Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of 280 nm LED UV in malignant tumor autotransplantation purging in vitro under low oxygen condition

A malignant tumor and autologous technology, which is applied to special-purpose bioreactors/fermenters, biochemical instruments, biochemical cleaning devices, etc., can solve problems such as slow degradation and increased expression of target genes, and achieve the effect of reducing the recurrence rate

Pending Publication Date: 2019-08-27
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of tumor cells coping with hypoxia, the activation of hypoxia signal transduction pathway mediated by hypoxia inducible factor (HIF-1) plays an important role. Rapidly destroyed by proteasomal degradation under conditions, but slowly under hypoxic conditions, eventually leading to increased expression of target genes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of 280 nm LED UV in malignant tumor autotransplantation purging in vitro under low oxygen condition
  • Application of 280 nm LED UV in malignant tumor autotransplantation purging in vitro under low oxygen condition
  • Application of 280 nm LED UV in malignant tumor autotransplantation purging in vitro under low oxygen condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] 1. Experimental grouping

[0041] In this study, the standard cell line of acute promyelocytic leukemia cells (HL-60 cells) was used. The experiment was divided into normal control group (control group), hypoxic treatment group (hypoxic group), ultraviolet irradiation group (ultraviolet group), and hypoxic + ultraviolet co-treatment group (hypoxic + ultraviolet group). HL-60 cells were revived and subcultured according to conventional methods, and used for experiments after the cells entered the logarithmic growth phase. The cells in good growth state were taken, and the control group was cultured routinely, and the hypoxic group was given CoCl 2 (final concentration 150μmol / L) treatment, ultraviolet group with 30J / m 2 Energy 280nm wavelength ultraviolet light-emitting diode irradiation, hypoxia + ultraviolet group in the given CoCl 2 Use 30J / m on the basis of treatment 2 The energy of the 280nm wavelength ultraviolet light-emitting diodes is irradiated. Cells in e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

On a theoretical basis that ultraviolet light (UV) and cobalt chloride simulate low oxygen to induce cell apoptosis, the research uses CoCl2 to simulate a low oxygen environment and uses 280 nm LED UVto irradiate an acute promyelocytic leukemia cell (HL-60 cell), probes into proliferation situation of the HL-60 cell after the HL-60 cell is irradiated by the 280 nm LED UV under the low oxygen environment, discovers that both the 280 nm LED UV and low oxygen inhibit proliferation of the HL-60 cell and induce the cell apoptosis, and the 280 nm LED UV has more obvious proliferation inhibition andapoptosis effects on the cell under the low oxygen condition compared with a normoxic condition. Through irradiating a transplant by the 280 nm LED UV under the low oxygen condition, remaining tumorcells in the transplant are led to apoptosis and necrosis, normal cells can survive normally, so that the ultimate purpose of eliminating remaining tumors is achieved, recurrence rate of leukemia patients after autotransplantation is reduced, and application prospect of the 280 nm LED UV in leukemia autotransplantation purging in vitro is indicated.

Description

[0001] Technical field: [0002] The invention relates to a light emitting diode ultraviolet lamp (Light Emitting Diode Ultraviolet, LEDUV) and cobalt chloride chemical reagent, and the invention discloses its function in the treatment of leukemia autologous hematopoietic stem cell transplantation. [0003] Background technique: [0004] Leukemia (AL) is the most common hematological malignancy in children, mainly due to the blockage of hematopoietic stem / progenitor cell differentiation, and the result of malignant proliferation and apoptosis incompetence. Therefore, the induction of leukemia cell differentiation and (or) apoptosis is always It is considered an important strategy in the treatment of leukemia. According to FAB classification, AL can be divided into acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). At present, chemotherapy-based comprehensive treatment is used, and the curative effect has been significantly improved. The 5-year overall surviva...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/00
CPCC12M35/04C12M35/08
Inventor 孙立荣王玲珍王艺霖
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com