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Method for producing high-quality biological feed from multiple bacteria through composite production

A compound fermentation and biological feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of uneven fermentation of biological feed, low utilization rate and conversion rate of base materials, and achieve the promotion of growth and synthesis of metabolites, Solve the problem of biological resource utilization, no environmental pollution effect

Inactive Publication Date: 2019-03-22
江苏春之雨生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of with oyster mushroom, saccharomycete and white rot fungus as composite bacterial classification, combine the method for producing high-quality biological feed with liquid fermentation and solid fermentation, solve the inhomogeneous fermentation of biological feed in the prior art, Improve the palatability and nutritional value of the feed due to the low utilization rate and conversion rate of the base material

Method used

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  • Method for producing high-quality biological feed from multiple bacteria through composite production
  • Method for producing high-quality biological feed from multiple bacteria through composite production
  • Method for producing high-quality biological feed from multiple bacteria through composite production

Examples

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Effect test

Embodiment 1

[0049] A method for producing high-quality biological feed through multi-strain compound fermentation, the method comprises the following steps:

[0050] 1. Activation and expansion of strains

[0051] 1) Pleurotus ostreatus: Select Guangwen Pleurotus ostreatus "Suping No. 1", and inoculate the strain of Pleurotus ostreatus into the improved PDA medium (the components of the medium are calculated as 1L: peeled potato 200g, glucose 20g, agar Powder 20g, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, FeSO 4 ·7H 2 O 5mg, CuSO 4 ·5H 2 O 0.3mg, vitamin B1 10mg, the balance is purified water, natural pH value) on the inclined surface, cultured at 28°C for activation, and after the mycelia covered the inclined surface, cut the mycelium block into 1cm 2 Small pieces, inoculate 1-2 pieces into 100mL liquid medium (the components of the medium are calculated as 1L: glucose 30g, yeast extract 4.1g, peptone 2.8g, wort juice 12.5ml, KH 2 PO 4 3.0g, MgSO 4 ·7H 2 O1.5g, zinc methionine 2.8mg...

Embodiment 2

[0061] Effect of Different Inoculation Ratio on Composite Liquid Fermentation

[0062] According to the method described in embodiment 1, in step 2, 10% of total inoculum size is constant, and the inoculation ratio of flat mushroom, Candida utilis and Phanerochaete chrysosporium bacterial classification is respectively set to 4:3:3,3: 3:4, 2:1:2, 1:2:2, in order to study the influence of the inoculum ratio on the compound liquid fermentation, carry out the dry weight of the bacterial cell, protease activity and cellulase activity measurement to the obtained compound fermentation liquid, the result is as follows figure 1 and figure 2 shown.

[0063] from figure 1 and figure 2 It can be seen that when the strain ratio is 4:3:3, the dry weight of the thalline, protease activity and cellulase activity all reach the maximum value, indicating that under this strain ratio, Pleurotus ostreatus, Candida utilis and Phanerochaete chrysosporium reached a good symbiotic relationship,...

Embodiment 3

[0065] Effects of Different Culture Temperatures on Composite Liquid Fermentation

[0066] According to the method described in Example 1, the culture temperature in step 2 was set to 26°C, 28°C, 30°C, and 32°C respectively to study the influence of the culture temperature on the fermentation of the compound liquid, and to dry the obtained compound fermentation liquid. Weight, protease activity and cellulase assay, the results are as follows image 3 and Figure 4 shown.

[0067] from by image 3 It can be seen that when the culture temperature is 28°C and 30°C, the dry weight of the bacteria reaches the maximum value; Figure 4 It can be seen that when the culture temperature is 28°C, the activity of protease is the highest, but it is not significantly different from that at 30°C; when the culture temperature is 30°C, the activity of cellulase is the highest, but it is not significantly different from that at 28°C The difference in enzyme activity was not significant, so ...

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Abstract

The invention provides a method for producing high-quality biological feed from multiple bacteria through composite production. The method comprises the following steps: 1, carrying out bacterium activation and culture amplification; 2, carrying out composite liquid fermentation; 3, carrying out dual radiation treatment on a fermentation liquid; 4, carrying out solid fermentation. By adopting themethod, oyster mushroom, saccharomycetes and white-rot fungi are subjected to composite fermentation, and by virtue of synthetic functions of different bacteria, complex metabolic activity which is hard to complete by single bacteria can be cooperatively completed. In the culture process, the bacteria are in mutualism, the ratio of an enzyme system is harmonized, the enzyme yield of composite fermentation is greatly greater than that of a single bacterium, then the fermentation efficiency and the bioavailability of the biological feed can be improved, and the quality of the biological feed isgreatly improved. By adopting the method provided by the invention, liquid fermentation and solid fermentation are combined, the biological conversion rates of straw and soybean cakes are increased, the problems that substrates of a conventional single solid fermentation method cannot be sufficiently utilized and a great amount of raw materials are wasted can be solved, the waste is turned into wealth, and the application values of the straw and the soybean cakes are further increased.

Description

technical field [0001] The invention belongs to the field of feed production, and in particular relates to a method for producing high-quality biological feed through multi-strain compound fermentation. Background technique [0002] my country is a large agricultural country, and animal husbandry has always played an important role in my country's agricultural economy. With the rapid increase of national economic strength and the continuous improvement of per capita income, the demand for animal food such as meat, eggs, milk, and aquatic products is increasing, and the demand for feed in the breeding process is also expanding. Although my country's annual feed output is currently the world's largest, the feed industry is still in short supply of resources, and some high-quality feed resources mainly rely on imports. For example, my country's imports of protein feed soybeans and fishmeal rank first in the world, and the import dependence is as high as 70%, which also greatly...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K10/30
CPCA23K10/12A23K10/30A23K10/37Y02P60/87
Inventor 孙倩陈甲子
Owner 江苏春之雨生物科技发展有限公司
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