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Application of total astragaloside to anti-photoaging and photodamage of skin

A technology of skin photoaging and total astragalosides, which is applied in skin care preparations, applications, skin diseases, etc., can solve the problems of no research reports on total astragalosides, toxic and side effects, etc., and achieve the goal of promoting collagen accumulation and rich sources of raw materials Effect

Inactive Publication Date: 2018-12-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no research report on the effect of total astragalosides on improving skin photoaging
Moreover, some of the currently used products for the prevention and treatment of photoaging are accompanied by relatively large toxic and side effects, so various research institutions and companies are actively developing new products

Method used

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  • Application of total astragaloside to anti-photoaging and photodamage of skin
  • Application of total astragaloside to anti-photoaging and photodamage of skin
  • Application of total astragaloside to anti-photoaging and photodamage of skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and Culture of Rat Dermal Fibroblasts (RDFs)

[0037] Select Sprague-Dawley suckling mice (SD suckling mice) within 3 days after birth, regardless of sex, for the extraction and culture of primary skin fibroblasts.

[0038] (1) Take 1-3 day old SD suckling mice, place them in a petri dish, put the petri dish on ice, immerse the sacrificed mice in povidone iodine for 2 minutes, repeat 2 times. Then immerse the suckling mouse in deionized water to remove iodophor, then continue to immerse the suckling mouse in 70% ethanol for 2 minutes, repeat twice, and then place the petri dish on ice.

[0039](2) Use tweezers and scissors to first cut off the limbs and tail of the suckling mouse, and then slowly and carefully cut the skin along the gap of the tail and along the direction of the spine with scissors. Be careful not to cut the internal organs too hard and cause massive bleeding. Cut to the back of the head , Use tweezers to hold the skin on both sides and tear ...

Embodiment 2

[0050] Identification of rat dermal fibroblasts

[0051] The primary dermal fibroblasts were inoculated in a special dish for laser confocal, added to DMEM containing 10% FBS and placed in a 5% CO2 cell incubator for 24 hours. When the cells grow to about 70%-80% confluence, the medium is discarded, and the normal temperature PBS buffer is gently washed 2-3 times. Aspirate the PBS, add an appropriate amount of 4% paraformaldehyde to the culture dish until the cells are completely covered, and fix the cells at room temperature for 30 minutes. Discard the paraformaldehyde, wash gently with room temperature PBS buffer 2-3 times, place the petri dish on a decolorizing shaker and shake for 5 minutes each time. Aspirate the PBS liquid, add an appropriate amount of 1% Triton-X100 PBS solution that completely covers the cells, and rupture the membrane for 30 minutes at room temperature. Discard the 1% Triton-X 100PBS solution, wash gently with PBS buffer at room temperature for 2-3 ...

Embodiment 3

[0053] UVB irradiation

[0054] Count the cells and inoculate them into 6-well plates or small 10mm petri dishes according to a certain number. After the cells are cultured in a cell culture incubator at 5% CO2 and 37°C for more than 12 hours, when the cell density is about 30%, take out the culture. Put the portable UVB irradiation lamp into the ultraviolet sterilization in advance, adjust the height of the UV lamp, turn on the light source, and keep the output light intensity stable. Right below the UVB lamp, use The UVB irradiator detects the radiation intensity at the height of the petri dish, calculates the irradiation time of different doses according to the irradiation dose (mJ) = irradiation intensity (mW) × time (s), and then removes the petri dish or culture plate cover , placed under a UVB light source, the irradiation doses are 0mJ, 1mJ, 2mJ, 3mJ, 4mJ, 5mJ, if the hole or dish is not in the irradiation, use aluminum foil to shield the UVB, after the irradiation, pu...

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Abstract

The invention relates to application of total astragaloside to anti-photoaging and photodamage of skin. The total astragaloside can improve the photoaging caused by long-term repeated irradiation of UVB (Ultraviolet B). As proved by cell level experiments, the total astragaloside has good effects in anti-oxidation, anti-photodamage, anti-photoaging, protection of mitochondria and inhibition of apoptosis induced by the UVB and the like. Meanwhile, the total astragaloside can also reverse the loss process of collagen in skin cells during photoaging, and promote the accumulation of collagen. On the other hand, the autophagy activity of photoaging cells can be up-regulated, and the normal cell homeostasis process is maintained so as to better resist the photoaging process. The total astragaloside is proved to have a good effect in the anti-skin photoaging process, and can be applied in the fields of pharmaceutical, health care products, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical fields of medical technology, health products and cosmetics, and specifically relates to the use of total astragalosides in resisting skin photoaging. Background technique [0002] The skin is the largest organ of the human body exposed to the outside world, so human aging is first manifested in the aging of the skin, such as increased skin wrinkles, sagging skin, etc. Skin photoaging is the aging caused by DNA changes and skin damage caused by exogenous factors such as ultraviolet radiation, exposure to environmental toxins, wind and smoking. Among them, long-term repeated exposure to ultraviolet rays in the sun is the most important factor in the environment factors affecting skin aging. [0003] The mechanism of photoaging has not yet been fully elucidated, but free radicals are considered to be the most important factor in the occurrence and development of photoaging. Excessive accumulation of free radicals ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q17/04A61Q19/08A61K36/481A61P39/06A61P17/00A23L33/105
CPCA23L33/105A23V2002/00A61K8/9789A61K36/481A61P17/00A61P39/06A61Q17/04A61Q19/08A23V2200/30A23V2250/21
Inventor 李民刘培庆温炜杰陈健文
Owner SUN YAT SEN UNIV
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