Hog cholera virus and bovine viral diarrhea virus identification and detection test paper

A technology for bovine viral diarrhea and swine fever virus, which is applied in the field of animal virus identification and detection equipment, can solve the problems of high technical requirements for equipment and operators, unsuitable basic-level laboratory operations, and inconvenience for large-scale sample detection, etc. The effects of wide application, simple and fast operation, and low cost

Inactive Publication Date: 2015-03-25
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for CSF and BVDV antigen detection, mainly virus isolation, neutralization test and immunohistochemical test, etc. These methods are cumbersome and time-consuming, and are not convenient for large-scale sample detection
Although enzyme-linked immunosorbent assay (ELISA) and RT-PCR methods are sensitive, accurate, and fast, they require high technical equipment and operators, and are not suitable for basic-level laboratory operations

Method used

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  • Hog cholera virus and bovine viral diarrhea virus identification and detection test paper
  • Hog cholera virus and bovine viral diarrhea virus identification and detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1, rapid diagnosis of classical swine fever virus and bovine viral diarrhea virus, collecting diseased tissues of sick (dead) pigs and cattle, including lung, liver, spleen, kidney, trachea, small intestine, large intestine and lymph nodes, etc., at a ratio of 1:5 Or add an appropriate amount of PBS or water at 1:10 for simple grinding to prepare the solution to be tested, use the test paper for the identification of swine fever virus and bovine viral diarrhea virus to perform detection and result judgment according to the operation method (14), and differentially diagnose sick (dead) pigs, Bovine CSFV infection or CSFV immunization mixed BVDV infection, CSFV infection or CSFV immunization, BVDV infection. Three reddish-brown bands appear on the test paper (the swine fever virus detection line, the bovine viral diarrhea virus detection line and the quality control line) "|||" are positive for CSFV and BVDV, indicating that the pigs and cattle to be tested are CSF...

Embodiment 2

[0065] Example 2: Inspection and quarantine of pigs and cattle that are healthy in appearance and in febrile phase. Collect swabs (throat swab and anal swab), feces or blood samples in febrile phase from healthy pigs and cattle in febrile phase, and add appropriate amount of PBS or Simply suspend in water, and use test paper for identification of classical swine fever virus and bovine viral diarrhea virus to detect and judge the results according to the operation method (14), to identify CSFV infection of healthy pigs and cattle in febrile phase, or CSFV immune mixed BVDV infection, CSFV infection or CSFV immunity, BVDV infection. Three reddish-brown bands appear on the test paper (the swine fever virus detection line, the bovine viral diarrhea virus detection line and the quality control line) "|||" are positive for CSFV and BVDV, indicating that the pigs and cattle to be tested are CSFV infected or CSFV immune mixture BVDV infection; two reddish-brown bands (the swine fever ...

Embodiment 3

[0066] Example 3, the quality detection of the attenuated swine fever virus rabbitized vaccine (cell source), adding an appropriate amount of PBS or water to dissolve the attenuated swine fever virus rabbitized vaccine (cell source) at 1:5 or 1:10, and preparing the vaccine solution to be tested. Use test paper for identification and detection of classical swine fever virus and bovine viral diarrhea virus according to the method (14) to detect and judge the results, detect the virus content of the attenuated classical swine fever vaccine (cell source) and identify the contamination of bovine viral diarrhea virus in the vaccine . Three reddish-brown bands appear on the test paper (swine fever virus detection line, bovine viral diarrhea virus detection line and quality control line) "|||" are positive for CSFV and BVDV, indicating that the vaccine to be tested is contaminated by BVDV; two reddish-brown stripes The band (test line of swine fever virus and quality control line) "|...

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Abstract

The invention relates to an appliance for identifying and detecting animal viruses belonging to flaviviridae pestiviruses and in particular relates to hog cholera virus and bovine viral diarrhea virus identification and detection test paper. The test paper consists of a supporting plate, a sample pad, a gold-labeled pad, a detection film and an absorbent pad, wherein imprints of a hog cholera virus detection line T1 shaped like a vertical line, a bovine viral diarrhea virus detection line T2 shaped like a vertical line and a quality control line C shaped like a vertical line are arranged on the detection film. During identification and detection, when three red stripes shaped like three vertical lines present on the detection film, the sample is infected with the hog cholera virus or immunized by a hog cholera virus vaccine, and is also infected with the bovine viral diarrhea virus; when two red stripes shaped like two vertical lines present on the hog cholera virus detection line and the quality control line, the sample is infected with the hog cholera virus or immunized by the hog cholera virus vaccine; when two red stripes shaped like two vertical lines present on the bovine viral diarrhea virus detection line and the quality control line, the sample is infected with the bovine viral diarrhea virus; and when only one red stripe shaped like a vertical line presents on the detection film, the sample is negative to the hog cholera virus and bovine viral diarrhea virus. The identification and detection test paper is high in specificity, high in sensitivity, wide in response spectrum and easy and convenient and rapid to operate, can be widely applied to identifying and detecting the hog cholera virus and bovine viral diarrhea virus and is easy to popularize and apply in production practice.

Description

technical field [0001] The invention relates to a device for identifying and detecting animal viruses of the genus Pestivirus in the family Flaviviridae, in particular to a test paper for identifying and detecting swine fever virus and bovine viral diarrhea virus. Background technique [0002] CSFV (Classcal Swine Fever Virus, CSFV) and Bovine Viral Diarrhea Virus (BVDV) belong to members of the Pestivirus genus of the family Flaviridae, and the infectious diseases they cause are very common in pigs and cattle. caused huge economic losses. [0003] Classical swine fever (CSF) is a highly contagious disease caused by classical swine fever virus characterized by high fever, hemorrhage and high mortality. It is listed as an OIE epidemic by the World Organization for Animal Health (OIE) List, which is an animal infectious disease that must be declared, and our country lists it as a first-class animal disease. Swine fever only infects pigs and wild boars. Swine fever virus can ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
CPCG01N33/56983G01N33/558G01N33/577G01N2333/185
Inventor 王丽柴书军杨苏珍孙亚宁杨继飞史西保李青梅郅玉宝赵东梁跃郭军庆杨艳艳邓瑞广张改平
Owner HENAN ACAD OF AGRI SCI
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