Hybrid peptide with functions of regulating immunity, neutralizing and digesting endotoxin and resisting inflammation, and preparation method and application thereof

Abstract

The invention relates to the technical field of gene engineering and biological agents, in particular to a hybrid peptide with functions of regulating immunity, neutralizing and digesting endotoxin and resisting inflammation, and a preparation method and application thereof. The hybrid peptide is obtained from antibacterial peptide LL-37 and thymosin T alpha 1 through designing a protein engineering computer, optimizing heterozygosity and re-screening in vivo and in vitro (abbreviated as LTA), wherein the amino acid sequence is shown as SEQ ID NO. 1. The LTA has higher bidirectional immunoregulation activity, can enhance the immunologic function of an organism under the normal or immunosuppression state and protect the damage to the organism caused by immunosuppression; in an inflammatorystate, the LTA can also neutralize endotoxin, inhibit the inflammatory reaction of organisms, relieve the damage of the inflammatory reaction to tissues, has the advantages of low cytotoxicity, high safety, convenient preparation, low cost and the like, can be used as an ideal immune anti-inflammatory regulator, and has good application potential and value.

Description

technical field [0001] The invention relates to the field of genetic engineering and biological preparations, in particular to a hybrid peptide with functions of immune regulation, neutralization and digestion of endotoxin and anti-inflammation, its preparation method and application. Background technique [0002] When animals or humans are young, weak, disease, stress, pathogenic infection, etc., their immunity is often reduced, and then secondary infection (bacterial or viral mixed infection) occurs, causing inflammatory reactions (red, hot, swollen) , pain, etc.). At present, for the treatment of infection and inflammation, the traditionally commonly used prevention and treatment strategy is to use antibiotics and steroid anti-inflammatory drugs (such as hydrocortisone, dexamethasone, etc.), although it can effectively control infectious inflammation and non-infectious inflammation, However, continuous use can cause various side effects: such as serious disturbance of wa...

AI Technical Summary

Problems solved by technology

There are also obvious problems in the use of antibiotics or hormonal anti-inflammatory drugs in the treatment of infection and inflammation: although antibiotics can reduce or kill pathogenic bacteria, they cannot improve the immune function of the body. On the contrary, the bacteria killed by antibiotics also produce endotoxin or Exotoxins, which in turn aggravate the inflammatory response and even lead to systemic inflammatory response syndrome (Botwinski, 2001), which can cause fever, anorexia, excessive energy consumption in the body, decomposition of body tissues, and decline in immunity and production performance in animals. Avian mortality (Botwinski, 2001; Zinner, 1999)

Therefore, in the field of traditional medicine, the clear separation of anti-inflammatory drugs and immune-enhancing drugs does not meet the actual needs of the body or the principle of the interaction between anti-inflammatory and immune, and greatly increases the complexity and antagonism of clinical drug selection.

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