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High-throughput detection device and method for rapid analysis of Raman spectrum

A rapid analysis and Raman spectroscopy technology, applied in measurement devices, material analysis by optical means, Raman scattering, etc., can solve the problems of time-consuming and laborious preparation, reducing the utilization rate of optical discs, and large losses, and achieves reduction of operation steps, High-throughput Raman signal acquisition, the effect of promoting field detection applications

Inactive Publication Date: 2020-01-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is simple and effective in structure, but it is usually produced by compression molding and injection molding, which is time-consuming and laborious to prepare.
In addition, the centrifuge channel reduces the usage rate of the disc, and the sample liquid with higher viscosity loses more through centrifugation

Method used

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  • High-throughput detection device and method for rapid analysis of Raman spectrum
  • High-throughput detection device and method for rapid analysis of Raman spectrum
  • High-throughput detection device and method for rapid analysis of Raman spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] In this example, 40nm gold nano-ions are used as the enhanced substrate, and the special nitrogen-containing structure in malachite green—dimethylamino can be well adsorbed on the surface of the gold substrate. By combining the high-throughput rotating detection disc, high-pass Amount of malachite green detected.

[0045] 1) Preparation and concentration of gold nanoprobes

[0046] Colloidal gold particles of 40 nm were prepared by trisodium citrate reduction method. Before the experiment, first soak the glassware (round bottom flask, condenser tube and beaker) in aqua regia for 24 hours, then rinse repeatedly with distilled water, and then soak in ultrapure water overnight; take out the vessel and dry it for use; accurately measure 99mL of ultrapure water Pure water was placed in a round bottom flask, and a magnetic stirring rotor was added at the same time; 1 mL of chloroauric acid (mass fraction 1%) was added, and the heating and stirring switch in the heating mantl...

Embodiment 2

[0053] In this embodiment, 40nm gold nano-ions are used as gold nano-seeds, and silver nanoparticles are further wrapped on this basis to improve the detection signal of SERS. By combining high-throughput rotating detection discs, high-throughput rhodamine B detection is realized. .

[0054] 1) Preparation of silver-coated gold nanoprobes

[0055] On the basis of the 40nm nano colloidal gold particles synthesized in Example 1, further prepare silver-coated gold nanoparticles, draw 10 mL of gold nano seed solution in a round bottom flask, and add 1.2 mL of 0.1 mol L successively while stirring. -1 Ascorbic acid, stir evenly at room temperature for 5 minutes, and finally slowly add 1 mL of 2.5mM silver nitrate dropwise, continue stirring at room temperature in the dark for 30 minutes, and store in a 4°C refrigerator for later use.

[0056] 2) Mixture of Raman-enhanced substrate and rhodamine B

[0057] Take an equal volume of AuNPs particles and 0.01 μg·mL -1 After mixing rho...

Embodiment 3

[0061] In this embodiment, 40nm gold nano-ions are used as the enhanced substrate, and crystal violet can be well adsorbed on the surface of the gold substrate, and high-throughput detection of crystal violet can be realized by combining a high-throughput rotating detection disk.

[0062] 1) Preparation of gold nanoparticles

[0063] Colloidal gold particles of 40 nm were prepared by trisodium citrate reduction method. Before the experiment, first soak the glassware (round bottom flask, condenser tube and beaker) in aqua regia for 24 hours, then rinse repeatedly with distilled water, and then soak in ultrapure water overnight; take out the vessel and dry it for use; accurately measure 99mL of ultrapure water Pure water was placed in a round bottom flask, and a magnetic stirring rotor was added at the same time; 1 mL of chloroauric acid (mass fraction 1%) was added, and the heating and stirring switch in the heating mantle was turned on at the same time, and 1 mL of trisodium c...

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Abstract

The invention provides a high-throughput detection device and method for rapid analysis of Raman spectrum. The high-throughput detection device for rapid analysis of the Raman spectrum comprises an optical-disc-shaped micropore plate, a rotary slide table and a sealing cover, wherein a plurality of micropores for accommodating samples are arranged on the optical-disc-shaped micropore plate, and the micropores are arranged in a uniform circular array mode from inside to outside on the optical-disc-shaped micropore plate; the optical-disc-shaped micropore plate is connected to the rotary slide table in a threaded mode; and the size of the sealing cover is matched with the size of the optical-disc-shaped micropore plate and is in sealing with the optical-disc-shaped micropore plate. The high-throughput detection method rapid analysis of the Raman spectrum can be widely applied to multi-throughput detection and analysis of food and environmental pollutants. The micropore plate is combinedwith the precision rotary slide table, the qualitative and quantitative analysis of different components are achieved by collecting SERS spectral data of each sampling point without repeatedly adjusting the focal length. The high-throughput rapid analysis of components of a plurality of samples to be detected can be achieved, and rapid on-site detection application is facilitated.

Description

technical field [0001] The invention relates to the field of rapid detection of food and environment, in particular to a high-throughput detection device and a detection method that can be used for rapid and multi-throughput detection of food and environmental pollutants. Background technique [0002] Raman spectroscopy detection technology uses the shift of the frequency of the scattered light and the incident light to correspond to the composition and structure of the material molecules, thus providing rich material structure information. In the actual analysis and detection application process, the Raman signals of most of the targets to be detected are weak, so it is necessary to mix the samples to be tested with Raman enhancing reagents such as nanoscale noble metals (gold, silver nanoparticles) or semiconductor oxides to generate Charge transfer or localized surface plasmon enhancement effects (usually can increase the signal intensity to 6-8 orders of magnitude). [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李攻科霍冰洋胡玉玲
Owner SUN YAT SEN UNIV
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