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Preparation method and application of artificial cartilage framework

A cartilage and artificial technology, which is applied in the field of preparation of artificial cartilage scaffolds, can solve the problems that cryogenic technology cannot completely remove cell debris, decellularization technology is not thorough enough to remove, and difficult to heterogeneous immunogenicity, so as to avoid local collapse and shorten Effects of surgery time, good shape and support

Inactive Publication Date: 2019-04-09
SUMMIT GD BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the heterogeneous cartilage raw material is very dense and there are many chondrocytes, it is difficult to completely remove its heterogeneous immunogenicity
There are deficiencies in the existing treatment technologies, such as the simple decellularization technology is not thorough enough, and the cryogenic technology cannot completely remove the cell debris

Method used

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  • Preparation method and application of artificial cartilage framework
  • Preparation method and application of artificial cartilage framework
  • Preparation method and application of artificial cartilage framework

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preparation example Construction

[0031] In some embodiments, the preparation method of the artificial cartilage scaffold of the present invention includes steps such as pretreatment, drilling, degreasing, decellularization, antigen removal, cross-linking and fixation, and specifically includes the following steps:

[0032] a. Pretreatment: take fresh animal cartilage tissue, wash, disinfect, and remove fat tissue, fiber and fluff tissue;

[0033] b. Drilling: using mechanical or laser drilling, the hole diameter is 30-100 microns, the hole spacing is 0.5-2.0 mm, and the hole depth is 50-200 microns;

[0034] c. Degreasing: Animal tissue is extracted by supercritical carbon dioxide or organic solvent extraction to remove fat and fat-soluble impurities. The degreasing rate reaches more than 99%, and the residual fat mass ratio is 0-0.5%;

[0035] d. Decellularization: Use a surfactant solution (one or more of small molecule organic acid anhydrides, acid chlorides, amides, epoxides, and methyl halides) combined ...

Embodiment 1

[0040] A kind of embodiment of the preparation method of artificial cartilage support of the present invention, comprises the steps:

[0041]a. Pretreatment: take fresh animal cartilage tissue, wash, disinfect, and remove fat tissue, fiber and fluff tissue;

[0042] b. Drilling: using mechanical drilling, the hole diameter is 30 microns, the hole spacing is 0.5 mm, and the hole depth is 50-200 microns;

[0043] c. Degreasing: animal tissue adopts supercritical carbon dioxide extraction method to remove fat and fat-soluble impurities, the degreasing rate reaches 97%, and the residual fat mass ratio is 0.15%;

[0044] d. Decellularization: use surfactant-organic acid anhydride solution combined with protease solution, control the mass final concentration of surfactant to 0.2%, and the mass final concentration of protease to 0.25%, and carry out enzymolysis of cells in the cartilage tissue under ultrasonic conditions, Use sterile ultrapure water to clean the animal cartilage und...

Embodiment 2

[0048] A kind of embodiment of the preparation method of artificial cartilage support of the present invention, comprises the steps:

[0049] a. Pretreatment: take fresh animal cartilage tissue, wash, disinfect, and remove fat tissue, fiber and fluff tissue;

[0050] b. Drilling: using laser drilling, the hole diameter is 66 microns, the hole spacing is 1.2 mm, and the hole depth is 50-200 microns;

[0051] c. Degreasing: The animal tissue adopts organic solvent extraction method to remove fat and fat-soluble impurities. The degreasing rate reaches 99%, and the mass ratio of residual fat is 0.05%;

[0052] d. Decellularization: use a surfactant solution (a mixed solution of acid chloride and amide, wherein the mass ratio of acid chloride and amide is 1:3) and in combination with the protease solution, the mass final concentration of the control surfactant is 0.3%, the mass of the protease The final concentration is 0.2%, and the cells in the cartilage tissue are enzymolyzed u...

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Abstract

The invention discloses a preparation method of an artificial cartilage framework. The preparation method of the artificial cartilage framework comprises the steps of preprocessing, punching, degreasing, decellularizing, removing an antigen, fixing in a crosslinking way and the like. The artificial cartilage framework has a natural radian, when the artificial cartilage framework is applied to alanasi, apex nasi, a nose bridge bone and the like, the trimming is reduced, the operative process is simplified, and the operation time is shortened; the toughness and the mechanical strength of the artificial cartilage framework are special, the elasticity is relatively good, the local collapse, deformation and the like caused after the implantation is avoided, and the shaping and supporting effects are excellent; the artificial cartilage framework is hydrolyzed by protease in the body after being implanted and reconstructed with the autologous tissue; the artificial cartilage framework controls the biodegradability of the framework through the crosslinking fixing process, individualized framework products having different degradation rates are customized according to the different operation requirements, and no residual toxicily is caused after the degradation.

Description

technical field [0001] The invention relates to the technical field of orthopedic materials, in particular to a preparation method and application of an artificial cartilage scaffold. Background technique [0002] At present, the materials used for rhinoplasty repair, ear plastic repair, jaw plastic repair and other operations mainly come from synthetic materials, autologous cartilage, artificial cartilage repair materials, etc.; one of the synthetic materials mainly includes silica gel and expanded polytetrafluoroethylene. etc. After implantation, silicone materials have disadvantages such as aging, denaturation, exposure, and easy displacement, while expanded polytetrafluoroethylene (ePTFE) is not resistant to infection, and once infected, it must be completely removed, causing great harm to beauty lovers The second is autologous cartilage, such as costal cartilage and ear cartilage taken from the beauty lover. This type of material has no foreign body rejection, but its s...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36A61L27/56
CPCA61L27/3612A61L27/3654A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2430/06A61L2430/40
Inventor 张伟胡康唐容
Owner SUMMIT GD BIOTECH
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