Collagen solid beverage for improving 'screen skin' and preparation method thereof
A collagen and solid beverage technology, applied in protein-containing food ingredients, fish protein components, protein food processing and other directions, can solve problems such as poor stability of lutein, improve product mixing efficiency, increase mixing time, reduce The effect of production energy consumption
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Embodiment 1
[0034] Improve skin moisture efficacy experiment
[0035] 1.1 Experimental equipment
[0036] Experimental equipment: SHP88 skin analyzer: Courage+Khazaka, Germany;
[0037] Experimental sample: Example 7 sample, patent CN 201811137417.7 Example 5, BY-HEALTH collagen powder (peach flavor).
[0038] 1.2 Subjects
[0039] According to the relevant provisions of the 2003 edition of "Technical Specifications for Testing and Evaluation of Health Food", female subjects were selected on a voluntary basis, aged 30 to 50 years, with skin moisture ≤12. Subject exclusion criteria: ①pregnant or breastfeeding women, those with allergic constitution; ②patients with cardiovascular, cerebrovascular, liver, kidney, hematopoietic system diseases and mental illness; The judge of the result; ④ The person who did not take the sample according to the test requirements or left in the middle, could not judge the efficacy or the incomplete data would affect the efficacy or safety judgment.
[0040...
Embodiment 2
[0065] Anti-acne efficacy experiment
[0066] 1.1 Main reagents and instruments
[0067] 1.1.1 Test substance
[0068] Prepare samples according to Example 8 as the test group, the blank group is pure water, and the matched group is Kang Enbei brand Zhenmei soft capsule
[0069] 1.2 Subjects
[0070] 40 subjects aged 14-60 with facial acne and no skin lesions were selected on a voluntary basis, including 20 males and 20 females. Exclude those with heart, cerebrovascular, liver, kidney and other diseases, those who affect the judgment of the results, pregnant or lactating women, and patients with allergies.
[0071] 1.3 Experimental grouping
[0072] Before the food trial, the subjects were inspected for their vision and safety. According to gender, age and other conditions, they were randomly and double-blindly divided into a trial group, a control group and a blank group. There were 20 people in each group, 10 males and 10 females in each group. , for 20 consecutive days...
Embodiment 3
[0094] Whitening efficacy experiment
[0095] 1.1 Main reagents and instruments
[0096] Xiong Guoxi, Sigma Company; mouse melanoma cells (B16), Shanghai Cell Bank, Chinese Academy of Sciences.
[0097] RPMI-1640 medium, fetal bovine serum (FBS), 100x penicillin-streptomycin solution, mass fraction 0.25% trypsin-EDTA solution, Thermo Company, USA. CO 2 Incubator, Memmert Company, Germany; microplate reader, BioTek Company; cell counter, Biorad Company; ultrapure water machine, Thermo Company, USA; BSA224S electronic balance, Sartorius Scientific Instrument Co., Ltd., Germany; BPC-150E biochemical incubator , Electric constant temperature water bath, Shanghai Yiheng Scientific Instrument Co., Ltd.; UV-1750 ultraviolet-visible spectrophotometer, Shimadzu Corporation, Japan; desktop centrifuge, Shanghai Anting Instrument Company; high-pressure steam sterilizer, Shanghai Boxun Industry Ltd. Medical Equipment Factory.
[0098] 1.2 Test article
[0099] Arbutin was used as the ...
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