Preparation method of hydrolyzed protein and chitin

A technology of hydrolyzed protein and hydrolyzed protein powder, which is applied to the preparation method of peptides, chemical instruments and methods, fat oil/fat refining, etc., which can solve the problems of backward processing technology, red tide, high operation cost, etc., and achieve easy control of process steps, process Reasonable design route and good product quality

Inactive Publication Date: 2018-06-26
刘明明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the utilization of crustacean processing wastes has the following problems: backward processing technology, single product (only chitin), high cost, resulting in waste of resources, and a large number of strong acids and alkalis have caused serious pollution, resulting in high concentration, high Salinity organic wastewater whose polluting components include large amounts of CaCl 2 , NaCl and other salts, a large amount of animal protein (shrimp, crab shell protein) and its degradation products, shrimp (crab) shell pigment, oil, impurities brought by the raw material itself, a small amount of chitin and chitosan, etc.
Untreated water is black, smelly, and eutrophic, causing serious pollution to aquaculture and water environment, and can even induce red tides
At present, in the treatment of chitin production sewage, a simple terminal treatment method is adopted, which requires a large one-time investment, high operating costs, and it is difficult to achieve discharge standards
There are hundreds of chitin processing industries in my country's coastal areas. Due to the limited consumption of chitin in China, a large number of products still rely on exports to make profits. However, the disorderly competition of a large number of manufacturers makes the chitin industry profitable, and some are in trouble.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Prepare the product with shrimp shells as the raw material. First, dry the shrimp shells at 85-90° C., then crush the dried shrimp shells to 40-80 mesh, put them into the enzyme-cutting tank, and put them in the enzyme-cutting tank. Add 3.5 times of water and stir for 30-60 minutes, let stand at room temperature to swell for 1-2 hours; then, heat the enzymatic cutting tank to 51-55°C, according to the weight of the input shrimp shell powder raw material, the weight percentage is 0.6% For example, add mixed enzymes, adjust the pH value to 6.5-7.5, stir for 2 to 4 hours, and filter with a plate and frame filter press to obtain the refined enzyme-cut filtrate and clean shrimp shell powder; centrifuge the refined enzyme-cut filtrate, The upper liquid is crude oil, and the remaining liquid in the lower layer is hydrolyzed protein liquid. After decolorization and second fine filtration, it is desalted on a Sephadex G-10 column, then concentrated in a vacuum and spra...

Embodiment 2

[0014] Embodiment 2: Step and method are identical with embodiment 1, take crab shell as raw material, and its product quality is identical.

Embodiment 3

[0015] Embodiment 3: The steps and method are the same as in Embodiment 1, using the mixed powder of shrimp and crab shells as raw material, and the product quality is the same.

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PUM

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Abstract

The invention relates to a preparation method for processing hydrolyzed protein and chitin by using animal shell as a raw material. The method comprises: drying the shell of shrimp or crab, crushing to achieve a particle size of 40-80 mesh, pouring into an enzyme digestion tank, adding water, stirring, and carrying out standing swelling at a room temperature; heating the enzyme digestion tank to atemperature of 51-55 DEG C, adding mixed enzyme accounting for 0.6% of the weight of the shrimp shell powder raw material, adjusting the pH value to 6.5-7.5, carrying out stirring and enzyme digestion, and carrying out pressure filtration to obtain an enzyme digestion fine filtrate and pure shrimp shell powder; carrying out centrifugal separation on the fine filtrate, carrying out decoloration and secondary fine filtration on the lower-layer remaining liquid, desalting, and carrying out vacuum concentration and spray drying to obtain a hydrolyzed protein powder product; and adding a hydrochloric acid solution to the pure shell powder, carrying out soaking decalcification, adding sodium hydroxide to achieve a neutral pH value, filtering, washing, and drying to obtain chitin. According to the present invention, the preparation method has characteristics of reasonable process route, easily-controlled process, high production efficiency, good product quality, low environment pollution andlow cost, and is suitable for industrial production.

Description

Technical field: [0001] The invention relates to a clean preparation technology for producing hydrolyzed protein and chitin by using crustacean processing waste, especially the shell matter thereof, as raw materials, in particular to a preparation method for hydrolyzed protein and chitin. Background technique: [0002] As we all know, aquatic animal protein has high nutritional value. A large number of animal experiments have shown that: aquatic animal protein has obvious immune regulation, anti-fatigue, anti-radiation, liver protection, etc., and the hydrolyzed protein produced by biological methods is easier to be absorbed and utilized by the human body, and has good water solubility and water retention. It is widely used in food, medicine and other industries, and has high economic value. Our country is rich in crustacean resources, the amount of breeding and processing of crustaceans is huge, and there is a large amount of processing waste at the same time. The waste of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16C08B37/08C11B1/02C11B3/00
CPCC12P21/06C07K1/16C08B37/0003C08B37/003C11B1/02C11B1/025C11B3/00
Inventor 尤世元马晶晶孙亮
Owner 刘明明
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