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Strain with chromium tolerance and Cr(VI) removal ability and application of the same in in-situ remediation of moderately and mildly chromium contaminated soil

A chromium-contaminated soil, in-situ remediation technology, applied in the restoration of contaminated soil, bacteria, microorganism-based methods, etc., can solve problems such as low crop production, achieve simple operation, easy acceptance and promotion, and ensure successful colonization. Effect

Active Publication Date: 2019-01-11
POMOLOGY INST SHANXI ACAD OF AGRI SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few studies on the use of isolated microbial strains for in situ remediation of chromium-contaminated soils without delaying crop production

Method used

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  • Strain with chromium tolerance and Cr(VI) removal ability and application of the same in in-situ remediation of moderately and mildly chromium contaminated soil
  • Strain with chromium tolerance and Cr(VI) removal ability and application of the same in in-situ remediation of moderately and mildly chromium contaminated soil
  • Strain with chromium tolerance and Cr(VI) removal ability and application of the same in in-situ remediation of moderately and mildly chromium contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the separation of cellulosic bacteria Cr8

[0020] Soil was collected from an abandoned chromium salt factory in Jinzhong City, Shanxi Province (N37°34′34.01, E113°43′42.06), and the collected fresh soil was fully mixed and cultured with “selective medium + dilution coating” method for isolating bacteria. Weigh 10g of soil into a sterile Erlenmeyer flask, add 100mL of sterile water, and shake for 30 minutes to obtain 10 -1 Soil suspension, draw 1mL with a sterile pipette tip in an ultra-clean bench 10 -1 Soil suspension into 9mL sterile water, that is, 10 -2 Soil suspension was prepared in this way until 10 -8 soil suspension. Draw 200 μL of the soil suspension of -6, -7, and -8 dilution gradients and apply it in the solution containing 1000 μg / mL K 2 Cr 2 o 7 cultured in the dark at 30°C for 3 days, and obtained a strain with fast growth and large colonies, which was purified by streaking until a pure culture was obtained, and a single colony was ...

Embodiment 2

[0021] Embodiment 2: the identification of cellulosic bacteria Cr8

[0022] Morphological characteristics: For the colony characteristics of the strain numbered Cr8 on the LB culture plate, see figure 1 , is: round, yellow, with a diameter of about 1 mm, a protrusion in the middle, and a moist surface. Observe the morphology of Cr8 cells after simple staining, see figure 2 , found that the bacteria were rod-shaped or club-shaped, without spores. In addition, some physiological and biochemical indicators of Cr8 are shown in Table 1.

[0023] Table 1 Physiological and biochemical indicators of strain Cr8

[0024] index

result

index

result

index

result

s

+

Nitrate reduction

+

maltose

-

Produce IAA experiment

-

Xylitol

-

Galactose

+

Cellulose degradation

-

Sorbitol

-

Oxidase assay

-

gelatin liquefaction

+

sucrose

+

Catalase

+

starch hydrolysis

...

Embodiment 3

[0027] Embodiment 3: Determination of chromium-resistant ability of cellulosic bacteria Cr8:

[0028] Determination method: Pick a single Cr8 colony, insert it into LB liquid medium, and culture it on a shaker at 30°C and 180 rpm for 24 hours to make a seed solution. After centrifuging and discarding the supernatant, add LB liquid medium to adjust its OD value to 0.5, and add K 2 Cr 2 o 7 LB liquid medium, K 2 Cr 2 o 7 Concentrations were set at 4 gradients of 0, 300, 500 and 1 000 μg / mL, and cultured on a shaking table at 30 °C and 180 rpm for 2 h, 4 h, 8 h, 12 h, 24 h, 48 h and 72 h. Measure its OD value at a wavelength of 600 nm, see image 3 .

[0029] From image 3 It can be seen that Cr8 can grow very well in the chromium-containing medium, and in both the chromium-containing and chromium-free medium, it enters the logarithmic growth phase when it is cultivated for 12H, and enters the stable growth phase when it is cultivated for 48H. Low concentration of chro...

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Abstract

The invention belongs to the technical field of microbial remediation of contaminated soil, and provides a strain with chromium tolerance and Cr(VI) removal ability and an application of the same in in-situ remediation of moderately and mildly chromium contaminated soil. The strain is Cellulosic Microbium sp. CGMCC NO: 16135, deposited in the General Microbial Center of China Microbial Culture Collection Management Committee on July 19, 2018. It is chromium-resistant and chromium-removing. Cellulose microorganisms are first found to have high chromium tolerance and hexavalent chromium removalcapacity in China. Mixed with wheat bran, chitosan and peat soil, the microbial inoculum is beneficial to the survival and rapid multiplication of bacteria, and brown sugar water ensures the successful colonization of functional bacteria. The decomposed organic fertilizer is beneficial to the growth and multiplication of bacteria and the removal of hexavalent chromium. When it is applied to the remediation of chromium contaminated farmland, the fertilization and detoxification are carried out at the same time, and the operation is simple and easy to be accepted and popularized.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of polluted soil, and specifically relates to a bacterial strain with chromium tolerance and Cr(VI) removal ability and its application in in-situ remediation of slightly chromium-contaminated soil. Background technique [0002] my country's chromium salt industry started in 1958. At present, my country's annual chromium salt production capacity exceeds 350,000 tons, and it has become the world's largest producer and consumer of chromium salt. However, the production of chromium salts produced a large amount of chromium slag. According to the statistics of the National Development and Reform Commission and the former State Environmental Protection Administration in 2012, the cumulative production of chromium salts in the country was more than 2 million tons, and more than 6 million tons of chromium slag were produced, of which only about 2 million tons have been disposed of, and ther...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10B09C2101/00C12N1/20C12N1/205C12R2001/01
Inventor 肖蓉聂园军曹秋芬王媛李建军张强郜春花赵佳薄晓峰
Owner POMOLOGY INST SHANXI ACAD OF AGRI SCI
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