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Mass spectrometry method for serum, gold-palladium-based matrix, and preparation method and application of matrix

A mass spectrometry and gold-palladium technology, which is applied in the field of mass spectrometry detection, can solve the problems of difficult detection of small molecules, and achieve the effects of simple pretreatment, simplified detection process, and low matrix cost

Pending Publication Date: 2018-02-02
浙江亿纳谱生命科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned defects of the prior art, the present invention provides a serum mass spectrometry method, a gold-palladium-based matrix and its preparation method and application, so as to solve the problems in the detection of biological samples by the existing matrix-assisted laser desorption ionization mass spectrometry detection method. Small Molecule Detection Difficult Problem

Method used

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  • Mass spectrometry method for serum, gold-palladium-based matrix, and preparation method and application of matrix
  • Mass spectrometry method for serum, gold-palladium-based matrix, and preparation method and application of matrix
  • Mass spectrometry method for serum, gold-palladium-based matrix, and preparation method and application of matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This example proposes a gold-palladium-based matrix containing nanoparticles of a gold-palladium alloy.

[0056] Its production method is as follows.

[0057] Step 1: Using water as a solvent, prepare cetyltrimethylammonium chloride solution, cetyltrimethylammonium bromide solution, trisodium citrate solution, chloroauric acid solution, and chloropalladium acid solution;

[0058] Step 2: Mix the above solutions and stir at room temperature until the solutions are evenly dispersed to obtain a mixed solution;

[0059] Step 3: move the mixed solution obtained in step 2 to a polytetrafluoroethylene-lined stainless steel hydrothermal reactor, and keep it at 90°C for 16 hours to form gold-palladium alloy nanoparticles;

[0060] Step 4: repeatedly washing the gold-palladium alloy nanoparticles obtained in step 3 with deionized water, and drying them into powder at 60° C.;

[0061] Step 5: resuspend (resuspend) the dried powdered gold-palladium alloy nanoparticles in deionize...

Embodiment 2

[0068] This implementation is a typical application example of using a matrix to perform matrix-assisted laser desorption ionization mass spectrometry detection on biological samples.

[0069] A gold-palladium-based matrix is ​​used to detect various small molecule standards, wherein the small molecule standards include: glucose standards, mannitol standards, lysine standards, and tryptophan standards.

[0070] The detection method is as follows:

[0071] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. Data Explorer was used to observe, process, and analyze data, and only mass spectrometer signals with a sign...

Embodiment 3

[0080] For the detection of serum samples, the detection method process can be found in image 3 .

[0081] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis.

[0082] (2) Dilute the serum in proportion.

[0083] (3) Take 0.5 μL of serum solutions with different dilution ratios, and spot them on the target plate for detection.

[0084] (4) Point a matrix containing gold-palladium alloy nanoparticles on the sample, and dry at room temperature.

[0085] (5) Analyze the detection results of mass spectrometry and draw conclusions.

[0086] Mass spectrometry results such as Figure 4 shown.

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Abstract

The invention discloses a mass spectrometry method for serum, a gold-palladium-based matrix, and a preparation method and an application of the matrix, and belongs to the field of mass spectrometry detection. The gold-palladium-based matrix comprises a nano-particle aggregation, above nano-particles contain the gold element and the palladium element, the size of the nano-particles is 100 nm or below, and the granularity of the nano-particles in the aggregation has monodispersion. The matrix can effectively remove background noise interference of traditional organic matrixes and greatly improvethe desorption and ionization effects of micro-molecular substances. The new method for analyzing micro-molecular metabolites in a serum system based on the matrix avoids sample pretreatment in the process, and reduces the serum sample to the level of dozens of microliter in order to realize the rapid and highly-sensitive detection of the micro-molecular metabolites.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to a serum mass spectrometry analysis method, a gold-palladium-based substrate and a preparation method and application thereof. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry is a new type of soft ionization biological sample analysis method, which can analyze substances of different molecular weight levels. It represents a simple, rapid, and precise way to simultaneously detect multiple biomolecules. The effectiveness of this method has been confirmed not only in theory but also in practical application. The core part of the instrument mainly includes a matrix-assisted laser desorption ionization source and a mass analyzer. In the matrix-assisted laser desorption ionization source, when the laser is irradiated on the co-crystal formed by the matrix and the analyte, the matrix will absorb most of the laser energy, and then tr...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/44G01N1/40
CPCG01N1/4055G01N1/44G01N27/628
Inventor 万馨泽刘彬
Owner 浙江亿纳谱生命科技有限公司
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