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Seneca valley virus, preparation method of seneca valley virus inactivated vaccine, seneca valley virus inactivated vaccine and application thereof

A virus inactivation and virus technology, applied in the field of veterinary biological products, can solve the problems of high lethality of newborn piglets, difficulty in identification and differentiation, panic of farmers, etc., and achieve good protection effect, broad application prospect and good safety effect.

Active Publication Date: 2019-04-26
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0003] Porcine Senega Valley virus, as an exotic pathogen, has been scattered in China, and may show a trend of spreading in the next few years. Although swine Senega Valley virus disease will not cause the same major economic losses as foot-and-mouth disease virus, the disease has a significant impact on Newborn piglets also have a high mortality rate, and their clinical symptoms are highly similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. economic loss
Therefore, in view of the fact that there is no commercialized safe and efficient porcine senega valley virus disease inactivated vaccine at present in my country, it is extremely urgent to develop an inactivated porcine senega valley virus disease vaccine. For preventing porcine senega valley virus disease The invasion has important strategic reserve significance

Method used

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  • Seneca valley virus, preparation method of seneca valley virus inactivated vaccine, seneca valley virus inactivated vaccine and application thereof
  • Seneca valley virus, preparation method of seneca valley virus inactivated vaccine, seneca valley virus inactivated vaccine and application thereof
  • Seneca valley virus, preparation method of seneca valley virus inactivated vaccine, seneca valley virus inactivated vaccine and application thereof

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preparation example Construction

[0029] The present invention also provides a kind of preparation method of porcine Senega Valley virus inactivated vaccine, comprises the following steps:

[0030] 1) Cultivating PK-15 cells with cell culture medium, when the PK-15 cells grow into a single layer, discarding the cell culture medium, replacing it with DMEM nutrient solution, and culturing for 48-60 hours to obtain a culture;

[0031] The DMEM nutrient solution contains the porcine senegal virus according to claim 1, and the volume concentration of the porcine senegal virus is 0.1 to 0.2%;

[0032] 2) Filtrating the culture obtained in step 1), adjusting the pH of the obtained filtrate to 7.6 to 7.8, and then mixing it with divethyleneimine, and inactivating the obtained mixture to obtain an inactivated virus liquid;

[0033] The concentration of divinylimine in the mixture is 1.5 to 2.5 mmol / L;

[0034] 3) Mix and emulsify the inactivated virus liquid obtained in the step 2) with an adjuvant to obtain an inacti...

Embodiment 1

[0055] 1. Poison species

[0056] 1.1 Source of the virus: The virus used for the production and inspection of the inactivated vaccine of swine senega virus disease (A / ZJ / 2015) is the strain of porcine senega virus A / ZJ / 2015, which is stored in China Microorganisms General Microbiology Center of the Preservation Management Committee, the deposit number is CGMCC No.15034.

[0057] 1.2 PCR detection: RNA was extracted from 10 skins of pig feet clinically diagnosed as suspected SVV, treated with penicillin and streptomycin, and reacted with RT-PCR. The PCR products were identified by gel electrophoresis, and the results showed that 5 samples were pig plugs Negagu virus positive. 5 positive samples were inoculated into PK-15 cells, and RT-PCR was performed after 3 generations of blind passage. Among them, 4 samples were positive for SVV. The electrophoresis results are shown in figure 1 . The strain isolated from sample No. 2 was named: A / ZJ / 2015; the strain isolated from sampl...

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Abstract

The invention provides a seneca valley virus A / ZJ / 2015, a preparation method of a seneca valley virus inactivated vaccine, the seneca valley virus inactivated vaccine and an application thereof, and belongs to the technical field of veterinary biological products. The Latin name of the seneca valley virus is seneca valley, and the preservation number is CGMCC No.15034. According to the invention,the seneca valley virus is cultured to prepare the inactivated vaccine for preventing seneca valley virus diseases. The vaccine has good immunogenicity to healthy susceptible one month old pigs, and the protection rate after inoculation is 90% or above.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a preparation method of porcine senega valley virus A / ZJ / 2015, an inactivated porcine senega valley virus vaccine, and an application thereof. Background technique [0002] Swine Seneca Valley virus disease is a pig primary herpes disease caused by Seneca Valley virus (SVV). It is very similar to foot-and-mouth disease (FMD), swine vesicular disease (SVD), swine vesicular eruption (VES) and vesicular stomatitis (VS), and it is difficult to distinguish it clinically. In 2008 and 2012, SVV was diagnosed in diseased pig herds in parts of the United States and Canada. In September 2015, vesicular outbreaks occurred in pig herds in several regions in the Midwest of the United States. Blister-like lesions appeared on the snout and hooves of infected pigs. Diarrhea symptoms were occasionally seen, and the disease was rapid. The mortality rate of newborn piglets w...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/125A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552A61P31/14C12N7/00C12N2770/32021C12N2770/32034
Inventor 吕建亮方鹏飞潘丽李妍马中元吴学婧张中旺李捷刘新生严欢周鹏王永录张永光
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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