, the injection dose of the triple inactivated vaccine is 0.3 mL / piece, 10 / group, there are three groups in total, and the 10-day-old SPF chickens in the first group are injected with a batch number of NAFs01. Triple inactivated vaccine, 10-day-old SPF chickens in the second group were injected with triple inactivated vaccine with batch number NAFs02, and 10-day-old SPF chickens in the third group were injected with triple inactivated vaccine with batch number NAFs03; at the same time, 10 uninjected triple inactivated vaccines were set. The 10-day-old SPF chickens with the inactivated vaccine were ...

Embodiment 3

[0116] The three batches of triple inactivated vaccines with the batch numbers of NAFs01, NAFs02 and NAFs03 in Example 2 were respectively carried out immune efficacy test:

[0117] (1) Efficacy experiment of chicken Newcastle disease virus

[0118] The efficacy experiment of chicken Newcastle disease virus was carried out by serological method and immune challenge method respectively;

[0119] (1) Serological method: 21-day-old SPF chickens were taken. The subcutaneously inoculated immunization dose of the triple inactivated vaccine was 20 μL / bird, 10 birds / group, and there were three groups in total. The three groups were all immune groups. The 21-day-old SPF chickens in one group were subcutaneously inoculated with the triple inactivated vaccine batch number NAFs01, the SPF chickens in the second group were subcutaneously inoculated with the triple inactivated vaccine batch number NAFs02 on the 21st day, and the SPF chickens in the third group were subcutaneously inoculated...

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Abstract

The invention provides a triple vaccine, wherein the antigens of the triple inactivated vaccine are inactivated Newcastle disease La Sota virus strain, inactivated H9 subtype avian influenza QF01 strain and inactivated I group 8b avian adenovirus Hexon protein , and also provides its preparation method: after the Hexon protein of group I 8b avian adenovirus is inactivated by diethyleneimine, it is mixed with inactivated chicken Newcastle disease virus concentrate and inactivated avian influenza virus concentrate to obtain a triple inactivated vaccine after emulsification . The inactivated I group 8b avian adenovirus of the triple inactivated vaccine of the present invention has high Hexon protein content, strong immunogenicity, low formaldehyde and endotoxin content, and has better protection for the currently popular I group 8b avian adenovirus Efficacy, good safety performance, reliable immune effect, can resist the infection of Newcastle disease virus, avian influenza virus and group I 8b avian adenovirus to clinical chickens, and the duration is long. When only 0.3mL vaccine is used once, the immunity lasts The period can reach five months, providing at least 70% protection rate to the flock.

Description

technical field [0001] The invention belongs to the technical field of vaccines, and in particular relates to a triple inactivated vaccine and a preparation method thereof. Background technique [0002] Newcastle disease (ND) is an acute, febrile, septicemic and highly contagious infectious disease of poultry caused by Newcastle disease virus. Characterized by high fever, dyspnea, diarrhea, neurological disturbances, and mucosal and serous hemorrhages. With high morbidity and mortality, it is one of the most serious infectious diseases that endanger the global poultry industry. It is listed as a Category A disease by OIE, and it is one of the five first-class animal diseases specified in the National Medium- and Long-Term Animal Disease Prevention and Control Plan for priority control and prevention. It is listed as a notifiable animal disease by the World Organization for Animal Health (OIE). [0003] Avian influenza (AI) is a kind of infectious disease caused by Avian i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/235A61K39/145A61K39/17A61P31/20A61P31/14A61P31/16C07K14/075C12N15/866
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70A61P31/14A61P31/16A61P31/20C07K14/005C12N15/86C12N2710/10222C12N2710/10234C12N2710/14043C12N2760/16134
Inventor 张贺伟杨振董均杨少宗李琛宋敏杰田文静郭少阳张淼丹程相朝张春杰
Owner 洛阳职业技术学院
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