A nano drug delivery system loaded with 5-aminolevulinic acid and its preparation method and application
A technology of aminolevulinic acid and delivery system, which is applied in the field of nano drug delivery system loaded with 5-aminolevulinic acid and its preparation, which can solve the problems such as difficult to pass through the biofilm barrier, achieve convenient preparation and use, and reduce drug side effects Small, well-dispersed effect
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[0022] The preparation method of the above-mentioned nano drug delivery system is as follows:
[0023] Dissolve CDG2 in water, slowly add the aqueous solution of 5-ALA, the molar ratio of CDG2 to 5-ALA is 1:(2~3), stir for 22~28h, and ultrafilter to obtain CDG2 / 5-ALA complex ; Slowly add the aqueous solution of hyaluronic acid to the aqueous solution of the CDG2 / 5-ALA complex, stir for 11 to 13 hours, and ultrafilter to obtain the product. Specifically, its preparation method includes: weighing 110-130 mg of CDG2, dissolving in water, slowly adding 150-170 mg of 5-ALA aqueous solution, stirring for 22-28 hours, ultrafiltration, and freeze-drying to obtain the CDG2 / 5-ALA complex ; Slowly add 110-120 mg of hyaluronic acid aqueous solution to the CDG2 / 5-ALA complex aqueous solution, stir for 11-13 hours, ultra-filter and freeze-dry to obtain. In some embodiments, the ultrafiltration is: ultrafiltration at 10000-14000 rpm for 18-22 minutes. Preferably, the ultrafiltration condit...
Embodiment 1
[0039] The preparation process of the nano drug delivery system CAH of the synergistic 5-ALA treatment of melanoma in this example is as follows figure 1 As shown, the nano-drug delivery system CAH-NH 2 with -COOH(M -NH2 / M -COOH ) molar ratio is 3:1, and its preparation method comprises the following steps:
[0040] (1) Preparation method of CDG2 / 5-ALA complex
[0041] Weigh 120 mg of a lyophilized sample of CDG2 purified by dialysis and dissolve it in 5 mL of ultrapure water. After it was completely dissolved, slowly add 160 mg of 5-ALA aqueous solution, wherein the molar ratio of 5-ALA carboxyl group to CDG2 amino group was 2:1, and stirred on a magnetic stirrer for 24 hours at room temperature and protected from light. The resulting reaction solution was ultrafiltered and centrifuged for 20 min at 12000 rpm, and freeze-dried to obtain the CDG2 / 5-ALA complex. The content of free 5-ALA not bound to CDG2 / 5-ALA in the supernatant was detected by ultraviolet light.
[0042...
Embodiment 2
[0045] A nano drug delivery system (CAH) for the treatment of melanoma with synergistic 5-ALA in this embodiment, the molar ratio of CAH (M-NH2 / M-COOH) is 2:1, and its preparation method includes the following steps:
[0046] (1) Preparation method of CDG2 / 5-ALA complex
[0047] Weigh 120 mg of a lyophilized sample of CDG2 purified by dialysis and dissolve it in 5 mL of ultrapure water. After it was completely dissolved, slowly add 160 mg of 5-ALA aqueous solution, wherein the molar ratio of 5-ALA carboxyl group to CDG2 amino group was 2:1, and stirred on a magnetic stirrer for 24 hours at room temperature and protected from light. The resulting reaction solution was ultrafiltered and centrifuged for 20 min at 12000 rpm, and freeze-dried to obtain the CDG2 / 5-ALA complex. The content of free 5-ALA not bound to CDG2 / 5-ALA in the supernatant was detected by ultraviolet light.
[0048] (2) Preparation of CAH
[0049] Dissolve the CDG2 / 5-ALA complex prepared above in 5mL ultrapur...
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