Preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release

A nanomaterial, photothermal therapy technology, applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, nanotechnology, etc. Reduce toxic and side effects, achieve drug release, and achieve the effect of tumor tissue-specific accumulation

Active Publication Date: 2022-03-18
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional anti-tumor chemotherapeutic drugs often lack selectivity and are widely distributed in the body after administration. The therapeutic dose has severe side effects on normal tissues and organs, causing great pain to patients and poor curative effect.

Method used

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  • Preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release
  • Preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release
  • Preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of multi-action nanomaterials with targeted photothermal therapy and controlled drug release

[0045] A method for preparing a multi-action nanomaterial with targeted photothermal therapy and controlled drug release, the steps are as follows:

[0046] (1) Preparation of polydopamine nanomaterials

[0047] Take 1.5mL of ammonia water (mass concentration 25-28%), 45mL of absolute ethanol, and 80mL of deionized water in a round bottom flask, and stir at 35°C for 20 minutes to obtain ammonia water / ethanol / deionized aqueous solution; weigh dopamine hydrochloride (purchased Dissolve 30 mg from Sigma-Aldrich, H8502-5G) in 10 mL of deionized water to obtain an aqueous solution of dopamine hydrochloride; add the aqueous solution of dopamine hydrochloride to the above-mentioned ammonia / ethanol / deionized aqueous solution, and react with stirring at 35°C and 150 rpm for 30 hours After the reaction, centrifuge at 8000rpm for 30 minutes to collect the precipita...

Embodiment 2

[0061] The light-to-heat conversion performance of the polydopamine nanomaterial that the glucose link of embodiment 2

[0062] (1) Configuration of the reaction system

[0063] Weigh 10 mg of the glucose-linked polydopamine nanomaterial prepared in step (2) of Example 1, disperse in 10 mL of phosphate (PBS) buffer, and serially dilute to obtain 10 μg / mL, 20 μg / mL, 50 μg / mL, 100 μg / mL and 200μg / mL suspension. In the experimental group, 200 μL of glucose-linked polydopamine nanomaterial suspension was added to a single well of a 96-well plate; in the control group, 200 μL of PBS buffer was added to a single well of a 96-well plate;

[0064] (2) Test of light-to-heat conversion performance

[0065] The near-infrared light emitted by the BST808-5-F laser in the experimental group and the control group was 3.6W / cm 2 Irradiate for 5 minutes under the same conditions, and use an electronic thermometer to measure and record the temperature change every 30 seconds. The results are ...

Embodiment 3

[0066] Example 3 Drug release of multi-action nanomaterials with targeted photothermal therapy and controlled drug release under different pH conditions

[0067] Weighed 20 mg of the multiple-action nanomaterial with targeted photothermal therapy and controlled drug release prepared in Example 1, resuspended with 4 mL of PBS buffer (10 mM, pH 7.4, containing 0.1% Tween20), and set up 3 repeated experiments, For each experiment, 1 mL of multi-action nanomaterial suspension with targeted photothermal therapy and controlled drug release was placed in a dialysis bag (MW: 7000), and the dialysis bag was dialyzed in 10 mL of a 15 mL centrifuge tube and 10 mM dialysate. Set 4 groups of different conditions, including: 1) the dialysate is PBS buffer with pH 7.4; 2) the dialysate is PBS buffer with pH 6.5; 3) the dialysate is PBS buffer with pH 5.0; 4) the dialysate is pH 5.0 PBS buffer solution and near-infrared light (2W / cm2) emitted by BST808-5-F laser 2 ) irradiated for 3 minutes....

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Abstract

The invention relates to a preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release. The multi-action nanomaterial with targeted photothermal therapy and controlled drug release in the present invention uses glucose as a targeting molecule, which is covalently linked to polydopamine nanomaterials with photothermal conversion capabilities, and through Polydopamine nanomaterials loaded with antitumor drugs. The multi-function nanomaterial prepared by the present invention has multiple functions of targeting, photothermal and pH-responsive drug release, can realize tumor tissue-specific accumulation and drug release, reduce the toxic and side effects of chemotherapy drugs on the human body, and can target The different weaknesses of tumor tissue can achieve the effect of high-efficiency combined treatment of tumors, and the multi-action nanomaterial has high biocompatibility, non-toxicity, no pollution, simple preparation process, and low cost, and is suitable for large-scale production and clinical medical use .

Description

technical field [0001] The invention relates to a preparation method and application of a multi-action nanomaterial with targeted photothermal therapy and controlled drug release, belonging to the technical field of therapeutic drugs. Background technique [0002] Tumor is the second leading cause of death in the world. Traditional surgical treatment, radiotherapy and chemotherapy have the disadvantages of high invasiveness and high side effects, and there is an urgent need to develop new treatment methods and strategies. In tumor therapy, it is an important strategy for tumor therapy to develop therapeutic agents with multiple functions aimed at the weakness of tumor tissue, to improve anti-tumor effects and reduce toxic and side effects. The main feature of tumor cells is continuous and rapid proliferation. Tumor cells will increase glucose intake for glycolysis to provide the energy they need. Studies have shown that tumor cells usually overexpress glucose transporters (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/05A61K41/00A61K47/54A61K47/59A61P35/00B82Y5/00
CPCA61K38/05A61K41/0052A61K47/549A61K47/59A61P35/00B82Y5/00A61K2300/00
Inventor 徐莉肖敏李银萍洪伟英张甜甜
Owner SHANDONG UNIV
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