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Preparation method of patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion

A technology of male reproduction and carotene, which is applied in food science, the function of food ingredients, and food ingredients as emulsifiers, etc., can solve the problems of toxic substances with bad flavor, decreased bioavailability, and decreased biological activity, so as to improve digestion and absorption The utilization rate, the preparation method are simple and easy, and the effect of good stability

Inactive Publication Date: 2018-05-18
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] β-carotene usually accelerates its degradation under the action of light, heat, enzymes, metal ions, metal-binding proteins and microorganisms, causing a series of oxidation reactions, resulting in reduced biological activity, color degradation, bad flavor and potential Toxic substances, decreased bioavailability, etc.

Method used

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  • Preparation method of patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion
  • Preparation method of patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion

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preparation example Construction

[0035] The present invention provides a method for preparing a β-carotene emulsion of the enzymatic hydrolysate of the scallop scallop male gonads. The male gonads of the scallop scallop and β-carotene are prepared, and the preparation steps are as follows:

[0036] S1: Use trypsin to enzymolyze the male gonads of the scallop scallop to obtain an enzymatic hydrolysate with a degree of hydrolysis of 1%, which is freeze-dried for later use;

[0037] S2: Heat β-carotene at 50-55°C for 10-20 minutes to dissolve it in corn oil and prepare it into an oil phase for later use;

[0038] S3: Mix the enzyme hydrolysate peptide solution with a concentration of 10-30 mg / mL, sodium azide stock solution (0.01-0.02wt%) and Tween 20 (0.5-1wt%), and prepare the aqueous phase for use;

[0039] S4: Mix the oil phase and the water phase in a ratio of 1:9, and then disperse at high speed for 2-3min at 10000-15000r / min after mixing;

[0040] S5: Homogenize 5-7 times under high pressure of 10000-12000psi.

Embodiment 1

[0042] A method for preparing a β-carotene emulsion of the enzymatic hydrolysate of the scallop male gonads, comprising the male gonads of the scallop scallop and β-carotene. The preparation steps are as follows:

[0043] S1: Use trypsin to enzymolyze the male gonads of the scallop scallop to obtain an enzymatic hydrolysate with a degree of hydrolysis of 1%, which is freeze-dried for later use.

[0044] S2: Heat β-carotene (0.1wt%) at 50°C for 20 minutes to dissolve it in corn oil and prepare it into an oil phase for later use;

[0045] S3: Mix the peptide solution with a concentration of 10 mg / mL, sodium azide stock solution (0.01 wt%) and Tween 20 (0.5 wt%), and prepare the aqueous phase for use;

[0046] S4: Mix the oil phase and water phase at a ratio of 1:9, and disperse at a high speed of 10000r / min for 3min;

[0047] S5: homogenize 7 times under high pressure of 10000psi. A stable emulsion of β-carotene, an enzymatic hydrolysate of male gonads of Yezo scallop, was obtained.

Embodiment 2

[0049] S1: Use trypsin to enzymolyze the male gonads of the scallop scallop to obtain an enzymatic hydrolysate with a degree of hydrolysis of 1%, which is freeze-dried for later use.

[0050] S2: Heat β-carotene (0.1wt%) at 55°C for 10 minutes, dissolve it in corn oil, and prepare it as an oil phase for later use;

[0051] S3: Mix the peptide solution with a concentration of 20 mg / mL, sodium azide stock solution (0.01 wt%) and Tween 20 (1 wt%), and prepare the aqueous phase for use;

[0052] S4: Mix the oil phase and the water phase at a ratio of 1:9, and disperse at a high speed of 15000r / min for 2min;

[0053] S5: homogenize 6 times under high pressure of 11000psi. A stable emulsion of β-carotene, an enzymatic hydrolysate of male gonads of Yezo scallop, was obtained.

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Abstract

The invention discloses a preparation method of a stable patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion. The preparation method comprises the following steps of (1) performing enzymolysis with trypsin on patinopecten yessoensis male gonads to obtain a zymolyte peptide solution of which the hydrolysis degree is 1%, and performing freeze drying for standby application; (2) heating beta-carotene to enable the beta-carotene to dissolve in corn oil and preparing oil phases for standby application; (3) mixing the peptide solution of certain concentration with a sodium azide stock solution and Tween 20 and preparing aqueous phases for standby application; and (4) mixing the oil phases with the aqueous phases in a certain proportion, and performing high-speed dispersing; and (5) performing high-pressure homogenizing to obtain a stable patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion. The patinopecten yessoensis male gonad zymolyte is used as an emulsifying agent, and the beta-carotene is entrapped, so that the patinopecten yessoensis male gonad zymolyte -beta-carotene emulsion being stable and high in nutrient value can be obtained.

Description

Technical field [0001] The invention relates to a preparation method of an emulsion, and more specifically, to a preparation method of a β-carotene emulsion, an enzymatic hydrolysate of the male gonad of the scallop scallop. Background technique [0002] Fat-soluble nutrients are substances that can be dissolved in fat and then ingested, digested, and absorbed by the body. Common fat-soluble nutrients include fish oil, algae oil, phospholipids, and fat-soluble vitamins. In order to improve the bioavailability of fat-soluble nutrients in the human body, a common method is to embed them in an oil-in-water emulsion system. [0003] When fat-soluble nutrients are delivered as core materials, they can be protected from degradation. Proteins are commonly used as emulsifiers to stabilize the oil-water interface by reducing interfacial tension and increasing steric hindrance; while proteolytic substances have Antioxidant ability can inhibit lipid oxidation and can also stabilize emulsion...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/18A23L29/10
CPCA23V2002/00A23L29/10A23L33/105A23L33/18A23V2200/222A23V2200/30A23V2250/211A23V2250/55A23V2250/18A23V2300/24A23V2300/26
Inventor 唐越姜卉王笑涵吴海涛韩佳润商文慧阎佳楠
Owner DALIAN POLYTECHNIC UNIVERSITY
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