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A New Strain of Tricholoma mongolica and Its Breeding Method

A technology of grassland white mushroom and Mongolian mushroom, which is applied to the new strain of Mongolian mushroom No. 1 and its breeding field, can solve the problems of non-utilization, extinction of Mongolian mushroom, pollution, etc., to achieve strong stress resistance and expand economic growth. point, the effect of stable genetic performance

Active Publication Date: 2021-03-23
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to factors such as over-harvesting, desertification and degradation of grass pastures, lack of rain and drought in recent years, Tricholoma mongolica is almost extinct, which has become a great pity on the grasslands today. Around the animal husbandry point, it is not used, resulting in waste of resources and environmental pollution

Method used

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  • A New Strain of Tricholoma mongolica and Its Breeding Method
  • A New Strain of Tricholoma mongolica and Its Breeding Method
  • A New Strain of Tricholoma mongolica and Its Breeding Method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] 1) Production of mother species: collect strong and wild Tricholoma mongolica to make mother species, cut fresh wild Tricholoma mongolica fruiting bodies under aseptic conditions, select the meat tissue at the junction of the cap and stipe, and transfer it into the modified medium containing PDA Put it in a constant temperature incubator and cultivate it in a constant temperature incubator, and cultivate it at a temperature of 20°C to 23°C for 25 to 28 days until the mycelium is overgrown.

[0050] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium is covered.

[0051] The PDA modified medium is: After the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, containing sweet potato 200g / L, sucrose 10g / L, glucose 10g / L, yeast powder 1.6g / L, peptone 1.6g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1g / L, agar 10g / L medium....

Embodiment 2

[0057] 1) Production of mother species: collect strong and wild Tricholoma mongolica to make mother species, cut fresh wild Tricholoma mongolica fruiting bodies under aseptic conditions, select the meat tissue at the junction of the cap and stipe, and transfer it into the modified medium containing PDA (with potato 200g / L, other is with embodiment 1) in the vessel, puts into the constant temperature incubator and cultivates, and cultivates 25~28 days at the temperature of 20 ℃~23 ℃, until mycelium overgrows vessel.

[0058] Propagation: The mother species is transferred to the improved PDA liquid medium, and cultured at 20°C to 23°C at a speed of 140r / min for 8 to 10 days. The cultured balls evenly cover the vessel to complete the production of liquid strains.

[0059] The PDA modified liquid medium is: after the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, containing 200g / L of potatoes, 10g / L of sucrose, 10g / L of glucose, 1.6...

Embodiment 3

[0065] 1) Preparation of mother species: collect strong and wild Tricholoma mongolica to make mother species, pass through fresh wild Tricholoma mongolica fruiting bodies with iron wire under aseptic conditions, make its gills hang downwards in jars to collect basidiospores, pick Take a few basidiospores and put them in a test tube, add sterile water to dilute to 100 μL containing 40-60 basidiospores to obtain basidiospore suspension. Draw the basidiospore suspension and add dropwise to the PDA-improved medium (with bran 100 / L, others are the same as in Example 1), and spread evenly, and cultivate at a temperature of 20°C to 23°C for 25 to 28 days. To mycelia overgrowth.

[0066] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultured at 20°C to 23°C for 25 to 28 days until the mycelium is covered.

[0067] The PDA modified medium is: After the temperature is 121 ° C ~ 125 ° C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) sterilized for 30 minutes, c...

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Abstract

The invention belongs to the field of domestication methods of new strains of edible fungi, and specifically discloses a new strain of Tricholoma mongolicum, Grassland White Mushroom No. 1 and a breeding method thereof. The preservation number of the new strain of Tricholoma mongolica of the present invention is CGMCC No. 1. It has the characteristics of strong stress resistance, stable genetic performance, high quality and delicious taste, and provides people with a new high-quality edible fungus. . The domestication method provided by the present invention not only protects the rare species Tricholoma mongolica which is on the verge of extinction, but also realizes the resource utilization of agricultural and animal husbandry waste through the cultivation of No. The new economic growth point for farmers and herdsmen is an important part of the development of courtyard economy and circular economy of grassland and livestock in pastoral areas. The use of waste from agricultural and pastoral areas, idle cattle and sheep pens and other facilities to cultivate Grassland White Mushroom No. 1 is used to start a business and obtain employment. get rich.

Description

technical field [0001] The invention belongs to the field of domestication methods of new strains of edible fungi, and in particular relates to a new strain of Tricholoma mongolicum, Grassland White Mushroom No. 1 and a breeding method thereof. Background technique [0002] Tricholoma mongolicum Imai belongs to the order Agaricales, the family Tricholomataceae, the genus Tricholoma or the genus Leucocalcybe, also known as pearl mushroom, commonly known as white mushroom and so on. It is mainly distributed on the grasslands of Xilingol, Horqin, and Hulunbeier in Inner Mongolia. The white mushrooms in Xilingol are of the best quality. Tricholoma mongolica is rich in protein, vitamins, potassium, phosphorus, selenium, iron and other minerals, as well as active ingredients such as polysaccharides, polypeptides and triterpenes. Tricholoma mongolica has tender meat, pure tulip, and excellent taste. It is a treasure among bacteria. It also has medical and health care effects, anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01G18/20A01G18/70C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 郭九峰孙国琴王润元王勇王海燕边淑萍庞杰于传宗李亚娇金焕林
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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