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Application of MPLA in the preparation of drugs for prevention and treatment of testicular injury induced by ionizing radiation

An ionizing radiation, testicular technology, applied in the field of medicine, can solve problems such as testicular damage, achieve the effects of less toxic and side effects, increase sperm motility and sperm count, and reduce the degree of necrosis

Inactive Publication Date: 2019-01-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, drugs used for intestinal radiation damage cannot simply be used to prevent and treat testicular damage caused by ionizing radiation
[0005] However, there are no relevant reports in the existing literature and patents on the application of MPLA in the preparation of drugs for the prevention and treatment of testicular damage caused by ionizing radiation.

Method used

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  • Application of MPLA in the preparation of drugs for prevention and treatment of testicular injury induced by ionizing radiation
  • Application of MPLA in the preparation of drugs for prevention and treatment of testicular injury induced by ionizing radiation
  • Application of MPLA in the preparation of drugs for prevention and treatment of testicular injury induced by ionizing radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Firstly, a mouse model of radiation-induced testicular injury was established, and 18 male C57BL / 6 mice aged 6-8 weeks were selected and randomly divided into 3 groups, 6 mice in each group: 3 mice in the irradiation group (4Gy) and 12 hours before irradiation with MPLA. 3 rats in the drug group (4Gy+MPLA) and 3 rats in the control group; 60 Coγ-rays were irradiated to the abdomen of the mice once, and the absorbed dose of the mice was 4Gy. MPLA (administration dose 50 μg / kg, MPLA dissolved in 0.1 ml physiological saline) or physiological saline (0.1 ml / mouse) were delivered to the corresponding groups by intragastric administration. On the first day and the seventh day after radiation exposure, the testes of 8 mice in each group were taken respectively, fixed, embedded in wax blocks, and sectioned for HE staining (such as figure 1 shown). Observation under the microscope showed that the structure of the testis in the non-irradiated group was complete, the cells were ...

Embodiment 2

[0038] (1) The radiation-induced testicular injury mouse model is the same as that in Example 1;

[0039] (2) Male TLR4 knockout mice aged 6-8 weeks were selected and randomly divided into 3 groups, 3 mice in each group: the simple irradiation group and the control group were intragastrically administered with normal saline (0.1ml / mouse) 12 hours before irradiation, The irradiation administration group was administrated with MPLA 12 hours before irradiation (administration dose was 50 μg / kg, MPLA was dissolved in 0.1ml normal saline), and the mice were killed on the seventh day after irradiation, and the testis tissues were taken, fixed, embedded in wax blocks, and sectioned. followed by HE staining (e.g. figure 2 Shown), under the microscope, it was found that the structure of the testis in the non-irradiated group was complete, the cells were large, the seminiferous tubules were full, and the outline was clear, which was not significantly different from that of the wild-typ...

Embodiment 3

[0041] (1) The radiation-induced testicular injury mouse model is the same as that in Example 1;

[0042] (2) Select 6 male C57BL / 6 mice aged 6-8 weeks, and randomly divide them into 2 groups, 3 mice in each group. The administration group was administrated with MPLA 12 hours before irradiation (administration dose was 50 μg / kg, MPLA was dissolved in 0.1ml normal saline), and the mice were killed 16 hours after irradiation, and the testis tissues were taken, fixed, embedded in wax blocks, sliced, Carry out TUNEL (terminal dexynucleotidyl transferase (TdT)-mediateddUTP nick end labeling, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay) staining, observe under a microscope, and randomly select 10 low-magnification fields of view for TUNEL-positive cells Count and calculate the average (such as image 3 As shown), the results showed that the testicular TUNEL positive cells in the simple irradiation group were significantly more than those in the irrad...

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Abstract

The invention relates to the technical field of medicine, in particular to the application of glucopyranoside lipid A (MPLA) in the preparation of medicine for preventing and treating testicular damage caused by ionizing radiation. The MPLA provided by the invention has the advantages of small toxicity and side effect, remarkable curative effect of radiation injury prevention and treatment of testis, safety and convenience of medication and the like as a medicine for preventing and treating testicular injury caused by ionizing radiation. These properties show the unique advantages of MPLA in the prevention and treatment of radiation-induced testicular injury, aiming at exploring new and more effective methods and techniques for the prevention and treatment of radiation-induced testicular injury. However, there is still a lack of effective prevention and treatment of radiation damage to male reproductive system at home and abroad, and solving this problem has become a key demand in thefield of radiation medicine. Therefore, MPLA has a broad application prospect in the field of medicine in China as the preparation of ionizing radiation-induced testicular injury prevention and treatment drugs.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of MPLA (glucopyranoside lipid A) in the preparation of drugs for the prevention and treatment of testicular damage caused by ionizing radiation. Background technique [0002] With the wide application of nuclear science and technology, wartime nuclear radiation, nuclear terrorist attacks, nuclear reactor accidents in normal times, and clinical radiotherapy for malignant tumors can all cause serious damage to the testes by ionizing radiation, resulting in serious consequences such as infertility, carcinogenesis, and teratogenicity. . Testicular radiation protection is also the focus of radiation protection for male doctors of reproductive age in the departments of nuclear medicine, interventional therapy, radiotherapy and imaging. The spermatocytes and spermatogonia in the testis are extremely sensitive to ionizing radiation. After the testis is damaged by ioniz...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61P15/00A61P15/08
CPCA61K31/7024A61P15/00A61P15/08
Inventor 刘哲高福杨彦勇蔡建明曹堃陈媛媛雷霄曲红金刘蕾廖泽彬
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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