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Soft-lithospermum total phenolic acid and use thereof in pharmacy

A technology of soft comfrey and total phenolic acids, which is applied in the direction of pharmaceutical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of complex extraction and purification methods of caffeic acid tetramers, which are not suitable for industrialization Production, high cost and other issues, to achieve the effect of increasing drug stability, quality control, and avoiding waste of resources

Inactive Publication Date: 2011-07-20
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, the methods for extracting and purifying caffeic acid tetramer in relevant literatures in the prior art are complex, expensive and have extremely low yields, which are not suitable for the needs of large-scale industrial production.

Method used

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  • Soft-lithospermum total phenolic acid and use thereof in pharmacy
  • Soft-lithospermum total phenolic acid and use thereof in pharmacy
  • Soft-lithospermum total phenolic acid and use thereof in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take the soft comfrey root dried at 60-70°C, crush it at 30-80 mesh, add 10 times the volume of water (HCl to adjust pH3.0), and extract at 50°C for 3 hours each time, and extract 3 times in total. Filter twice with qualitative filter paper and 0.45 μm microporous membrane, and further purify with macroporous adsorption resin. Using LK001 resin, dynamic adsorption method on the column, the flow rate is 10BV / h. After absorbing near saturation, wash the column quickly with deionized water and 20% ethanol until it is colorless, and elute with 45% ethanol to light yellow. have to. Among them, the content of effective parts of total phenolic acid of soft comfrey is 80.5%, and the content of index components of caffeic acid tetramer is 21%.

Embodiment 2

[0037]Take the soft comfrey roots dried at 60-70°C, crush them at 30-80 meshes, add 10 times the volume of water (dilute HCl to adjust the pH to 3.0), extract at 50°C for 3 hours each time, and extract 3 times in total, and combine the extracts, Filter twice with qualitative filter paper and 0.45 μm microporous membrane, and further purify with macroporous adsorption resin. AB8 type resin is used, and the dynamic adsorption method is applied to the column, and the flow rate is 10BV / h. After absorbing near saturation, wash the column quickly with deionized water and 20% ethanol respectively, and elute with 45% ethanol to light yellow. Total phenolic acids of shikon. Among them, the content of effective fractions of total phenolic acids of soft comfrey is 80.9%, and the content of caffeic acid tetramer index components is 21.2%.

Embodiment 3

[0039] Take soft comfrey roots dried at 60-70°C, crush them at 30-80 meshes, add 10 times the volume of 10% ethanol (dilute HCl to adjust the pH to 3.0), and extract at 50°C for 3 hours each time, a total of 3 extractions, combined extraction The solution was filtered twice with qualitative filter paper and 0.45 μm microporous membrane, and further purified with macroporous adsorption resin. LK001 type resin is used, and the dynamic adsorption method is applied to the column, and the flow rate is 10BV / h. After absorbing near saturation, wash the column quickly with deionized water and 20% ethanol respectively, and elute with 50% ethanol until light yellow. Total phenolic acids of shikon. Among them, the content of effective fractions of total phenolic acids of soft comfrey is 81%, and the content of caffeic acid tetramer index components is 21.3%.

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Abstract

The invention belongs to the field of Chinese medicaments, and in particular relates to a soft-lithospermum total phenolic acid which is extracted from soft-lithospermum and contains effective fractions of the soft-lithospermum total phenolic acid (taking caffeic acid tetramers as index components), as well as use thereof in pharmacy. The soft-lithospermum total phenolic acid is taken as a medicinal active component and is prepared into anti-HIV injection medicaments, including small-volume soft-lithospermum total phenolic acid injection agents and soft-lithospermum total phenolic acid freeze-dried powder injection agents, wherein the content of the soft-lithospermum total phenolic acid is up to 80 percent, and the caffeic acid tetramers are taken as the index components and have the content not less than 20 percent. Experiments prove that the injection agents have the characteristic of the high blood concentration of the caffeic acid tetramers serving as active components, are controllable in quality, and meet the modernization requirements of Chinese medicaments. The invention also provides a method for preparing the small-volume soft-lithospermum total phenolic acid injection agents and the soft-lithospermum total phenolic acid freeze-dried powder injection agents.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to extracting the total phenolic acid of the soft comfrey containing the effective part of the total phenolic acid of the soft comfrey (caffeic acid tetramer is an index component) and its application in pharmacy. Background technique [0002] AIDS is a chronic fatal infectious disease caused by human immunodeficiency virus (HIV) infection. Studies have shown that the main target cell of HIV is CD4 cell. After HIV ribonucleic acid (RNA) enters the target cell, it first needs to be converted into deoxyribonucleic acid (DNA) under the action of reverse transcriptase, then translate, manufacture, assemble the viral protein, and finally complete its replication process. As the disease progresses, CD4 cells decline progressively, leading to a decline in immune function and even a breakdown of the immune system. The goals of modern treatment mainly include virolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K47/10A61K9/19A61P31/18A61K36/30
Inventor 沙先谊李雅娟方晓玲
Owner FUDAN UNIV
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