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Preparation method of red blood cell membrane-coated gelatin-loaded berberine hydrochloride gold nanoparticles and application thereof

A technology of berberine hydrochloride and red blood cell membrane, which is applied in the directions of medical preparations with non-active ingredients, medical preparations containing active ingredients, capsule delivery, etc. problem, to achieve the effect of high average drug loading, high average encapsulation efficiency and good stability

Active Publication Date: 2018-06-05
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These nano dosage forms of BH have improved the problem of drug administration of BH to a certain extent, but they still cannot solve the problem of excessive peak blood drug concentration caused by the lack of sustained release of BH ordinary injection, so it is difficult to overcome the vasodilation, blood pressure drop, heart failure caused by it. Inhibition and other side effects

Method used

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  • Preparation method of red blood cell membrane-coated gelatin-loaded berberine hydrochloride gold nanoparticles and application thereof
  • Preparation method of red blood cell membrane-coated gelatin-loaded berberine hydrochloride gold nanoparticles and application thereof
  • Preparation method of red blood cell membrane-coated gelatin-loaded berberine hydrochloride gold nanoparticles and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Preparation of erythrocyte membrane RBCM

[0065] (1) Get SD rats, weigh their body weight and record;

[0066] (2) Rats were injected intraperitoneally with 7% chloral hydrate solution, and the rats were anesthetized with an amount of 0.7mL / 100g until the turning reflex disappeared;

[0067] (3) After the rats were fully anesthetized, the rats were dissected to obtain blood by heart puncture, and the blood taken out was stored in an ice box;

[0068] (4) Take a 0.5mL EP tube, add 10μL of 5mg / mL sodium heparin solution to it, keep away from light, add 250μL of blood to each tube, and shake it to fully mix with the sodium heparin. Centrifuge at 3800rpm and 4°C for 10min;

[0069] (5) Remove the supernatant and white blood cell layer in (4), add 1mL 1×PBS, mix thoroughly, and centrifuge at 3800rpm, 4°C for 10min;

[0070] (6) Repeat (5) step 1 to 2 times;

[0071] (7) Remove the supernatant buffy coat in (6), add 900 μL 2mmol / L EDTA solution, mix well, then ...

Embodiment 2

[0075] Preparation of Example 2 RBCM-BH-GNPs

[0076] One, the preparation of the first group of preparations:

[0077] (1) Weigh gelatin solid particles (the gel strength of the gelatin is 250g Bloom), add ultrapure water, stir at room temperature for 30 minutes to swell, then heat to dissolve in a water bath at 50°C; filter the obtained solution and add 0.2 The NaOH solution of M adjusts the pH=6.0, and finally obtains a clear gelatin aqueous solution with a mass fraction of 0.5%;

[0078] (2) Take out a glutaraldehyde solution with a mass fraction of 50% under the storage condition of -20°C, and dissolve it at room temperature; add 4 times the volume of ultrapure water to the above solution, dilute it five times, and obtain a glutaraldehyde solution with a mass fraction of 10%. dialdehyde solution;

[0079] (3) Weigh the solid particles of sodium metabisulfite, add ultrapure water to dissolve, and obtain a sodium metabisulfite solution with a mass fraction of 1.6%, and st...

Embodiment 3

[0125] Example 3 Stability investigation of RBCM-BH-G NPs

[0126] The prepared RBCM-BH-GNPs and BH-GNPs were stored at room temperature, and the particle size and polydispersity index were recorded continuously with a Malvern laser particle size analyzer. BH-GNPs were continuously measured for 30 days, and RBCM-BH-GNPs were measured for 7 days. particle stability. Depend on figure 2 It can be seen that the first group of preparations, BH GNPs (recorded as No. 1-1 preparations) have good stability within 30 days, and RBCM-BH-GNPs (recorded as No. 1-2 preparations) have good stability within 7 days. Stability, PDI remains below 0.15. Similarly, the second group of preparations, BH GNPs (recorded as No. 2-1 preparation) all had good stability within 30 days, and RBCM-BH-GNPs (recorded as No. 2-2 preparation) all had good stability within 7 days. Stability, PDI remains below 0.15. The third group of preparations, BH GNPs (recorded as No. 3-1 preparation) all have good stabil...

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Abstract

The invention belongs to the technical field of a pharmaceutical, and particularly relates to an red blood cell membrane-coated gelatin-loaded berberine hydrochloride gold nanoparticles, as well as apreparation method and application thereof. The RBCM-BH-GNPs prepared through the method is uniform in particle size distribution and favorable in stability, and shows a remarkable shell-core structure; a single-layer erythrocyte membrane coats spherical BH-GNPs. The RBCM-BH-GNPs cannot cause erythrocytes hemolysis or agglutination in vitro, and can be used for intravenous injection. In addition,the RBCM-BH-GNPs prepared through the invention has a remarkable in vitro sustained release effect on BH in vitro and the sustained release effect is superior to the BH-GNPs, the RBCM-BH-GNPs solves the problems of hemangiectasis, fall of blood pressure, heart inhibition and side reaction caused by excessive plasma drug peak concentration since BH common injection is short of slow release, and provides an idea for expanding clinical application of the BH and developing new traditional Chinese medicine preparations of the BH.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a red blood cell membrane-wrapped gelatin-loaded berberine hydrochloride nanoparticle and a preparation method and application thereof. Background technique [0002] Berberine hydrochloride (BH), as an antidiarrheal over-the-counter drug, has few adverse reactions in clinical use, and is the drug of choice for the treatment of gastroenteritis, bacillary dysentery and other diseases. As an active ingredient of traditional Chinese medicine, the safety of BH has been guaranteed for a long time. Modern pharmacological studies have found that BH also has anti-malarial, anti-hypertensive, anti-hyperlipidemic, anti-hyperglycemic, anti-arrhythmic, anti-tumor and anti-inflammatory effects. Although BH has a good curative effect in clinical research, it has poor water solubility and short half-life. After ordinary injection, it quickly enters organs and tissues, and it is eas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/46A61K31/4375A61P35/00A61P31/10A61P31/04A61P9/12A61P3/10A61P3/06A61P9/06
CPCA61K9/0019A61K9/5169A61K9/5176A61K31/4375
Inventor 邱明丰苏靖张然张秀梅郭朋程
Owner SHANGHAI JIAO TONG UNIV
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