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Method for culturing female virus-free seedlings of elaeagnus angustifolia

A cultivation method, the technology of Lavender, which is applied in the field of plant cultivation, can solve the problems of the shortage of Lavender and the low survival rate, and achieve the effects of alleviating the contradiction between supply and demand of medicinal materials, fast growth, and improving quality

Active Publication Date: 2019-10-25
湖北金水源农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of the shortage of golden fruit olive resources and the low survival rate caused by the uneradicated virus existing in the existing artificial seedling breeding technology, and to provide a method for cultivating virus-free seedlings of golden fruit olive female plants

Method used

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  • Method for culturing female virus-free seedlings of elaeagnus angustifolia
  • Method for culturing female virus-free seedlings of elaeagnus angustifolia
  • Method for culturing female virus-free seedlings of elaeagnus angustifolia

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A method for cultivating virus-free seedlings of olive oleracea female plants, comprising the following steps:

[0042] 1) Selection of female plants of L. aurea: female plants of L. aurea in the wild state, with normal flowering and fruiting, strong plants, and no pests and diseases 2-3 years before collection, are selected as the material collection objects; the collection time is selected at From March to June every year, before the plant blooms, select the vigorously growing stem tip of the main stem or side stem as the explant;

[0043]2) Disinfection of the surface of the stem tip of the female Lavender plant: cut the collected stem tip to 1-1.5 cm, add detergent to the tap water to clean the dirt attached to the surface, rinse it with running water for 10 minutes, and soak the surface with 0.3% bromogeramine solution Sterilize for 20 minutes, rinse with pure water for 2 hours, and transfer to the pre-prepared ultra-clean workbench for later use. Soak the explant...

Embodiment 2

[0053] Compared with Example 1, the soaking time of the explants in the 0.1% mercuric chloride solution in step 2) was 20 minutes.

Embodiment 3

[0055] Compared with Example 1, the soaking time of the explants in the 0.1% mercuric chloride solution in step 2) was 18 minutes.

[0056] Below by using conventional 75% alcohol to soak the explants for 30 seconds to disinfect, rinse and then carry out aseptic inoculation after stem tip stripping as a control group, and adopt three embodiments of the present invention to detoxify the female plant of Lavenderia aurea The domestication situation is compared, and each group of detoxification treatment is 100 bottles, and the detoxification seedlings of the control group and the three embodiments are respectively transplanted to the greenhouse for domestication, and a comparative experiment is done under the same conditions, and the results obtained are as follows:

[0057] Group Item shoot tip survival rate Reproductive coefficient detoxification rate domestication survival rate Control group 1 0 0 0 Embodiment one 78 83.5 98.3 Embodiment two...

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Abstract

The invention discloses a method for culturing female virus-free seedlings of elaeagnus angustifolia. The method includes the steps of selection of female plants of elaeagnus angustifolia, surface disinfection of stem tips of the female plants of elaeagnus angustifolia, peeling of the stem tips of the female plants of elaeagnus angustifolia, virus detection and establishing of virus-free strains,rapid propagation of the virus-free seedlings of the female plants of elaeagnus angustifolia and domestication and culture of the virus-free seedlings of the female plants of elaeagnus angustifolia. In the method, the female plants of elaeagnus angustifolia are selected as the collecting object, and all the female plants of elaeagnus angustifolia are obtained by domestication and bloom and bear fruit after planting; the meristem of the growing point of the stem tips of the plants is used, cells in the meristem contain less or no virus, virus detection is qualified, the seedlings produced are virus free, original characters of the plants can be restored, the tuber quality and yield of elaeagnus angustifolia can be remarkably improved, and the planting risk caused by diseases and insect pests is reduced; a complete aseptic propagation system is established through the propagation and culture method by the aid of the totipotency of the plant cells, and the yield and quality of the seedlings are greatly improved.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, and in particular to a method for cultivating virus-free seedlings of Larva oleracea female plants. Background technique [0002] Jinguolan is an evergreen twining vine belonging to the genus Tinobilis. It is mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other places. Tonsillitis, acute gastroenteritis, carbuncle, diarrhea, dysentery, abdominal pain, etc. It can be made into ointment for external use, rubbed with juice or ground into powder to treat diseases such as phlebitis and venomous snake bites. The root tuber contains palmatine, columbin, isocolumbin, jatrrhizine, and tinoside. Roots contain palmitine, jatrorrhizine, coumbamine, stepharanine, dehydrodiscretamine, menisperine, magnolialine (magnoflorine), 2-deoxychitosterone (2-deoxycrustecdysone), 2-deoxy-3-epicrustecdysone (2-deoxy-3-epicrustecdysone), 2-deoxychitosterone-3-0- β-glucopyranoside (2-deoxycrust...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩明清吴昊吴顺伟尚长青
Owner 湖北金水源农业开发有限公司
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