Bivalent gold-labeled test strip for classical swine fevervirus and porcine pseudorabies virus and preparation method thereof

Abstract

The invention discloses a bivalent gold-labeled test strip for a classical swine fevervirus and a porcine pseudorabies virus and a preparation method thereof. The bivalent gold-labeled test strip comprises a support plate, a sample pad, a gold label pad, an NC membrane and an absorbent pad; the NC membrane contains the imprints of a classical swine fevervirus detection line T1 ''|'', a porcine pseudorabies virus detection line T2 ''|'' and a quality control line C ''|''. During detection, when three red strips ''|||'' appear on a detection membrane, the test strip shows that there is the co-infection of the classical swine fevervirus and porcine pseudorabies virus; when two red strips ''||'' of the detection line T1 and the quality control line C appear, the test strip shows that there isonly the swine fever virus infection; when two red strips ''||'' of the detection line T2 and the quality control line C appear, the test strip shows that there is only the porcine pseudorabies virusinfection; and when only one red strip ''|'' appears, the test strip shows that the result is negative, and both viruses are not infected. The test strip is strong in specificity, high in sensitivity,wide in response spectrum, can detect most existing epidemic isolates, simple and rapid in operation, can be widely used for the detection of classical swine fevervirus infection and porcine pseudorabies virus infection, and is easy to be popularized and applied in production practice.

Description

technical field [0001] The invention relates to a detection device for identifying two kinds of livestock diseases at the same time, in particular to a double gold standard detection test paper for swine fever virus and porcine pseudorabies virus and a preparation method thereof. Background technique [0002] Classical swine fever (CSF) is a highly contagious disease characterized by high fever, hemorrhage and high mortality caused by classical swine fever virus (CSFV). Included in the list of OIE epidemic diseases, it is an animal infectious disease that needs to be declared, and it is listed as a first-class animal epidemic disease in my country. In my country, the hog cholera lapinized vaccine (HCLV) vaccine has been used to prevent and control the large-scale epidemic of CSF. Sexual infection, so-called "atypical swine fever", "mild swine fever" and "venomous sow syndrome" have appeared, so it is still one of the main infectious diseases that endanger the pig industry. C...

AI Technical Summary

Problems solved by technology

At the end of 2011, in Hebei, Henan, Shandong, Shanxi and other provinces, many pig farms immunized with Bartha-K61 vaccine broke out the disease suspected of PR. The affected pigs had high temperature (40-42℃), loss of appetite, cough, diarrhea and accompanied by There are systemic neurological symptoms, and the mortality rate of newborn piglets is high; experiments such as virus isolation have confirmed that the source of the outbreak is the mutated PRV virus, which poses a severe challenge to the prevention and control of PR

At present, there are many methods for CSF and PRV antigen detection, mainly virus isolation, neutralization test and immunohistochemical test, etc. These methods are cumbersome and time-consuming, and are not convenient for large-scale sample detection

Although enzyme-linked immunoassay (ELISA) and RT-PCR methods have high sensitivity and specificity, these detection methods need to rely on a PCR machine or a fluorescent quantitative PCR machine, and there are relatively cumbersome operations, long time-consuming, and detection problems. High cost and other shortcomings, and high technical requirements for equipment and operators, are not suitable for basic laboratory operations, which limits its application in epidemiological investigations and veterinary clinics

However, there is no relevant report on the dual gold standard test paper for classical swine fever virus and porcine pseudorabies virus

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