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CRISPR/Cas9-based sgRNA design method

A design method and technology for designing models, applied in the field of gene editing research, can solve problems such as poor prediction performance and low model evaluation accuracy, and achieve the effects of improving evaluation accuracy, expanding the scope, and widening the application scope.

Inactive Publication Date: 2017-02-22
TONGJI UNIV
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Problems solved by technology

Both types of software build a general model. However, due to the large heterogeneity between different species and different cells, the prediction performance of existing software is not very good, and because different experimental conditions The heterogeneity of sgRNA has a certain impact on the digestion efficiency of sgRNA, and the general model evaluation accuracy is relatively low

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  • CRISPR/Cas9-based sgRNA design method
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  • CRISPR/Cas9-based sgRNA design method

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Embodiment Construction

[0058] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is carried out on the premise of the technical solution of the present invention, and detailed implementation and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.

[0059] Explanation of acronyms:

[0060] CRISPR: Clustered regularly interspaced short palindromic repeats

[0061] clustered regularly interspaced small palindromic repeats

[0062] Cas9: an enzyme related to the CRISPR type II system

[0063] NGS: Next Generation Sequencing, next generation sequencing

[0064] PAM: Protospacer-adjacent motif, a marker fragment necessary for sgRNA to recognize DNA

[0065] sgRNA: RNA that acts as a guide in the CRISPR / Cas9 system

[0066] indel: insertions and deletions on DNA caused by CRISPR / Cas9 editing

[0067] spacer: About 20 bases in the...

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Abstract

The invention relates to a CRISPR / Cas9-based sgRNA design method. The method is characterized by comprising the following steps of obtaining the value of enzyme digestion efficiency of sgRNA and corresponding Cas9; building a personalized sgRNA design model; measuring the quality of the built personalized sgRNA design model by applying an NDCG algorithm and updating a database; and designing sgRNA and giving out an assessment value of each sgRNA. Compared with the prior art, the method has the characteristics of high accuracy, complete characteristics, wide application range and wide data analysis range.

Description

technical field [0001] The invention relates to the field of gene editing research, in particular to a method for designing sgRNA based on CRISPR / Cas9 gene editing technology. Background technique [0002] With the development of molecular biology, people have a deeper understanding of the constituent elements of life, but there are still many puzzles about the mechanism of life processes, especially the healing mechanism of certain diseases. The relationship between genes and phenotypes, and the mutual influence between genes, urgently require an engineering technology that can quickly knock out and insert genes in vivo. The CRISPR / Cas9 system appeared in time to meet the needs of scientific researchers. [0003] The CRISPR / Cas9 system (Clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) is a gene editing tool with simple operation and wide applicability. The whole system is mainly composed of a nuclease (Cas9) and an RNA (sgRNA) that gu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/12
Inventor 刘琦啜国晖陈亚男闫纪芳
Owner TONGJI UNIV
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