A test paper for identification and detection of strong and weak strains of Newcastle disease virus

Abstract

The invention relates to a detecting appliance for distinguishing infection and immunization of diseases in poultry, and particularly relates to a test paper for distinguishing a virulent strain from an attenuated strain of a newcastle disease virus. The test paper is composed of a support plate, a sample pad, a gold pad, a detection membrane and an absorbent pad, wherein the detection membrane contains prints of a virulent detection line T1 '|', an attenuated detection line T2 '|' and a quality control line C '|'; when distinguishing detection is carried out, infection of the virulent newcastle disease virus is determined when three red strips '|||' appear on the detection membrane; immunization of a newcastle attenuated vaccine is determined when two red strips '||' appear; and a negative newcastle disease virus is determined when only one red strip '|' appears. The distinguishing detection test paper has the advantages of high specificity, high sensitivity, and wide response spectrum, is simple, convenient and fast to operate, can be widely applied to distinguishing detection of infection and immunization of the newcastle disease virus, and is easy to popularize and apply in production practice; and existing attenuated vaccine strains and most of popular virulent strains can be detected.

Description

technical field [0001] The invention relates to a differential detection instrument for poultry epidemic infection and immunity, in particular to a test paper for differential detection of strong and weak Newcastle disease virus. Background technique [0002] Newcastle disease (ND), also known as Asian chicken plague, is a highly contagious, acute septic avian infectious disease caused by Newcastle disease virus (NDV) with respiratory and gastrointestinal mucosal bleeding as typical lesions. In addition to poultry, at least 200 species of birds can be infected naturally or in laboratories, and it is one of the most serious infectious diseases threatening the poultry industry in the world. Since the disease was first reported in 1926, it is still prevalent in various parts of the world, with outbreaks from time to time, causing serious economic losses to the poultry industry. The World Health Organization (OIE) listed it as a notifiable disease, and my country listed it as a ...

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control of Newcastle disease in my country still adopts a strategy based on vaccine immunization. Since the 1990s, attenuated ND vaccine strains have been frequently used on a large scale and in high doses in my country. Although the virulent epidemic strain can produce complete clinical protection, it cannot prevent the infection, replication and excretion of the epidemic strain in immunized chickens, and ND virulent infection still occurs in immunized chicken flocks. At the same time, due to the lack of serological markers and supporting Differential diagnosis method, its large-scale application in my country makes it difficult to distinguish immune attenuated virus from wild virus infection through antibody detection techniques such as hemagglutination inhibition test (HI) and enzyme-linked immunosorbent assay (ELISA), which is not conducive to the control and purification of ND

With the completion of the complete gene sequences of NDV wild strains and vaccine strains from different sources and regions and the development of molecular biology detection technology, scholars at home and abroad have conducted a lot of research on the identification and detection of NDV. Strain characteristic sites, respectively design strong and weak strain-specific primers or probes, and establish molecular identification detection methods such as RT-PCR and fluorescent quantitative PCR to distinguish strong and weak NDV, with high sensitivity and specificity , but these detection methods need to rely on PCR machines or fluorescent quantitative PCR machines, and have disadvantages such as relatively cumbersome operations, long time-consuming, and high detection costs, which limit their application in epidemiological investigations and veterinary clinics

Images