A New Strain of Tricholoma mongolica and Its Breeding Method
A technology of grassland white mushroom and Mongolian mushroom, which is applied to the new strain of Mongolian mushroom No. 1 and its breeding field, can solve the problems of non-utilization, extinction of Mongolian mushroom, pollution, etc., to achieve strong stress resistance and expand economic growth. point, the effect of stable genetic performance
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Embodiment 1
[0049] 1) Production of mother species: collect strong and wild Tricholoma mongolica to make mother species, cut fresh wild Tricholoma mongolica fruiting bodies under aseptic conditions, select the meat tissue at the junction of the cap and stipe, and transfer it into the modified medium containing PDA Put it in a constant temperature incubator and cultivate it in a constant temperature incubator, and cultivate it at a temperature of 20°C to 23°C for 25 to 28 days until the mycelium is overgrown.
[0050] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium is covered.
[0051] The PDA modified medium is: After the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, containing sweet potato 200g / L, sucrose 10g / L, glucose 10g / L, yeast powder 1.6g / L, peptone 1.6g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1g / L, agar 10g / L medium....
Embodiment 2
[0057] 1) Production of mother species: collect strong and wild Tricholoma mongolica to make mother species, cut fresh wild Tricholoma mongolica fruiting bodies under aseptic conditions, select the meat tissue at the junction of the cap and stipe, and transfer it into the modified medium containing PDA (with potato 200g / L, other is with embodiment 1) in the vessel, puts into the constant temperature incubator and cultivates, and cultivates 25~28 days at the temperature of 20 ℃~23 ℃, until mycelium overgrows vessel.
[0058] Propagation: The mother species is transferred to the improved PDA liquid medium, and cultured at 20°C to 23°C at a speed of 140r / min for 8 to 10 days. The cultured balls evenly cover the vessel to complete the production of liquid strains.
[0059] The PDA modified liquid medium is: after the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, containing 200g / L of potatoes, 10g / L of sucrose, 10g / L of glucose, 1.6...
Embodiment 3
[0065] 1) Preparation of mother species: collect strong and wild Tricholoma mongolica to make mother species, pass through fresh wild Tricholoma mongolica fruiting bodies with iron wire under aseptic conditions, make its gills hang downwards in jars to collect basidiospores, pick Take a few basidiospores and put them in a test tube, add sterile water to dilute to 100 μL containing 40-60 basidiospores to obtain basidiospore suspension. Draw the basidiospore suspension and add dropwise to the PDA-improved medium (with bran 100 / L, others are the same as in Example 1), and spread evenly, and cultivate at a temperature of 20°C to 23°C for 25 to 28 days. To mycelia overgrowth.
[0066] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium is covered.
[0067] The PDA modified medium is: After the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, c...
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