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Carbon dot-molecularly imprinted test strip for detecting tyramine as well as preparation method and application

A molecular imprinting and test strip technology, which is applied in the field of preparation and detection of carbon dot-molecularly imprinted polymer test strips, can solve the problems of less research on fluorescence-molecular imprinting methods and the like

Active Publication Date: 2019-01-18
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the development of immunoassay test strips is relatively mature, but there are few studies on fluorescent-molecular imprinting methods based on test strips

Method used

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  • Carbon dot-molecularly imprinted test strip for detecting tyramine as well as preparation method and application
  • Carbon dot-molecularly imprinted test strip for detecting tyramine as well as preparation method and application
  • Carbon dot-molecularly imprinted test strip for detecting tyramine as well as preparation method and application

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Embodiment 1

[0036] The invention provides a preparation method and application of a carbon dot-molecularly imprinted polymer test strip for detecting tyramine, and the specific preparation route is as follows:

[0037] (1) Dry Whatman filter paper in a vacuum drying oven for 30 minutes to remove moisture, cut it into a rectangular paper strip with a size of 0.9×2cm, and soak it in γ-methacryloxy with a concentration of 80% (v / v) Propyltrimethoxysilane (KH-570) ethanol solution, incubate at room temperature for 1 hour, rinse with absolute ethanol three times, then air dry for later use;

[0038] (2) Preparation of carbon dots: Dissolve 0.8 g of anhydrous citric acid (carbon source) in 16.0 mL of deionized water, degas with nitrogen for 15 min, inject 4.0 mL of APTES (functionalization reagent) into it and stir. The obtained precursor solution was transferred into a 50 mL autoclave, and subjected to hydrothermal treatment at 200 °C for 2 h. After cooling to room temperature, use a 1kDa dia...

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Abstract

The invention discloses a preparation method and application of a carbon dot-molecularly imprinted test strip for detecting tyramine. The preparation method comprises the following steps: introducingdouble bonds to the surface of Whatman filter paper as a solid carrier with a silane reagent; then dissolving carbon dots, tyramine, 3-aminopropyl triethoxysilane, tetraethoxysilane and ammonia waterin anhydrous ethanol; adding a pre-treated carrier; and grafting a carbon dot-molecularly imprinted polymer on the surface of the filter paper. The molecularly imprinted polymer contains tyrosine recognition and binding sites, so that specific binding to target molecules can be realized. The fluorescence quenching can be achieved through electron transfer between tyrosine and carbon dots, thus achieving the detection purpose. The detection method has the advantages of simple and fast operation and low preparation cost, and can be applied to the detection of tyramine in foods.

Description

technical field [0001] The invention belongs to the technical field of optical sensing materials and molecular imprinting, and in particular relates to the preparation and detection method of a carbon dot-molecular imprinting polymer test strip for detecting tyramine. Background technique [0002] Tyramine (TA), also known as p-hydroxyphenylethylamine, is a kind of biogenic amine containing nitrogen and low molecular weight. It has important physiological effects on animals, plants and microorganisms. It mainly exists in mature cheese, aged cheese, fermented meat, In foods such as soy sauce. The compound has pharmacological effects such as raising blood pressure and stimulating the uterus. High concentrations will not only seriously affect the flavor of food and even change its composition, but also have a serious toxic effect on the human body. It can promote peripheral vasoconstriction, stimulate heart rate, enhance respiration, increase blood sugar concentration, and eli...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/94G01N33/58G01N33/533
CPCG01N21/6428G01N33/533G01N33/582G01N33/9406G01N2021/6432
Inventor 王硕乔丹邓启良
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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