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Tricholoma mongoiicum new strain prairie button mushroom 1 and breeding method thereof

A technology of grassland white mushroom and Mongolian mushroom, applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of non-utilization, extinction of Mongolian mushrooms, pollution, etc., and achieve strong stress resistance. , the expansion of economic growth points, the effect of stable genetic performance

Active Publication Date: 2018-04-27
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to factors such as over-harvesting, desertification and degradation of grass pastures, lack of rain and drought in recent years, Tricholoma mongolica is almost extinct, which has become a great pity on the grasslands today. Around the animal husbandry point, it is not used, resulting in waste of resources and environmental pollution

Method used

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  • Tricholoma mongoiicum new strain prairie button mushroom 1 and breeding method thereof
  • Tricholoma mongoiicum new strain prairie button mushroom 1 and breeding method thereof
  • Tricholoma mongoiicum new strain prairie button mushroom 1 and breeding method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1) production of female species: collect robust wild mushrooms to make female species, cut fresh wild fruiting bodies of mushrooms under aseptic conditions, select the fungal tissue at the junction of caps and stalks, and move into a PDA modified medium. Put it in a constant temperature incubator for cultivation, and cultivate it at a temperature of 20°C to 23°C for 25 to 28 days, until the mycelium is full.

[0050] 2) Propagation: The mother seed was transferred to the PDA modified medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium was covered.

[0051] PDA modified medium is: temperature 121 ℃ ~ 125 ℃ (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) Sterilized for 30 minutes, containing sweet potato 200g / L, sucrose 10g / L, glucose 10g / L, yeast powder 1.6g / L, peptone 1.6g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1g / L, agar 10g / L of medium.

[0052] 3) Original seed production: transplant the obtained multiplication mother seed into a vess...

Embodiment 2

[0057] 1) production of female species: collect robust wild mushrooms to make female species, cut fresh wild fruiting bodies of mushrooms under aseptic conditions, select the fungal tissue at the junction of caps and stalks, and move into a PDA modified medium. (with potato 200g / L, others are the same as in Example 1), put it into a constant temperature incubator for cultivation, and cultivate at a temperature of 20°C to 23°C for 25 to 28 days, until the mycelium is full.

[0058] Propagation: The mother seed is transferred to the improved PDA liquid medium, and cultured at 20°C to 23°C at a speed of 140r / min for 8 to 10 days.

[0059] PDA modified liquid medium is: temperature 121 ℃ ~ 125 ℃ (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) Sterilization for 30 minutes contains 200g / L of potatoes, 10g / L of sucrose, 10g / L of glucose, 1.6g / L of yeast powder, 1.6g / L of peptone, 0.5g / L of magnesium sulfate, and 1g / L of dipotassium hydrogen phosphate.

[0060] 3) Original seed production: trans...

Embodiment 3

[0065] 1) Production of female species: collect robust wild M. mongolica to make mother-type, use iron wire to pass through the fruiting bodies of fresh wild M. mongolica under aseptic conditions, make the gills hang down in the jar to collect basidiospores, pick out the basidiospores. Take a small amount of basidiospores in a test tube, add sterile water to dilute to 100 μL containing 40-60 basidiospores, and obtain a basidiospore suspension. Aspirate the basidiospore suspension and add it dropwise to the modified medium containing PDA (using 100 / L of bran, the others are the same as in Example 1), and evenly spread it, and cultivate it at a temperature of 20°C to 23°C for 25 to 28 days. until the mycelium is overgrown.

[0066] 2) Propagation: The mother seed was transferred to the PDA modified medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium was covered.

[0067] PDA modified medium is: temperature 121 ℃ ~ 125 ℃ (pressure 1.1kg / cm 2 ~1.5kg / cm 2 )...

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Abstract

The invention belongs to the field of edible mushroom new strain domestication methods, and particularly discloses a tricholoma mongoiicum new strain prairie button mushroom 1 and a breeding method thereof. The accession number of the tricholoma mongoiicum new strain prairie button mushroom 1 is CGMCC No. 15085, the tricholoma mongoiicum new strain prairie button mushroom 1 has the characteristicsof high stress resistance, stable heritability, high quality and delicious taste, and a new high-quality edible mushroom is provided for people. By the domestication method, tricholoma mongoiicum which is an endangered rare specy is protected, furthermore, resourceful utilization of husbandry wastes is realized by cultivation of a new strain of a prairie button mushroom 1, sustainable developmentof agricultural economy is promoted, the economic growth point of farmers and herdsmen is expanded, the tricholoma mongoiicum new strain prairie button mushroom 1 is important for development of courtyard economy of a pasturing area and grass and livestock circulation economy, and business starting, employment obtaining and enriching can be realized by cultivation of the prairie button mushroom 1through facilities such as wastes of farming and stockbreeding areas and idle cattle and sheep houses.

Description

technical field [0001] The invention belongs to the field of domestication methods for new strains of edible fungi, in particular to a new strain of Trichoderma mongolica No. Background technique [0002] Mongolian mushroom (Tricholoma mongolicum Imai) belongs to the order Agaricales, Tricholomataceae, Tricholoma or Leucocalcybe, also known as pearl mushroom, commonly known as white mushroom, etc. Mainly distributed in Xilin Gol, Horqin and Hulunbuir grasslands in Inner Mongolia, the white mushrooms in Xilin Gol are of the best quality. Mongolian mushroom is rich in protein, vitamins, potassium, phosphorus, selenium, iron and other minerals and active ingredients such as polysaccharides, polypeptides, and triterpenes. Mongolian mushrooms have tender meat, pure tulip, and excellent taste. They are treasures among bacteria. They also have medical and health care functions, anti-tumor, lowering blood sugar and blood lipids, etc., and are valuable wild edible mushroom resources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A01G18/20A01G18/70C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 郭九峰孙国琴王润元王勇王海燕边淑萍庞杰于传宗李亚娇金焕林
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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