Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of human dental pulp stem cell culture supernatant to preparation of cosmetic

A technology of dental pulp stem cells and culture supernatant, which is applied to the application field of human dental pulp stem cell culture supernatant in the preparation of cosmetics, and achieves the effects of preventing wrinkles, simply taking materials, and maintaining skin elasticity.

Inactive Publication Date: 2018-06-29
常方圆
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report about the application of dental pulp stem cell culture supernatant in cosmetics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of human dental pulp stem cell culture supernatant to preparation of cosmetic
  • Application of human dental pulp stem cell culture supernatant to preparation of cosmetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Obtaining the culture supernatant of human dental pulp stem cells:

[0018] Choose deciduous teeth that have fallen out naturally, orthodontics, permanent teeth extracted by oral surgery, etc., and must be teeth with vital pulp without any lesions. After the tooth was pulled out, a groove of a certain depth was milled around the neck of the tooth at the enamel-cementum junction with a turbine drill (be careful not to penetrate the pulp), and then rinsed repeatedly with PBS buffer containing penicillin / streptomycin. The tooth was split along the groove in the ultra-clean bench, and the pulp was removed under aseptic conditions. Transfer to a centrifuge tube containing 1 mL of phosphate buffered saline, cut to 1 mm with sterile scissors 3 size, add 500uL type I collagenase, digest in a 37°C incubator for 60 minutes, and blow it with a gun every 15 minutes. Add 3 times the volume of phosphate buffer to stop the digestion, put it in a centrifuge at 1000 rpm, centrifuge fo...

Embodiment 2

[0021] An essence emulsion containing human dental pulp stem cell culture supernatant, which contains the following ingredients by mass percentage:

[0022] Component A: Isononyl Isononanoate 2%

[0023] Tocopheryl Acetate 0.4%

[0024] Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 1.3%

[0025] Dimethiconol 2%

[0026] Cyclopentasiloxane / Dimethicone Crosspolymer 1.5%

[0027] Dimethicone 2%

[0028] Component B: deionized water 58.71%

[0029] Glycerin 10%

[0030] 1,3-Butanediol 7%

[0031] Hyaluronic Acid 0.03%

[0032] Carbomer 0.15%

[0033] EDTA 0.05%

[0034] Allantoin 0.2%

[0035] Component C: Triethanolamine 0.15%

[0036] Tripeptide-1 3%

[0037] Lotus flower extract 1%

[0038] Methylisothiazolinone 0.5%

[0039] Fragrance 0.01%

[0040] D component: human dental pulp stem cell culture supernatant 10%

[0041] Process: heat and mix components A and B separately, then homogeneously emulsify, then cool to 37-50°C, add components C an...

Embodiment 3

[0043] Detection of anti-aging performance of human dental pulp stem cell culture supernatant:

[0044] Human dermal fibroblasts were isolated from human foreskin tissue. The experiment was divided into 4 groups to measure the proliferation rate of human dermal fibroblasts, respectively adding 0%, 5%, 10% and 20% volume fraction of human dental pulp stem cell culture supernatant to normal medium. The specific method is: divide the cells into 5×10 4 Inoculate at a density of 96 wells per ml and culture continuously for 24 hours, 48 ​​hours, 72 hours, and 96 hours, discard the medium, add 100 μl of CCK8 solution, continue to cultivate for another 3 hours, and then detect the absorbance value at a wavelength of 450 nm (absorbance Values ​​reflect cell numbers). The experiment was repeated three times.

[0045] The experimental results showed that after adding human dental pulp stem cell culture supernatant, the absorbance value was significantly higher than that of the control...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a human dental pulp stem cell culture supernatant to preparation of a cosmetic. Human dental pulp stem cells are obtained through isolation from human dental pulp and in-vitro culture; the culture supernatant is a cell secretion-containing culture medium collected during cell culture; the human dental pulp stem cell culture supernatant, as an active componentwith a fibroblast proliferation promoting effect, is applied to the preparation of the cosmetic. The human dental pulp stem cell culture supernatant is added into the cosmetic by a homogenizing technology, and the content of the human dental pulp cell culture supernatant in per gram of the cosmetic is 0.05-0.8g. The human dental pulp stem cell culture supernatant, as a bioactive component, is added into the cosmetic, so that the skin elasticity can be effectively maintained, wrinkles can be reduced and prevented, and the skin health is kept.

Description

technical field [0001] The present invention relates to the use of human dental pulp stem cells, in particular to the application of human dental pulp stem cell culture supernatant in the preparation of cosmetics. Background technique [0002] Cell aging is the root cause of human aging. With the increase of age, the physiological functions of the human body gradually decline, and there are more aging cells than new ones. The most obvious external manifestation of human aging is the skin. As we age, the skin begins to wrinkle, sag, pigmentation, and lose luster. The pathophysiological changes of skin aging originate from both epidermis and dermis. However, because the skin dermis contains various types of cells and criss-cross extracellular matrix components, it plays a leading role in the skin aging process. Fibroblasts are the most important cell components in the dermis, and the gradual aging of their proliferation ability is one of the important reasons leading to ski...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K8/99A61Q19/08
CPCA61K8/99A61Q19/08
Inventor 常方圆杨佳音李月玲
Owner 常方圆
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com