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A method for extracting and purifying residual estrogen in animal samples

An animal and estrogen technology, applied in the field of detection of residual estrogen in animal samples, extraction and purification of residual estrogen in animal samples, can solve the problems of high cost and need of SPE column, and achieve low cost and purification effect Good, fast detection effect

Active Publication Date: 2020-11-03
CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The SPE method is widely used in food analysis based on its high enrichment capacity and good purification effect, but the cost of the SPE column is high, and a solid phase extraction device is required

Method used

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  • A method for extracting and purifying residual estrogen in animal samples
  • A method for extracting and purifying residual estrogen in animal samples
  • A method for extracting and purifying residual estrogen in animal samples

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Instrument

[0040] Ultra-high performance liquid chromatography LC-30A and triple quadrupole mass spectrometer TQ 8050 (manufactured by Shimadzu Corporation, Japan).

[0041] 2. Instrument Analysis Conditions

[0042] Chromatographic conditions: mobile phase A is 0.1v / v% ammonia solution, and B is methanol solution. Gradient elution: 0~3min, 60v / v%B; 3~10min, 60v / v%~100v / v%B; 10~12min, 100v / v%B; 12~15min, 100v / v%~60v / v %B. Injection volume: 10μL; flow rate 300μL / min; column temperature: 40℃; Waters ACQUITY UPLC BEH C 18 Chromatography column (1.7μm, 2.1×100mm).

[0043] Mass spectrometry conditions: electrospray ionization, negative ion mode, atomization gas flow rate of 3 L / min; heating gas flow rate of 10 L / min; interface temperature of 300 °C; solvent removal tube temperature of 250 °C; heating block temperature of 400 °C °C; the drying gas flow rate is 10 L / min; the scanning mode is dynamic multiple reaction monitoring (MRM).

[0044] 3. Estrogen standard...

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Abstract

The invention relates to a method for extracting and purifying residual female hormones in an animal sample. The method comprises the following steps: (1) extracting residual female hormones in an animal sample by a solvent (A) to obtain an extract; and (2) adopting a strong anion exchange filling material (PAX) as the solid adsorbent, and enriching and purifying the residual female hormones in the extract by adopting a dispersant solid phase micro extraction method. The provided extraction and purification method has the advantages of simpleness, rapidness, convenience, and low cost.

Description

technical field [0001] The invention belongs to the field of food safety, in particular to a method for extracting and purifying residual estrogen in animal samples, and also relates to a method for detecting residual estrogen in animal samples. Background technique [0002] Hormone drugs include estrogen, progesterone, androgen, and environmental hormones. Because estrogen has the effect of affecting animal sex differentiation and shortening the growth cycle of animals, it is often added to feed by unscrupulous farmers to improve feeding efficiency. Residual estrogens, such as estradiol, estriol, estrone, ethinyl estradiol, diethylstilbestrol, hexestrol, and diethylstilbestrol, are often detected in food of animal origin. This type of estrogen is generally stable and difficult to be degraded. After entering the human body through the food chain, it still has strong biological activity and potential carcinogenicity. [0003] [0004] When detecting residual estrogen sam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/08
CPCG01N30/08G01N2030/027G01N2030/062
Inventor 陈达炜王竹天赵云峰
Owner CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT
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