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Preparation and application method for injectable temperature-sensitive magnetic supramolecular gel

A supramolecular gel and magnetic technology, applied in biological and medical nanomaterials and fields, can solve the problems of ineffective adjuvant therapy, easy recurrence and metastasis, and low survival rate, and achieve good shape, uniform size, and prolonged survival time Effect

Active Publication Date: 2018-05-29
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coupled with little effect of adjuvant therapy, easy recurrence and metastasis after surgery is one of the main reasons for the low survival rate

Method used

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  • Preparation and application method for injectable temperature-sensitive magnetic supramolecular gel
  • Preparation and application method for injectable temperature-sensitive magnetic supramolecular gel
  • Preparation and application method for injectable temperature-sensitive magnetic supramolecular gel

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Experimental program
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preparation example Construction

[0034] The main steps of the preparation method of the injectable temperature-sensitive magnetic supramolecular gel of the present invention are: adding α-cyclodextrin and water-soluble antineoplastic drug doxorubicin hydrochloride into ultrapure water, vortex mixing, and ultrasonic heating to make it Completely dissolve to form a solution one; use distearoylphosphatidylacetamide-methoxypolyethylene glycol (DSPE-mPEG2000) to carry out water phase transfer to the oil-soluble ferric oxide nanoparticles prepared by high temperature pyrolysis method, and at the same time Load the fat-soluble antineoplastic drug paclitaxel and the fat-soluble fluorescent molecule Cy7 that can observe the degradation process of the gel to obtain solution 2; mix solution 1 and solution 2 at a certain ratio, stir evenly, and stand at room temperature to obtain a magnetic supramolecular gel .

[0035] Wherein, the concentration of α-cyclodextrin is 100-400 mg / mL, and the concentration of the water-solu...

Embodiment 1

[0038] Preparation of Ferric Oxide Nanoparticles and Its PEG Modification by High Temperature Pyrolysis

[0039] Add 20mmol of iron acetylacetonate, 100mL of reaction solvent dibenzyl ether, and 60mmol of surfactant oleic acid into a 500ml slanted three-neck flask, continuously feed nitrogen to remove oxygen in the system and play a stirring role, and condense and reflux at the same time. The temperature is programmed to heat the reaction system at a heating rate of 3.3°C / min to the nucleation temperature of 220°C, and keep the temperature for 1h. This stage is the nucleation stage of magnetic iron oxide nanoparticles. During this process, the solution turns from brownish red to transparent. black. Next, the reaction system was heated to a growth temperature of 290° C. at a heating rate of 3.3° C. / min, and the reaction system was maintained at this temperature for 30 minutes to end the reaction. After the reaction, remove the heat source, transfer the reactant to a beaker aft...

Embodiment 2

[0041] Preparation of Magnetic Supramolecular Gels

[0042] Add 1 mL of the PEG-modified magnetic nanoparticle solution prepared in Example 1 into 2 mL of cyclodextrin aqueous solution, wherein the concentration of DSPE-mPEG2000 is 100 mg / mL, and the concentration of iron element is 2 mg / mL, which is a fat-soluble antitumor The drug paclitaxel concentration is 1 mg / mL, the fat-soluble fluorescent molecule Cy7 concentration is 200 μg / mL, the water-soluble antineoplastic drug doxorubicin concentration is 2 mg / mL, and the cyclodextrin concentration is 200 mg / mL. The obtained mixed solution was vortex stirred evenly, and then left to stand at room temperature for 30 min to obtain a magnetic supramolecular gel.

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Abstract

The invention discloses a preparation and application method for injectable temperature-sensitive magnetic supramolecular gel. The preparation method comprises the following steps that 1, alpha-cyclodextrin and a water-soluble antineoplastic drug are added into ultrapure water, vortex mixing is carried out, and ultrasonic heating is carried out to enable the alpha-cyclodextrin and the water-soluble antineoplastic drug to be completely dissolved so as to form a solution I; 2, aqueous phase transfer is carried out on oil-soluble ferroferric oxide nano-particles prepared by a high-temperature thermal decomposition method by using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methox (polyethylene glycol)-2000] (DSPE-mPEG2000), and meanwhile, a fat-soluble antineoplastic drug is loaded soas to obtain a solution II; and 3, the solution I and the solution II are mixed and are uniformly stirred, and standing is carried out at room temperature so as to obtain the magnetic supramolecular gel. The gel has the shear-thinning-injectable and phase-transition-temperature-adjustable properties, and the hierarchical structure of the gel can be loaded with hydrophilic and hydrophobic diphasicantineoplastic drugs; and residual tumors can be eliminated by the gel from the three aspects of uniform and staged drug release in tumor regions for a long time and cell thermal therapy and heat-promoted local high-concentration drug release caused by heat mediated by the magnetic nano-particles in the phase change conformal process.

Description

technical field [0001] The invention belongs to the field of biological and medical nanomaterials and technologies, and in particular relates to a preparation method and application of an injectable temperature-sensitive magnetic supramolecular gel. Background technique [0002] As an important new smart gel material, supramolecular gels have broad application prospects in drug delivery, tissue engineering, and biosensing. Supramolecular gels are organic small molecules in a specific solvent through intermolecular hydrogen bond interactions, π-π stacking interactions, electrostatic interactions, van der Waals interactions, dipole-dipole interactions, coordination interactions, etc. A three-dimensional network structure formed by the self-assembly of valence bonds. Supramolecular gels can be classified in many ways, and can be divided into organogels, hydrogels and ionogels according to the different solvents fixed. Compared with traditional covalent polymer gels, supramole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K41/00A61K47/69A61K47/24A61K31/704A61K31/337A61P35/00
CPCA61K9/0009A61K9/0019A61K9/06A61K31/337A61K31/704A61K41/0028A61K47/24A61K2300/00
Inventor 张宇武昊安马明顾宁
Owner SOUTHEAST UNIV
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