Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Oat bran active polysaccharide extraction and purification method

A technology for oat bran and active polysaccharide, which is applied in the field of extraction and purification of active polysaccharide from oat bran, can solve the problems of complex process, high operation cost, long extraction time and the like, and achieves simple extraction method, low operation cost and short extraction time. Effect

Inactive Publication Date: 2018-01-05
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hot water extraction method has disadvantages such as long extraction time, low yield, and serious waste of resources; alkaline extraction has a large amount of salt and other impurities in the process of adjusting the pH, which is not conducive to purification and strong alkaline components may affect the functional activity of polysaccharides. ;Although the extraction rate of the acid extraction method is high, it will significantly reduce the viscosity of β-glucan and generate a large amount of heat. If it is used in production, the heat dissipation problem must be considered, and it is difficult in practical application; the enzyme extraction method can effectively improve the polysaccharide. yield, and the conditions are mild, so that the biological activity of the polysaccharide is not destroyed, but the disadvantage is that the cost of the enzyme preparation is high, and it is not easy to realize industrial production
Therefore, it is of great significance to research and invent a new and effective extraction process to prepare oat bran polysaccharides to overcome the shortcomings of the original technology, such as long extraction time, high operating cost, complicated process, and low activity of extracting oat bran polysaccharides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oat bran active polysaccharide extraction and purification method
  • Oat bran active polysaccharide extraction and purification method
  • Oat bran active polysaccharide extraction and purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The extraction and purification method of oat bran active polysaccharide, the specific implementation steps are as follows:

[0055] a. Pretreatment: remove impurities from oat bran, dry at 40°C, and grind to 30 mesh to obtain pretreated oat bran;

[0056] b. Degreasing:

[0057] (1) Ethanol degreasing:

[0058] Add 85% 350 mL of ethanol to 70 g of oat bran pretreated in step a, heat and reflux at 60 °C and stir for 1.5 h, cool to room temperature and continue stirring for 10 h, remove the liquid, add 85 % 350 mL of ethanol, and stir at room temperature 3 h, remove the liquid, and make the first defatted oat bran;

[0059] (2) Acetone degreasing:

[0060] Add 99 % 150 mL of acetone solution to the primary defatted oat bran prepared in step (1), stir for 30 minutes, then take the precipitate and dry it at room temperature for 12 hours to obtain defatted oat bran;

[0061] c. Polysaccharide extraction:

[0062] Add 50 g of defatted oat bran prepared in step b to 1000...

Embodiment 2

[0076] The extraction and purification method of oat bran active polysaccharide, the specific implementation steps are as follows:

[0077] a. Pretreatment: remove impurities from oat bran, dry at 60°C, and grind to 80 mesh to obtain pretreated oat bran;

[0078] b. Degreasing:

[0079] (1) Ethanol degreasing:

[0080] Add 85% 1050 mL of ethanol to 70 g of oat bran pretreated in step a, heat and reflux at 80 °C for 3 h, cool to room temperature and continue stirring for 18 h, remove the liquid, add 85 % 1050 mL of ethanol, and stir at room temperature for 7 h h, removing the liquid to obtain the first defatted oat bran;

[0081] (2) Acetone degreasing:

[0082] Add 99 % 560 mL of acetone solution to the primary defatted oat bran prepared in step (1), stir for 30 minutes, then take the precipitate and dry it at room temperature for 12 hours to obtain defatted oat bran;

[0083] c. Polysaccharide extraction:

[0084] Add 50 g of defatted oat bran prepared in step b to 2800 ...

Embodiment 3

[0098] The extraction and purification method of oat bran active polysaccharide, the specific implementation steps are as follows:

[0099] a. Pretreatment: remove impurities from oat bran, dry at 45°C, and grind to 40 mesh to obtain pretreated oat bran;

[0100] b. Degreasing:

[0101] (1) Ethanol degreasing:

[0102] Add 70 g of oat bran pretreated in step a, add 85 % 700 mL of ethanol, heat and reflux at 60 °C and stir for 1.5 h, cool to room temperature and continue stirring for 10 h, remove the liquid, add 85 % 350 mL of ethanol, and stir at room temperature 3 h, remove the liquid, and make the first defatted oat bran;

[0103] (2) Acetone degreasing:

[0104] Add 99 % 280 mL of acetone solution to the primary defatted oat bran prepared in step (1), stir for 30 minutes, then take the precipitate and dry it at room temperature for 12 hours to obtain defatted oat bran;

[0105] c. Polysaccharide extraction:

[0106] Add 50 g of defatted oat bran prepared in step b to 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of oat bran deep-processing, and particularly relates to an oat bran active polysaccharide extraction and purification method. The method includes the steps that polysaccharide is extracted through preprocessing, degreasing and ultrasound-microwave synergistic extraction, and finally the yield, the purity, monosaccharide composition, the antioxidant activity andthe antilipidemia activity of the extracted oat bran active polysaccharide are analyzed. According to the oat bran active polysaccharide extraction and purification method, an oat bran active polysaccharide mixture is extracted by means of an ultrasound-microwave synergistic method, the polysaccharide with the antioxidant and antilipidemia regulating activity is obtained through dialysis, vacuum concentration and freeze-drying, the extraction method is simple, the operation cost is low, extraction duration is short, the yield and the purity of the obtained oat bran active polysaccharide are 8.45% and 93.2% respectively, and the oat bran active polysaccharide has good antioxidant and antilipidemia regulating activity.

Description

technical field [0001] The invention belongs to the technical field of oat bran deep processing, and in particular relates to a method for extracting and purifying active polysaccharides from oat bran. Background technique [0002] Oat bran is the outer tissue of oats obtained after cleaning oats or oatmeal after grinding and sieving. However, the structure of oats is soft, and the bran and endosperm cannot be completely separated, so oat bran contains more endosperm powder. Compared with other grain bran, oat bran is rich in water-soluble dietary fiber. Fiber is an important functional polysaccharide. But at present, oat bran has not been deeply developed for its health function except for a little use in bread, granules, etc., and its economic benefits are low. Therefore, extracting biologically active substances from oat bran is helpful for increasing the economic value of oat bran It is particularly important to improve the social benefits of agricultural and sideline ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张丽萍李朝阳赵慧霞郭增旺
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com